Disease relevance of DUGVsSgp1

• This can encode a 483 amino acid polypeptide, M(r) 53.9 kDa, corresponding to the viral nucleocapsid protein N. The DUG N protein is thus similar in length to the N proteins of Hazara (HAZ) and Crimean-Congo haemorrhagic fever (CCHF) nairoviruses, which are 485 and 482 amino acids in length, respectively [1].
• N protein trimers from the Andes, Puumala, Prospect Hill, Seoul, and Sin Nombre viruses recognize their individual homologous panhandles as well as other hantavirus panhandles with high affinity [2].
• The 49.4-kDa product was expressed as a fusion protein with beta-galactosidase in Escherichia coli cells and confirmed as DUG N protein by Western blotting with DUG N-specific monoclonal antibody [3].
• The nucleocapsid protein N accumulated in scattered foci throughout the cytoplasm, and this appears to be related to the limited maturation of DUG virus particles that occurred [4].
• Monoclonal and polyclonal antibodies to the N protein of Crimean-Congo haemorrhagic fever (CCHF) virus were found to cross-react weakly with the baculovirus expressed DUG N protein by Western blotting [5].

High impact information on DUGVsSgp1

• Trimeric hantavirus N protein specifically recognizes the panhandle structure formed by complementary base sequence of 5' and 3' ends of viral genomic RNA [2].
• Their S RNA genome segments and encoded nucleocapsid proteins were found to be 1590 nucleotides and 482 amino acids in length and differed by only 10 and 3% at nucleotide and amino acid levels, respectively [6].
• The persistent virus from one cell line, C6/36-PI LO, had a slower migrating nucleocapsid protein on polyacrylamide gels [7].
• Expression of the nucleocapsid protein of Dugbe virus and antigenic cross-reactions with other nairoviruses [5].
• When used in an enzyme linked immunoassay (ELISA), the DUG N protein reacted with polyclonal mouse immune ascitic fluids raised against either CCHF or Hazara viruses (both members of the CCHF serogroup of nairoviruses) [5].

Analytical, diagnostic and therapeutic context of DUGVsSgp1

• Western and Northern blot analyses showed significantly lower DUGV nucleocapsid (N) protein expression and DUGV genomic RNA, respectively, in the presence of MxA [8].

References