Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

SPCS2 - signal peptidase complex subunit 2 homolog...

Homo sapiens

Synonyms: KIAA0102, MGC117366, Microsomal signal peptidase 25 kDa subunit, Signal peptidase complex subunit 2, SPase 25 kDa subunit, ...

Greenburg, G. et al., Bharadwaj, R. et al., Kalies, K.U. et al., Llorent-Martínez, E.J. et al., DeLuca, J.G. et al.

DeLuca, Dong, Hergert, Strauss, Hickey, Salmon, McEwen,

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High impact information on SPCS2

The Ndc80 complex, comprised of Ndc80 (Hec1), Nuf2, Spc24, and Spc25, is essential for metaphase chromosome alignment and anaphase chromosome segregation [1].
RNA interference-mediated depletion of hSPC25 in HeLa cells caused aberrant mitosis, followed by cell death, a phenotype similar to that of cells depleted of HEC1 [2].
Thus, hSPC25 is an essential kinetochore component that plays a significant role in proper execution of mitotic events [2].
We have determined the cDNA sequence of the remaining 12-kDa subunit (SPC12) as well as the membrane topologies of SPC12 and SPC25 in rough microsomes [3].
Consistent with being the only SPC subunit with at least one intrachain disulfide bond, SPC 25 contains 4 Cys residues [4].

Biological context of SPCS2

We purified the 25-kDa subunit of the pentameric signal peptidase complex (SPC 25) from canine pancreas microsomes and utilized amino acid sequence data from tryptic fragments for its molecular cloning and sequencing [4].

Other interactions of SPCS2

Therefore, SPC12 and SPC25 are likely to be involved in processes other than the enzymatic cleavage of the signal sequence [3].

Analytical, diagnostic and therapeutic context of SPCS2

A minicolumn filled with Sephadex SPC-25 microbeads was used to achieve separation of both analytes before detection in a flow-through cell filled with the same resin [5].

References

Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. DeLuca, J.G., Dong, Y., Hergert, P., Strauss, J., Hickey, J.M., Salmon, E.D., McEwen, B.F. Mol. Biol. Cell (2005)
Identification of two novel components of the human NDC80 kinetochore complex. Bharadwaj, R., Qi, W., Yu, H. J. Biol. Chem. (2004)
Membrane topology of the 12- and the 25-kDa subunits of the mammalian signal peptidase complex. Kalies, K.U., Hartmann, E. J. Biol. Chem. (1996)
cDNA-derived primary structure of the 25-kDa subunit of canine microsomal signal peptidase complex. Greenburg, G., Blobel, G. J. Biol. Chem. (1994)
Multicommuted flow-through fluorescence optosensor for determination of furosemide and triamterene. Llorent-Martínez, E.J., Ortega-Barrales, P., Molina-Díaz, A. Analytical and bioanalytical chemistry. (2005)

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Y37D8A.10	Caenorhabditis elegans
AgaP_AGAP011842	Anopheles gambiae str. PEST
Spase25	Drosophila melanogaster
spcs2	Danio rerio
SPCS2	Gallus gallus
Spcs2	Rattus norvegicus
Spcs2	Mus musculus
SPCS2	Bos taurus
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SPCS2	Pan troglodytes