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MeSH Review

Two-Hybrid System Techniques

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Disease relevance of Two-Hybrid System Techniques


High impact information on Two-Hybrid System Techniques


Chemical compound and disease context of Two-Hybrid System Techniques


Biological context of Two-Hybrid System Techniques


Anatomical context of Two-Hybrid System Techniques


Associations of Two-Hybrid System Techniques with chemical compounds


Gene context of Two-Hybrid System Techniques

  • Each protein contains a CRIB (Cdc42/Rac-interactive binding) motif and each interacts in the two-hybrid assay with the GTP-bound form of the Rho-type Cdc42 GTPase, a key regulator of polarized growth in yeast [26].
  • Using the two-hybrid method, both Dig1 and Dig2 also interact with the other MAP kinase of the pheromone response pathway, Fus3 [27].
  • To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait [28].
  • We have explored interactions between these gene products at the molecular level and find that Oskar interacts directly with Vasa and Staufen, in a yeast two-hybrid assay [29].
  • GAG3 encodes a novel WD40-repeat protein previously found to interact with Dnm1p in a two-hybrid assay [30].

Analytical, diagnostic and therapeutic context of Two-Hybrid System Techniques


  1. The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Stutz, F., Kantor, J., Zhang, D., McCarthy, T., Neville, M., Rosbash, M. Genes Dev. (1997) [Pubmed]
  2. Disruption of PML subnuclear domains by the acidic IE1 protein of human cytomegalovirus is mediated through interaction with PML and may modulate a RING finger-dependent cryptic transactivator function of PML. Ahn, J.H., Brignole, E.J., Hayward, G.S. Mol. Cell. Biol. (1998) [Pubmed]
  3. A yeast two-hybrid assay provides a simple way to evaluate the vast majority of hMLH1 germ-line mutations. Kondo, E., Suzuki, H., Horii, A., Fukushige, S. Cancer Res. (2003) [Pubmed]
  4. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. Taniguchi, N., Taniura, H., Niinobe, M., Takayama, C., Tominaga-Yoshino, K., Ogura, A., Yoshikawa, K. J. Biol. Chem. (2000) [Pubmed]
  5. The human papillomavirus E7 oncoprotein functionally interacts with the S4 subunit of the 26 S proteasome. Berezutskaya, E., Bagchi, S. J. Biol. Chem. (1997) [Pubmed]
  6. The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. Malone, C.J., Misner, L., Le Bot, N., Tsai, M.C., Campbell, J.M., Ahringer, J., White, J.G. Cell (2003) [Pubmed]
  7. CDKN2A mutations in multiple primary melanomas. Monzon, J., Liu, L., Brill, H., Goldstein, A.M., Tucker, M.A., From, L., McLaughlin, J., Hogg, D., Lassam, N.J. N. Engl. J. Med. (1998) [Pubmed]
  8. The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase. Preker, P.J., Lingner, J., Minvielle-Sebastia, L., Keller, W. Cell (1995) [Pubmed]
  9. Smad4 and FAST-1 in the assembly of activin-responsive factor. Chen, X., Weisberg, E., Fridmacher, V., Watanabe, M., Naco, G., Whitman, M. Nature (1997) [Pubmed]
  10. Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p. Whiteway, M.S., Wu, C., Leeuw, T., Clark, K., Fourest-Lieuvin, A., Thomas, D.Y., Leberer, E. Science (1995) [Pubmed]
  11. Physical interaction between hepatitis C virus NS4B protein and CREB-RP/ATF6beta. Tong, W.Y., Nagano-Fujii, M., Hidajat, R., Deng, L., Takigawa, Y., Hotta, H. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  12. Presenilin 1 suppresses the function of c-Jun homodimers via interaction with QM/Jif-1. Imafuku, I., Masaki, T., Waragai, M., Takeuchi, S., Kawabata, M., Hirai, S., Ohno, S., Nee, L.E., Lippa, C.F., Kanazawa, I., Imagawa, M., Okazawa, H. J. Cell Biol. (1999) [Pubmed]
  13. Complex formation by the Drosophila MSL proteins: role of the MSL2 RING finger in protein complex assembly. Copps, K., Richman, R., Lyman, L.M., Chang, K.A., Rampersad-Ammons, J., Kuroda, M.I. EMBO J. (1998) [Pubmed]
  14. Functional characterization of the KNOLLE-interacting t-SNARE AtSNAP33 and its role in plant cytokinesis. Heese, M., Gansel, X., Sticher, L., Wick, P., Grebe, M., Granier, F., Jurgens, G. J. Cell Biol. (2001) [Pubmed]
  15. Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae. Ghislain, M., Dohmen, R.J., Levy, F., Varshavsky, A. EMBO J. (1996) [Pubmed]
  16. PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway. Quattrocchio, F., Verweij, W., Kroon, A., Spelt, C., Mol, J., Koes, R. Plant Cell (2006) [Pubmed]
  17. RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells. Chang, B.Y., Conroy, K.B., Machleder, E.M., Cartwright, C.A. Mol. Cell. Biol. (1998) [Pubmed]
  18. Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein. Izawa, I., Nishizawa, M., Ohtakara, K., Ohtsuka, K., Inada, H., Inagaki, M. J. Biol. Chem. (2000) [Pubmed]
  19. RanBPM, a nuclear protein that interacts with and regulates transcriptional activity of androgen receptor and glucocorticoid receptor. Rao, M.A., Cheng, H., Quayle, A.N., Nishitani, H., Nelson, C.C., Rennie, P.S. J. Biol. Chem. (2002) [Pubmed]
  20. Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptors. Wang, Q., Blackford, J.A., Song, L.N., Huang, Y., Cho, S., Simons, S.S. Mol. Endocrinol. (2004) [Pubmed]
  21. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C zeta-interacting protein. Kuroda, S., Nakagawa, N., Tokunaga, C., Tatematsu, K., Tanizawa, K. J. Cell Biol. (1999) [Pubmed]
  22. Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification. Kurtzman, A.L., Schechter, N. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  23. A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Huang, J., Schreiber, S.L. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  24. Promotion of agonist activity of antiandrogens by the androgen receptor coactivator, ARA70, in human prostate cancer DU145 cells. Miyamoto, H., Yeh, S., Wilding, G., Chang, C. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  25. Association of the Arabidopsis CTR1 Raf-like kinase with the ETR1 and ERS ethylene receptors. Clark, K.L., Larsen, P.B., Wang, X., Chang, C. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  26. The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Chen, G.C., Kim, Y.J., Chan, C.S. Genes Dev. (1997) [Pubmed]
  27. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Cook, J.G., Bardwell, L., Kron, S.J., Thorner, J. Genes Dev. (1996) [Pubmed]
  28. A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing. Dominski, Z., Erkmann, J.A., Yang, X., Sànchez, R., Marzluff, W.F. Genes Dev. (2002) [Pubmed]
  29. Oskar protein interaction with Vasa represents an essential step in polar granule assembly. Breitwieser, W., Markussen, F.H., Horstmann, H., Ephrussi, A. Genes Dev. (1996) [Pubmed]
  30. Gag3p, an outer membrane protein required for fission of mitochondrial tubules. Fekkes, P., Shepard, K.A., Yaffe, M.P. J. Cell Biol. (2000) [Pubmed]
  31. N-terminal domain-mediated homodimerization is required for photoreceptor activity of Arabidopsis CRYPTOCHROME 1. Sang, Y., Li, Q.H., Rubio, V., Zhang, Y.C., Mao, J., Deng, X.W., Yang, H.Q. Plant Cell (2005) [Pubmed]
  32. ERK MAP kinase links cytokine signals to activation of latent HIV-1 infection by stimulating a cooperative interaction of AP-1 and NF-kappaB. Yang, X., Chen, Y., Gabuzda, D. J. Biol. Chem. (1999) [Pubmed]
  33. Interaction of WW domains with hematopoietic transcription factor p45/NF-E2 and RNA polymerase II. Gavva, N.R., Gavva, R., Ermekova, K., Sudol, M., Shen, C.J. J. Biol. Chem. (1997) [Pubmed]
  34. Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via yeast two-hybrid method. Zhang, H.T., Yan, Z.Q., Hu, X.B., Yang, S.L., Gong, Y. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (2003) [Pubmed]
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