The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Cell Culture Techniques

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of Cell Culture Techniques

 

Psychiatry related information on Cell Culture Techniques

 

High impact information on Cell Culture Techniques

  • In rheumatoid joint cell cultures that spontaneously produce IL-1, TNF alpha was the major dominant regulator of IL-1 [11].
  • Often, in cells grown in cell culture, drug resistance correlates with the presence of one or more of the so-called P-glycoproteins or multidrug resistance proteins, products of the mdr family of genes [12].
  • Using a mammalian cell culture system, we demonstrate that recycling endosomes are essential for Delta activity [13].
  • Furthermore, loss of dynein function resulted in premature aggregate formation by mutant huntingtin and increased levels of the autophagosome marker LC3-II in both cell culture and mouse models, compatible with impaired autophagosome-lysosome fusion [14].
  • Recent work using three-dimensional cell culture models underscores the importance of the interactions between epithelial cells-both normal and malignant-and the extracellular matrix that surrounds them in informing life and death decisions [15].
 

Chemical compound and disease context of Cell Culture Techniques

 

Biological context of Cell Culture Techniques

  • In many human cancers and transformed cell lines, Stat3 is persistently activated, and in cell culture, active Stat3 is either required for transformation, enhances transformation, or blocks apoptosis [20].
  • Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell cultures [21].
  • MEIS1 is able to rescue hth mutant phenotypes and can induce the cytoplasmic-to-nuclear translocation of EXD in cell culture and Drosophila embryos [22].
  • Telomere shortening in human and mouse tissues and primary cell cultures may be due to the absence of telomerase activity [23].
  • As demonstrated here, two polyadenylated jockey transcripts detected at different stages of Drosophila ontogenesis and in cell cultures have the same length as genomic copies of jockey and correspond to the strand containing ORFs. alpha-amanitin experiments indicate that jockey is transcribed by RNA polymerase II [24].
 

Anatomical context of Cell Culture Techniques

 

Associations of Cell Culture Techniques with chemical compounds

 

Gene context of Cell Culture Techniques

  • Recombinant TGF-beta1 stimulated Abeta clearance in microglial cell cultures [35].
  • Here we show that tPA upregulates MMP-9 in cell culture and in vivo [36].
  • We demonstrate this specific cleavage in cell culture, in neonatal mouse skin and with recombinant Dsg1, and conclude that Dsg1 is the specific receptor for exfoliative toxin A cleavage [37].
  • Here we have established cell cultures from the brain of an individual with VWM carrying mutations in subunit 5 of eIF2B (encoded by EIF2B5) [38].
  • RANTES and other chemokines that interact with CCR5 and block infection of peripheral blood mononuclear cell cultures inhibit infection of primary macrophages inefficiently at best [39].
 

Analytical, diagnostic and therapeutic context of Cell Culture Techniques

  • I report here that in the dispersed cell culture of chicken pineal gland, N-acetyltransferase activity exhibits a circadian rhythm and responds to environmental lighting in the same manner as in the organ culture [40].
  • These secreted vesicles, named exosomes, were isolated from the cell culture media by differential centrifugation followed by flotation on sucrose density gradients [41].
  • Messenger RNA from adult rat anterior pituitary fragments and cell cultures was reverse transcribed and subjected to PCR amplification using primers specific to the rat CRH gene [42].
  • COX-2 expression and activity were determined on pooled cell cultures by reverse transcription-polymerase chain reaction and prostaglandin E(2) (PGE(2)) enzyme immunoassay, respectively [43].
  • Twenty-five volunteers were randomly assigned to subcutaneous injections of the recombinant (three groups [A, B, and C] received two doses each of 10(3.5), 10(4.5), and 10(5.5) tissue-culture infectious doses50, respectively) or of human diploid cell culture vaccine (HDC; 6.52 international potency units per dose) [44].

References

  1. Characterization of a cell culture model for the study of adenosine deaminase- and purine nucleoside phosphorylase-deficient immunologic disease. Ullman, B., Cohen, A., Martin, D.W. Cell (1976) [Pubmed]
  2. Abelson leukemia virus induces lymphoid and erythroid colonies in infected fetal cell cultures. Waneck, G.L., Rosenberg, N. Cell (1981) [Pubmed]
  3. A potent peptidomimetic inhibitor of HSV ribonucleotide reductase with antiviral activity in vivo. Liuzzi, M., Déziel, R., Moss, N., Beaulieu, P., Bonneau, A.M., Bousquet, C., Chafouleas, J.G., Garneau, M., Jaramillo, J., Krogsrud, R.L. Nature (1994) [Pubmed]
  4. Gonadotroph cell adenomas of the pituitary. Snyder, P.J. Endocr. Rev. (1985) [Pubmed]
  5. Forskolin and phorbol esters reduce the same potassium conductance of mouse neurons in culture. Grega, D.S., Werz, M.A., Macdonald, R.L. Science (1987) [Pubmed]
  6. Vitamin E and Alzheimer disease: the basis for additional clinical trials. Grundman, M. Am. J. Clin. Nutr. (2000) [Pubmed]
  7. gp120 of HIV-1 induces apoptosis in rat cortical cell cultures: prevention by memantine. Müller, W.E., Schröder, H.C., Ushijima, H., Dapper, J., Bormann, J. Eur. J. Pharmacol. (1992) [Pubmed]
  8. Huntington's disease: the neuroexcitotoxin aspartate is increased in platelets and decreased in plasma. Reilmann, R., Rolf, L.H., Lange, H.W. J. Neurol. Sci. (1994) [Pubmed]
  9. Effects of cardioactive drugs on the beating activity of myocardial cell cultures isolated from offspring of trained and untrained pregnant rats. Butler, A.W., Farrar, R.P., Acosta, D. In vitro. (1984) [Pubmed]
  10. Gene expression patterns vary in clonal cell cultures from Rett syndrome females with eight different MECP2 mutations. Traynor, J., Agarwal, P., Lazzeroni, L., Francke, U. BMC Med. Genet. (2002) [Pubmed]
  11. Role of cytokines in rheumatoid arthritis. Feldmann, M., Brennan, F.M., Maini, R.N. Annu. Rev. Immunol. (1996) [Pubmed]
  12. Kinetics of the multidrug transporter (P-glycoprotein) and its reversal. Stein, W.D. Physiol. Rev. (1997) [Pubmed]
  13. Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Emery, G., Hutterer, A., Berdnik, D., Mayer, B., Wirtz-Peitz, F., Gaitan, M.G., Knoblich, J.A. Cell (2005) [Pubmed]
  14. Dynein mutations impair autophagic clearance of aggregate-prone proteins. Ravikumar, B., Acevedo-Arozena, A., Imarisio, S., Berger, Z., Vacher, C., O'Kane, C.J., Brown, S.D., Rubinsztein, D.C. Nat. Genet. (2005) [Pubmed]
  15. Taking the study of cancer cell survival to a new dimension. Jacks, T., Weinberg, R.A. Cell (2002) [Pubmed]
  16. Modulation of basal nitric oxide-dependent cyclic-GMP production by ambient glucose, myo-inositol, and protein kinase C in SH-SY5Y human neuroblastoma cells. Shindo, H., Thomas, T.P., Larkin, D.D., Karihaloo, A.K., Inada, H., Onaya, T., Stevens, M.J., Greene, D.A. J. Clin. Invest. (1996) [Pubmed]
  17. Expression and localization of endothelin-1 and endothelin receptors in human meningiomas. Evidence for a role in tumoral growth. Pagotto, U., Arzberger, T., Hopfner, U., Sauer, J., Renner, U., Newton, C.J., Lange, M., Uhl, E., Weindl, A., Stalla, G.K. J. Clin. Invest. (1995) [Pubmed]
  18. Nicotine, cotinine, and anabasine inhibit aromatase in human trophoblast in vitro. Barbieri, R.L., Gochberg, J., Ryan, K.J. J. Clin. Invest. (1986) [Pubmed]
  19. Immunopotentiating reconstituted influenza virus virosome vaccine delivery system for immunization against hepatitis A. Glück, R., Mischler, R., Brantschen, S., Just, M., Althaus, B., Cryz, S.J. J. Clin. Invest. (1992) [Pubmed]
  20. Stat3 as an oncogene. Bromberg, J.F., Wrzeszczynska, M.H., Devgan, G., Zhao, Y., Pestell, R.G., Albanese, C., Darnell, J.E. Cell (1999) [Pubmed]
  21. Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell cultures. Scharf, K.D., Nover, L. Cell (1982) [Pubmed]
  22. Nuclear translocation of extradenticle requires homothorax, which encodes an extradenticle-related homeodomain protein. Rieckhof, G.E., Casares, F., Ryoo, H.D., Abu-Shaar, M., Mann, R.S. Cell (1997) [Pubmed]
  23. Differential regulation of telomerase activity and telomerase RNA during multi-stage tumorigenesis. Blasco, M.A., Rizen, M., Greider, C.W., Hanahan, D. Nat. Genet. (1996) [Pubmed]
  24. jockey, a mobile Drosophila element similar to mammalian LINEs, is transcribed from the internal promoter by RNA polymerase II. Mizrokhi, L.J., Georgieva, S.G., Ilyin, Y.V. Cell (1988) [Pubmed]
  25. Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures. Strohman, R.C., Moss, P.S., Micou-Eastwood, J., Spector, D., Przybyla, A., Paterson, B. Cell (1977) [Pubmed]
  26. Is erythropoietin the only factor which regulates late erythroid differentiation? Fagg, B. Nature (1981) [Pubmed]
  27. Expression of the putative Duchenne muscular dystrophy gene in differentiated myogenic cell cultures and in the brain. Nudel, U., Robzyk, K., Yaffe, D. Nature (1988) [Pubmed]
  28. Myoblasts transferred to the limbs of embryos are committed to specific fibre fates. DiMario, J.X., Fernyak, S.E., Stockdale, F.E. Nature (1993) [Pubmed]
  29. Injection of the cAMP-responsive element into the nucleus of Aplysia sensory neurons blocks long-term facilitation. Dash, P.K., Hochner, B., Kandel, E.R. Nature (1990) [Pubmed]
  30. Hormonal regulation of plasminogen activator mRNA production in porcine kidney cells. Nagamine, Y., Sudol, M., Reich, E. Cell (1983) [Pubmed]
  31. Hydrolase and serum treatment of normal chick embryo cells: effects on hexose transport. Hale, A.H., Weber, M.J. Cell (1975) [Pubmed]
  32. Neuronal cell killing by the envelope protein of HIV and its prevention by vasoactive intestinal peptide. Brenneman, D.E., Westbrook, G.L., Fitzgerald, S.P., Ennist, D.L., Elkins, K.L., Ruff, M.R., Pert, C.B. Nature (1988) [Pubmed]
  33. Expression of dopaminergic phenotypes in the mouse olfactory bulb induced by the calcitonin gene-related peptide. Denis-Donini, S. Nature (1989) [Pubmed]
  34. Enhancement of interferon production by poly(rI)-poly(rC) in mouse cell cultures by ascorbic acid. Siegel, B.V. Nature (1975) [Pubmed]
  35. TGF-beta1 promotes microglial amyloid-beta clearance and reduces plaque burden in transgenic mice. Wyss-Coray, T., Lin, C., Yan, F., Yu, G.Q., Rohde, M., McConlogue, L., Masliah, E., Mucke, L. Nat. Med. (2001) [Pubmed]
  36. Lipoprotein receptor-mediated induction of matrix metalloproteinase by tissue plasminogen activator. Wang, X., Lee, S.R., Arai, K., Lee, S.R., Tsuji, K., Rebeck, G.W., Lo, E.H. Nat. Med. (2003) [Pubmed]
  37. Toxin in bullous impetigo and staphylococcal scalded-skin syndrome targets desmoglein 1. Amagai, M., Matsuyoshi, N., Wang, Z.H., Andl, C., Stanley, J.R. Nat. Med. (2000) [Pubmed]
  38. EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy. Dietrich, J., Lacagnina, M., Gass, D., Richfield, E., Mayer-Pröschel, M., Noble, M., Torres, C., Pröschel, C. Nat. Med. (2005) [Pubmed]
  39. Potent inhibition of HIV-1 infectivity in macrophages and lymphocytes by a novel CCR5 antagonist. Simmons, G., Clapham, P.R., Picard, L., Offord, R.E., Rosenkilde, M.M., Schwartz, T.W., Buser, R., Wells, T.N., Proudfoot, A.E. Science (1997) [Pubmed]
  40. A circadian oscillator in cultured cells of chicken pineal gland. Deguchi, T. Nature (1979) [Pubmed]
  41. B lymphocytes secrete antigen-presenting vesicles. Raposo, G., Nijman, H.W., Stoorvogel, W., Liejendekker, R., Harding, C.V., Melief, C.J., Geuze, H.J. J. Exp. Med. (1996) [Pubmed]
  42. Corticotropin-releasing hormone is produced by rat corticotropes and modulates ACTH secretion in a paracrine/autocrine fashion. Pecori Giraldi, F., Cavagnini, F. J. Clin. Invest. (1998) [Pubmed]
  43. The effect of selective cyclooxygenase-2 inhibition in Barrett's esophagus epithelium: an in vitro study. Buttar, N.S., Wang, K.K., Anderson, M.A., Dierkhising, R.A., Pacifico, R.J., Krishnadath, K.K., Lutzke, L.S. J. Natl. Cancer Inst. (2002) [Pubmed]
  44. Immunisation with canarypox virus expressing rabies glycoprotein. Cadoz, M., Strady, A., Meignier, B., Taylor, J., Tartaglia, J., Paoletti, E., Plotkin, S. Lancet (1992) [Pubmed]
 
WikiGenes - Universities