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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Immunoblotting

 
 
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Disease relevance of Immunoblotting

 

Psychiatry related information on Immunoblotting

 

High impact information on Immunoblotting

  • The platelets were exposed to either thrombopoietin or thrombin and then lysed for analysis of tyrosine phosphorylation of platelet proteins and the expression of the proteins by means of immunoblotting [11].
  • The extent of the degradation of von Willebrand factor was assessed by electrophoresis in sodium dodecyl sulfate-agarose gels and immunoblotting [12].
  • Immunoblotting experiments, comigration on 2D gels, and 2D analysis of limit chymotryptic digests demonstrated that the 63 kd protein, present in the middle T complex in approximately equimolar ratio to the 36 kd protein, is a known regulatory subunit of the PP2A holoenzyme [13].
  • Binding of EGF to cells expressing human EGF receptor stimulated rapid tyrosine phosphorylation of phospholipase C-II (PLC-II), as revealed by immunoblotting analysis with phosphotyrosine-specific antibodies [14].
  • The levels of initiation factors eIF-2, eIF-3, eIF-4A, and eIF-4B were quantitated by immunoblotting; all are enriched in the cytoskeletal fraction relative to the soluble fraction [15].
 

Chemical compound and disease context of Immunoblotting

 

Biological context of Immunoblotting

 

Anatomical context of Immunoblotting

  • This theory is supported by immunoblotting studies, which show that DMD patients lack dystrophin in their muscle cells or that dystrophin is present at very low levels, whereas BMD patients produce a protein with reduced abundance or abnormal size [26].
  • Here, we report that the engagement of VLA-4 on T cells with specific antibodies or its ligand activates protein-tyrosine kinase (PTK) activity as detected by antiphosphotyrosine immunoblotting [27].
  • Purified eosinophils were stimulated with IL-5, and the involvement of various kinases was investigated by immunoblotting, immune complex kinase assay, and in situ denatured/renatured kinase assay [28].
  • Skin fibroblasts from 26 patients suspected of having a disorder of mitochondrial beta-oxidation were analyzed for VLCAD protein using immunoblotting, and 7 of them contained undetectable or trace levels of the enzyme [29].
  • Starvation-dependent expression of the erythroid/brain GT on the plasma membrane of these additional hepatocytes is lost within 3 h of glucose refeeding; however, by immunoblotting we show that the protein is still present [30].
 

Associations of Immunoblotting with chemical compounds

  • One of the tyrosine dephosphorylated proteins was identified as the 170-kD galactose/N-acetylgalactosamine-inhibitable lectin (Gal/GalNAc) using immunoprecipitation and immunoblotting by antibodies generated against the Gal/GalNAc lectin of the protozoan Entamoeba histolytica [31].
  • As assessed by flow cytometry and immunoblotting, C3 deposition increased on the platelet surface upon cell activation with different agonists [32].
  • Using two-dimensional gel analysis and immunoblotting techniques, it was determined that the 126,000 D glycoprotein consisted predominately of polymeric glycophorin-alpha [33].
  • After dexamethasone treatment (1 mg/kg per d for 5 d), there was no change in insulin receptor concentration in liver of rats as determined by immunoblotting with antibody to the COOH-terminus of the receptor [34].
  • In cells depleted of glutathione, intracellular AGE accumulation increased linearly with ROS generation as measured by immunoblotting and the fluorescent probe DCFH (AGE 0.258-3.531 AU* mm/5x10(4) cells, DCF 57-149 mean AU, r = .998, P < .002) [35].
 

Gene context of Immunoblotting

 

Analytical, diagnostic and therapeutic context of Immunoblotting

References

  1. Plant glutamine synthetase complements a glnA mutation in Escherichia coli. DasSarma, S., Tischer, E., Goodman, H.M. Science (1986) [Pubmed]
  2. Insights into native epitopes of proliferating cell nuclear antigen using recombinant DNA protein products. Huff, J.P., Roos, G., Peebles, C.L., Houghten, R., Sullivan, K.F., Tan, E.M. J. Exp. Med. (1990) [Pubmed]
  3. Characterization of an immunodominant Onchocerca volvulus antigen with patient sera and a monoclonal antibody. Lucius, R., Schulz-Key, H., Büttner, D.W., Kern, A., Kaltmann, B., Prod'hon, J., Seeber, F., Walter, R.D., Saxena, K.C., Diesfeld, H.J. J. Exp. Med. (1988) [Pubmed]
  4. (Auto)antibodies in human breast cancer sera against antigens associated with breast cancer cells, detected by immunoblotting. Ronai, Z., Sulitzeanu, D. J. Natl. Cancer Inst. (1986) [Pubmed]
  5. Interleukin 15-mediated induction of cytotoxic effector cells capable of eliminating Epstein-Barr virus-transformed/immortalized lymphocytes in culture. Sharif-Askari, E., Fawaz, L.M., Tran, P., Ahmad, A., Menezes, J. J. Natl. Cancer Inst. (2001) [Pubmed]
  6. An idiotype shared by monoclonal antibodies to different peptides of human myelin basic protein. Zhou, S.R., Whitaker, J.N. J. Immunol. (1990) [Pubmed]
  7. Reduced expression of amyloid precursor protein, presenilin-1 and rab3a in cortical brain regions in Alzheimer's disease. Davidsson, P., Bogdanovic, N., Lannfelt, L., Blennow, K. Dementia and geriatric cognitive disorders. (2001) [Pubmed]
  8. Assessment of IgE allergen specificity among latex-allergic health care workers: review of IgE-binding components of various latex extracts. Nielsen, P.S., Nissen, D., Skov, P.S., Cieslewicz, G., Markov, A., Babakhin, A., Zakirova, I., Gots, T., DuBuske, L.M., Sheffer, A., Nolte, H. Ann. Allergy Asthma Immunol. (2000) [Pubmed]
  9. Preparation of dopamine transporter-specific antibodies using molecular cloned genes. Lee, S.Y., Im, S.Y., Kim, K.M. Arch. Pharm. Res. (1999) [Pubmed]
  10. Anti-(U1)RNP and anti-Sm autoantibody profiles in patients with systemic rheumatic diseases: differential detection of immunoglobulin G and M by immunoblotting. Guldner, H.H., Lakomek, H.J., Bautz, F.A. Clin. Immunol. Immunopathol. (1986) [Pubmed]
  11. Impaired expression of the thrombopoietin receptor by platelets from patients with polycythemia vera. Moliterno, A.R., Hankins, W.D., Spivak, J.L. N. Engl. J. Med. (1998) [Pubmed]
  12. von Willebrand factor-cleaving protease in thrombotic thrombocytopenic purpura and the hemolytic-uremic syndrome. Furlan, M., Robles, R., Galbusera, M., Remuzzi, G., Kyrle, P.A., Brenner, B., Krause, M., Scharrer, I., Aumann, V., Mittler, U., Solenthaler, M., Lämmle, B. N. Engl. J. Med. (1998) [Pubmed]
  13. Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A. Pallas, D.C., Shahrik, L.K., Martin, B.L., Jaspers, S., Miller, T.B., Brautigan, D.L., Roberts, T.M. Cell (1990) [Pubmed]
  14. EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling. Margolis, B., Rhee, S.G., Felder, S., Mervic, M., Lyall, R., Levitzki, A., Ullrich, A., Zilberstein, A., Schlessinger, J. Cell (1989) [Pubmed]
  15. Translational initiation factor and ribosome association with the cytoskeletal framework fraction from HeLa cells. Howe, J.G., Hershey, J.W. Cell (1984) [Pubmed]
  16. Primary structure and nuclear localization of a murine homeodomain protein. Kessel, M., Schulze, F., Fibi, M., Gruss, P. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  17. Analysis of elevated fibrin(ogen) degradation product levels in patients with liver disease. vanDeWater, L., Carr, J.M., Aronson, D., McDonagh, J. Blood (1986) [Pubmed]
  18. Platelets from spontaneously hypertensive rats exhibit decreased expression of inhibitory guanine nucleotide regulatory protein. Relation with adenylyl cyclase activity. Anand-Srivastava, M.B. Circ. Res. (1993) [Pubmed]
  19. Characterization of the molecular forms of fibronectin in fulminant hepatic failure. Almasio, P.L., Hughes, R.D., Williams, R. Hepatology (1986) [Pubmed]
  20. HBcAg expressed on the surface of circulating Dane particles in patients with hepatitis B virus infection without evidence of anti-HBc formation. Möller, B., Hopf, U., Stemerowicz, R., Henze, G., Gelderblom, H. Hepatology (1989) [Pubmed]
  21. Progressive resistance to apoptosis in a cell lineage model of human proliferative breast disease. Starcevic, S.L., Elferink, C., Novak, R.F. J. Natl. Cancer Inst. (2001) [Pubmed]
  22. A single nucleotide substitution introduces a premature termination codon into the androgen receptor gene of a patient with receptor-negative androgen resistance. Marcelli, M., Tilley, W.D., Wilson, C.M., Wilson, J.D., Griffin, J.E., McPhaul, M.J. J. Clin. Invest. (1990) [Pubmed]
  23. Hypotension and reduced nitric oxide-elicited vasorelaxation in transgenic mice overexpressing endothelial nitric oxide synthase. Ohashi, Y., Kawashima, S., Hirata, K., Yamashita, T., Ishida, T., Inoue, N., Sakoda, T., Kurihara, H., Yazaki, Y., Yokoyama, M. J. Clin. Invest. (1998) [Pubmed]
  24. A comparative analysis by SAGE of gene expression profiles of Barrett's esophagus, normal squamous esophagus, and gastric cardia. van Baal, J.W., Milano, F., Rygiel, A.M., Bergman, J.J., Rosmolen, W.D., van Deventer, S.J., Wang, K.K., Peppelenbosch, M.P., Krishnadath, K.K. Gastroenterology (2005) [Pubmed]
  25. Molecular mechanism of interferon alfa-mediated growth inhibition in human neuroendocrine tumor cells. Detjen, K.M., Welzel, M., Farwig, K., Brembeck, F.H., Kaiser, A., Riecken, E.O., Wiedenmann, B., Rosewicz, S. Gastroenterology (2000) [Pubmed]
  26. Very mild muscular dystrophy associated with the deletion of 46% of dystrophin. England, S.B., Nicholson, L.V., Johnson, M.A., Forrest, S.M., Love, D.R., Zubrzycka-Gaarn, E.E., Bulman, D.E., Harris, J.B., Davies, K.E. Nature (1990) [Pubmed]
  27. Ligation of VLA-4 on T cells stimulates tyrosine phosphorylation of a 105-kD protein. Nojima, Y., Rothstein, D.M., Sugita, K., Schlossman, S.F., Morimoto, C. J. Exp. Med. (1992) [Pubmed]
  28. The intracellular signal transduction mechanism of interleukin 5 in eosinophils: the involvement of lyn tyrosine kinase and the Ras-Raf-1-MEK-microtubule-associated protein kinase pathway. Pazdrak, K., Schreiber, D., Forsythe, P., Justement, L., Alam, R. J. Exp. Med. (1995) [Pubmed]
  29. Purification of human very-long-chain acyl-coenzyme A dehydrogenase and characterization of its deficiency in seven patients. Aoyama, T., Souri, M., Ushikubo, S., Kamijo, T., Yamaguchi, S., Kelley, R.I., Rhead, W.J., Uetake, K., Tanaka, K., Hashimoto, T. J. Clin. Invest. (1995) [Pubmed]
  30. Restricted expression of the erythroid/brain glucose transporter isoform to perivenous hepatocytes in rats. Modulation by glucose. Tal, M., Schneider, D.L., Thorens, B., Lodish, H.F. J. Clin. Invest. (1990) [Pubmed]
  31. Identification of a Gal/GalNAc lectin in the protozoan Hartmannella vermiformis as a potential receptor for attachment and invasion by the Legionnaires' disease bacterium. Venkataraman, C., Haack, B.J., Bondada, S., Abu Kwaik, Y. J. Exp. Med. (1997) [Pubmed]
  32. Platelet activation leads to activation and propagation of the complement system. Del Conde, I., Crúz, M.A., Zhang, H., López, J.A., Afshar-Kharghan, V. J. Exp. Med. (2005) [Pubmed]
  33. Increased efficiency of binding of nascent C3b to the erythrocytes of chronic cold agglutinin disease. Parker, C.J., Soldato, C.M., Telen, M.J. J. Clin. Invest. (1984) [Pubmed]
  34. Modulation of insulin receptor, insulin receptor substrate-1, and phosphatidylinositol 3-kinase in liver and muscle of dexamethasone-treated rats. Saad, M.J., Folli, F., Kahn, J.A., Kahn, C.R. J. Clin. Invest. (1993) [Pubmed]
  35. BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells. Giardino, I., Edelstein, D., Brownlee, M. J. Clin. Invest. (1996) [Pubmed]
  36. Decreased mutation rate for cellular resistance to doxorubicin and suppression of mdr1 gene activation by the cyclosporin PSC 833. Beketic-Oreskovic, L., Durán, G.E., Chen, G., Dumontet, C., Sikic, B.I. J. Natl. Cancer Inst. (1995) [Pubmed]
  37. In vivo proteolysis of serum insulin-like growth factor (IGF) binding protein-3 results in increased availability of IGF to target cells. Blat, C., Villaudy, J., Binoux, M. J. Clin. Invest. (1994) [Pubmed]
  38. Keratinocyte growth factor and hepatocyte growth factor/scatter factor are heparin-binding growth factors for alveolar type II cells in fibroblast-conditioned medium. Panos, R.J., Rubin, J.S., Csaky, K.G., Aaronson, S.A., Mason, R.J. J. Clin. Invest. (1993) [Pubmed]
  39. Association of amino acid sequences in the HLA-DQB1 first domain with antitopoisomerase I autoantibody response in scleroderma (progressive systemic sclerosis). Reveille, J.D., Durban, E., MacLeod-St Clair, M.J., Goldstein, R., Moreda, R., Altman, R.D., Arnett, F.C. J. Clin. Invest. (1992) [Pubmed]
  40. Fractalkine-mediated signals regulate cell-survival and immune-modulatory responses in intestinal epithelial cells. Brand, S., Sakaguchi, T., Gu, X., Colgan, S.P., Reinecker, H.C. Gastroenterology (2002) [Pubmed]
  41. Shiverer peripheral myelin contains P2. Winter, J. Nature (1982) [Pubmed]
  42. Subunit composition of plasma von Willebrand factor. Cleavage is present in normal individuals, increased in IIA and IIB von Willebrand disease, but minimal in variants with aberrant structure of individual oligomers (types IIC, IID, and IIE). Zimmerman, T.S., Dent, J.A., Ruggeri, Z.M., Nannini, L.H. J. Clin. Invest. (1986) [Pubmed]
  43. Autoantibodies against nucleoporin p62 constitute a novel marker of primary biliary cirrhosis. Wesierska-Gadek, J., Hohenuer, H., Hitchman, E., Penner, E. Gastroenterology (1996) [Pubmed]
  44. Myomesin and M-protein: expression of two M-band proteins in pectoral muscle and heart during development. Grove, B.K., Cerny, L., Perriard, J.C., Eppenberger, H.M. J. Cell Biol. (1985) [Pubmed]
  45. Calcium-dependent association of a protein complex with the lymphocyte plasma membrane: probable identity with calmodulin-calcineurin. Chantler, P.D. J. Cell Biol. (1985) [Pubmed]
 
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