The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Transfection


High impact information on Transfection


Chemical compound and disease context of Transfection


Biological context of Transfection

  • PIF3 localized to the nucleus in transient transfection experiments, indicating a potential role in controlling gene expression [16].
  • The structure of the cloned complementary DNA was analyzed by nucleotide sequencing, and its function was assessed on the basis of its ability to restore to normal the abnormal phenotype of a PIG-A-deficient cell line after transfection [17].
  • Mutagenesis of an AP2 DNA-binding site within a p21 promoter-luciferase reporter inhibited its activation by either AP2 transfection or TPA stimulation [18].
  • Even though the R659Q protein is expressed, these cells act as if they were TAP deficient by peptide binding and antigen presentation studies, which are restored after transfection of a functional TAP1 allele [19].
  • The NFB MyoD gene is silent, but can be activated upon transfection of a long terminal region-controlled chicken MyoD cDNA, resulting in myogenesis [20].

Anatomical context of Transfection


Associations of Transfection with chemical compounds

  • Transfection of the steroid-inducible LTR-C3 gene into unresponsive S115 mouse mammary tumor cells results in full inducibility of that gene with both androgen and glucocorticoid [26].
  • Transfection of THP-1 cells demonstrates that the Asp299Gly mutation (but not the Thr399Ile mutation) interrupts TLR4-mediated LPS signalling [27].
  • The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells [28].
  • Analysis of 347 plaques obtained after transfection of this modified DNA indicated that mispairs were corrected in 343 cases (99%), revealing 314 repair events in favor of guanine (90%) and 29 in favor of thymine (8%) [29].
  • We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colonies [30].

Gene context of Transfection

  • Analysis of tissue samples and transfection of CNTF minigenes into cultured cells demonstrates that the mutated allele expresses only the mutated mRNA species [31].
  • Transfection with the wild-type RFXAP gene restored the expression of MHC class II molecules in the patients' cells [32].
  • Transient transfection experiments using wild-type or inactivated c-Abl show that both induce expression of p21, an effector of p53, but only wild-type c-Abl downregulates the activity of the cyclin-dependent kinase Cdk2 and causes growth arrest [33].
  • These non-permissive cells are successfully transduced by AAV vectors after stable transfections with cDNAs encoding the murine HSPG and the human FGFR1 [34].
  • Transient transfection of Hox/HOXA5 activated the p53 promoter [35].

Analytical, diagnostic and therapeutic context of Transfection


  1. Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies. Drebin, J.A., Link, V.C., Stern, D.F., Weinberg, R.A., Greene, M.I. Cell (1985) [Pubmed]
  2. Transformation mediated by the SV40 T antigens: separation of the overlapping SV40 early genes with a retroviral vector. Kriegler, M., Perez, C.F., Hardy, C., Botchan, M. Cell (1984) [Pubmed]
  3. DNA methylation affects the formation of active chromatin. Keshet, I., Lieman-Hurwitz, J., Cedar, H. Cell (1986) [Pubmed]
  4. Altered gene products are associated with activation of cellular rasK genes in human lung and colon carcinomas. Der, C.J., Cooper, G.M. Cell (1983) [Pubmed]
  5. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Andersson, P., Goldfarb, M.P., Weinberg, R.A. Cell (1979) [Pubmed]
  6. The male-specific histocompatibility antigen, H-Y: a history of transplantation, immune response genes, sex determination and expression cloning. Simpson, E., Scott, D., Chandler, P. Annu. Rev. Immunol. (1997) [Pubmed]
  7. Nonsense-mediated decay microarray analysis identifies mutations of EPHB2 in human prostate cancer. Huusko, P., Ponciano-Jackson, D., Wolf, M., Kiefer, J.A., Azorsa, D.O., Tuzmen, S., Weaver, D., Robbins, C., Moses, T., Allinen, M., Hautaniemi, S., Chen, Y., Elkahloun, A., Basik, M., Bova, G.S., Bubendorf, L., Lugli, A., Sauter, G., Schleutker, J., Ozcelik, H., Elowe, S., Pawson, T., Trent, J.M., Carpten, J.D., Kallioniemi, O.P., Mousses, S. Nat. Genet. (2004) [Pubmed]
  8. Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Bamforth, S.D., Bragança, J., Farthing, C.R., Schneider, J.E., Broadbent, C., Michell, A.C., Clarke, K., Neubauer, S., Norris, D., Brown, N.A., Anderson, R.H., Bhattacharya, S. Nat. Genet. (2004) [Pubmed]
  9. Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages. Gutierrez, M.G., Master, S.S., Singh, S.B., Taylor, G.A., Colombo, M.I., Deretic, V. Cell (2004) [Pubmed]
  10. A common polymorphism of the growth hormone receptor is associated with increased responsiveness to growth hormone. Dos Santos, C., Essioux, L., Teinturier, C., Tauber, M., Goffin, V., Bougnères, P. Nat. Genet. (2004) [Pubmed]
  11. Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity. Kasid, A., Lippman, M.E., Papageorge, A.G., Lowy, D.R., Gelmann, E.P. Science (1985) [Pubmed]
  12. Polymorphism in the human apolipoprotein A-I gene promoter region. Association of the minor allele with decreased production rate in vivo and promoter activity in vitro. Smith, J.D., Brinton, E.A., Breslow, J.L. J. Clin. Invest. (1992) [Pubmed]
  13. Hemidesmosome formation is initiated by the beta4 integrin subunit, requires complex formation of beta4 and HD1/plectin, and involves a direct interaction between beta4 and the bullous pemphigoid antigen 180. Schaapveld, R.Q., Borradori, L., Geerts, D., van Leusden, M.R., Kuikman, I., Nievers, M.G., Niessen, C.M., Steenbergen, R.D., Snijders, P.J., Sonnenberg, A. J. Cell Biol. (1998) [Pubmed]
  14. Syndecan-1 expressed in Schwann cells causes morphological transformation and cytoskeletal reorganization and associates with actin during cell spreading. Carey, D.J., Stahl, R.C., Cizmeci-Smith, G., Asundi, V.K. J. Cell Biol. (1994) [Pubmed]
  15. A progesterone responsive element maps to the far upstream steroid dependent DNase hypersensitive site of chicken lysozyme chromatin. Hecht, A., Berkenstam, A., Strömstedt, P.E., Gustafsson, J.A., Sippel, A.E. EMBO J. (1988) [Pubmed]
  16. PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein. Ni, M., Tepperman, J.M., Quail, P.H. Cell (1998) [Pubmed]
  17. Abnormalities of PIG-A transcripts in granulocytes from patients with paroxysmal nocturnal hemoglobinuria. Miyata, T., Yamada, N., Iida, Y., Nishimura, J., Takeda, J., Kitani, T., Kinoshita, T. N. Engl. J. Med. (1994) [Pubmed]
  18. AP2 inhibits cancer cell growth and activates p21WAF1/CIP1 expression. Zeng, Y.X., Somasundaram, K., el-Deiry, W.S. Nat. Genet. (1997) [Pubmed]
  19. A functionally defective allele of TAP1 results in loss of MHC class I antigen presentation in a human lung cancer. Chen, H.L., Gabrilovich, D., Tampé, R., Girgis, K.R., Nadaf, S., Carbone, D.P. Nat. Genet. (1996) [Pubmed]
  20. Negative control of the helix-loop-helix family of myogenic regulators in the NFB mutant. Peterson, C.A., Gordon, H., Hall, Z.W., Paterson, B.M., Blau, H.M. Cell (1990) [Pubmed]
  21. Myogenic lineage determination and differentiation: evidence for a regulatory gene pathway. Pinney, D.F., Pearson-White, S.H., Konieczny, S.F., Latham, K.E., Emerson, C.P. Cell (1988) [Pubmed]
  22. Identification of a phosphoprotein specifically induced by the transforming DNA of rat neuroblastomas. Padhy, L.C., Shih, C., Cowing, D., Finkelstein, R., Weinberg, R.A. Cell (1982) [Pubmed]
  23. Expression of preprosomatostatin in heterologous cells: biosynthesis, posttranslational processing, and secretion of mature somatostatin. Warren, T.G., Shields, D. Cell (1984) [Pubmed]
  24. The transfection of epidermal keratin genes into fibroblasts and simple epithelial cells: evidence for inducing a type I keratin by a type II gene. Giudice, G.J., Fuchs, E. Cell (1987) [Pubmed]
  25. Peroxisome assembly factor-2, a putative ATPase cloned by functional complementation on a peroxisome-deficient mammalian cell mutant. Tsukamoto, T., Miura, S., Nakai, T., Yokota, S., Shimozawa, N., Suzuki, Y., Orii, T., Fujiki, Y., Sakai, F., Bogaki, A., Yasumo, H., Osumi, T. Nat. Genet. (1995) [Pubmed]
  26. Progression to steroid insensitivity can occur irrespective of the presence of functional steroid receptors. Darbre, P.D., King, R.J. Cell (1987) [Pubmed]
  27. TLR4 mutations are associated with endotoxin hyporesponsiveness in humans. Arbour, N.C., Lorenz, E., Schutte, B.C., Zabner, J., Kline, J.N., Jones, M., Frees, K., Watt, J.L., Schwartz, D.A. Nat. Genet. (2000) [Pubmed]
  28. The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells. Mayo, K.E., Warren, R., Palmiter, R.D. Cell (1982) [Pubmed]
  29. A specific mismatch repair event protects mammalian cells from loss of 5-methylcytosine. Brown, T.C., Jiricny, J. Cell (1987) [Pubmed]
  30. Transfection of a DNA locus that mediates the conversion of 10T1/2 fibroblasts to myoblasts. Lassar, A.B., Paterson, B.M., Weintraub, H. Cell (1986) [Pubmed]
  31. A null mutation in the human CNTF gene is not causally related to neurological diseases. Takahashi, R., Yokoji, H., Misawa, H., Hayashi, M., Hu, J., Deguchi, T. Nat. Genet. (1994) [Pubmed]
  32. Mutation of RFXAP, a regulator of MHC class II genes, in primary MHC class II deficiency. Villard, J., Lisowska-Grospierre, B., van den Elsen, P., Fischer, A., Reith, W., Mach, B. N. Engl. J. Med. (1997) [Pubmed]
  33. Role for c-Abl tyrosine kinase in growth arrest response to DNA damage. Yuan, Z.M., Huang, Y., Whang, Y., Sawyers, C., Weichselbaum, R., Kharbanda, S., Kufe, D. Nature (1996) [Pubmed]
  34. Human fibroblast growth factor receptor 1 is a co-receptor for infection by adeno-associated virus 2. Qing, K., Mah, C., Hansen, J., Zhou, S., Dwarki, V., Srivastava, A. Nat. Med. (1999) [Pubmed]
  35. Compromised HOXA5 function can limit p53 expression in human breast tumours. Raman, V., Martensen, S.A., Reisman, D., Evron, E., Odenwald, W.F., Jaffee, E., Marks, J., Sukumar, S. Nature (2000) [Pubmed]
  36. The genetic basis of epidermolytic hyperkeratosis: a disorder of differentiation-specific epidermal keratin genes. Cheng, J., Syder, A.J., Yu, Q.C., Letai, A., Paller, A.S., Fuchs, E. Cell (1992) [Pubmed]
  37. Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. Nunes, M.C., Roy, N.S., Keyoung, H.M., Goodman, R.R., McKhann, G., Jiang, L., Kang, J., Nedergaard, M., Goldman, S.A. Nat. Med. (2003) [Pubmed]
  38. Microtubule organization and dynamics dependent on microtubule-associated proteins. Hirokawa, N. Curr. Opin. Cell Biol. (1994) [Pubmed]
  39. PML suppresses oncogenic transformation of NIH/3T3 cells by activated neu. Liu, J.H., Mu, Z.M., Chang, K.S. J. Exp. Med. (1995) [Pubmed]
  40. Immunotherapy of a murine tumor with interleukin 2. Increased sensitivity after MHC class I gene transfection. Weber, J.S., Jay, G., Tanaka, K., Rosenberg, S.A. J. Exp. Med. (1987) [Pubmed]
WikiGenes - Universities