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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Transfection

 
 
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Disease relevance of Transfection

 

High impact information on Transfection

 

Chemical compound and disease context of Transfection

 

Biological context of Transfection

  • PIF3 localized to the nucleus in transient transfection experiments, indicating a potential role in controlling gene expression [16].
  • The structure of the cloned complementary DNA was analyzed by nucleotide sequencing, and its function was assessed on the basis of its ability to restore to normal the abnormal phenotype of a PIG-A-deficient cell line after transfection [17].
  • Mutagenesis of an AP2 DNA-binding site within a p21 promoter-luciferase reporter inhibited its activation by either AP2 transfection or TPA stimulation [18].
  • Even though the R659Q protein is expressed, these cells act as if they were TAP deficient by peptide binding and antigen presentation studies, which are restored after transfection of a functional TAP1 allele [19].
  • The NFB MyoD gene is silent, but can be activated upon transfection of a long terminal region-controlled chicken MyoD cDNA, resulting in myogenesis [20].
 

Anatomical context of Transfection

 

Associations of Transfection with chemical compounds

  • Transfection of the steroid-inducible LTR-C3 gene into unresponsive S115 mouse mammary tumor cells results in full inducibility of that gene with both androgen and glucocorticoid [26].
  • Transfection of THP-1 cells demonstrates that the Asp299Gly mutation (but not the Thr399Ile mutation) interrupts TLR4-mediated LPS signalling [27].
  • The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells [28].
  • Analysis of 347 plaques obtained after transfection of this modified DNA indicated that mispairs were corrected in 343 cases (99%), revealing 314 repair events in favor of guanine (90%) and 29 in favor of thymine (8%) [29].
  • We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colonies [30].
 

Gene context of Transfection

  • Analysis of tissue samples and transfection of CNTF minigenes into cultured cells demonstrates that the mutated allele expresses only the mutated mRNA species [31].
  • Transfection with the wild-type RFXAP gene restored the expression of MHC class II molecules in the patients' cells [32].
  • Transient transfection experiments using wild-type or inactivated c-Abl show that both induce expression of p21, an effector of p53, but only wild-type c-Abl downregulates the activity of the cyclin-dependent kinase Cdk2 and causes growth arrest [33].
  • These non-permissive cells are successfully transduced by AAV vectors after stable transfections with cDNAs encoding the murine HSPG and the human FGFR1 [34].
  • Transient transfection of Hox/HOXA5 activated the p53 promoter [35].
 

Analytical, diagnostic and therapeutic context of Transfection

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