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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Fluorescence

 
 
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Disease relevance of Fluorescence

 

Psychiatry related information on Fluorescence

 

High impact information on Fluorescence

  • We used total internal reflection fluorescence microscopy to observe directly individual actin filament polymerization in the presence of two mammalian formins (mDia1 and mDia2) and two yeast formins (Bni1p and Cdc12p) [11].
  • Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma(70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase [12].
  • After injecting fluorescence-labeled tubulin into the axons, we monitored the movement of fluorescence by confocal laser scanning microscopy and fluorescence correlation spectroscopy [13].
  • RESULTS: The expression of annexin II, as detected by a fluorescein-tagged antibody, was greater on leukemic cells from patients with APL than on other types of leukemic cells (mean fluorescence intensity, 6.9 and 2.9, respectively; P<0.01) [14].
  • GFP-Hras colocalized with GFP-Nras, but GFP-Kras4B revealed less Golgi and no vesicular fluorescence [15].
 

Chemical compound and disease context of Fluorescence

 

Biological context of Fluorescence

 

Anatomical context of Fluorescence

  • Erythrocyte ghosts containing a known number of molecules of purified fragment A of diphtheria toxin with a constant amount of FITC-BSA as a fluorescence marker were prepared by dialyzing a mixture of erythrocytes and these substances against hypotonic solution [26].
  • Living cultured fibroblasts were microinjected with rhodamine-labeled smooth muscle alpha-actinin and visualized by video-intensified fluorescence microscopy [27].
  • We have used fluorescence microscopy and uptake of radioactive dye to study MC 540 staining of peripheral blood leukocytes from 80 leukemic and 34 normal individuals; leukemic leukocytes stain, whereas normal leukcytes do not [28].
  • Extraction of soluble lumenal proteins from microsomes and reconstitution with purified proteins demonstrate, by fluorescence collisional quenching, that BiP seals the lumenal end of this pore [29].
  • Dyes were positioned at various locations across the entire bilayer and inside the ribosome, and in each case the probes were in an aqueous milieu, as shown both by their fluorescence lifetimes and by collisional quenching of their fluorescence by iodide ions introduced into the ER lumen [30].
 

Associations of Fluorescence with chemical compounds

 

Gene context of Fluorescence

  • A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells [36].
  • Despite the altered distribution of Rap1p in rlf2 mutant cells, fluorescence in situ hybridization to subtelomeric repeats shows that the distribution of telomeric DNA is similar in wild-type and mutant cells [37].
  • Taking advantage of the natural fluorescence imparted to yeast spores by the presence of a dityrosine-containing macromolecule in the spore wall, we identified and cloned two genes, termed DIT1 and DIT2, which are required for spore wall maturation [38].
  • We developed quantitative time-lapse fluorescence microscopy on a multicell-cycle timescale, for following expression of unstable GFP under control of the G1 cyclin CLN2 promoter [39].
  • GM-CSF (800 U/ml) (mean fluorescence channel 2.54 +/- 0.33 times the control, p less than 0.001) and IFN-gamma (100 U/ml) (5.14 +/- 0.60, p less than 0.001) were the most potent inducers of HLA-DR [40].
 

Analytical, diagnostic and therapeutic context of Fluorescence

  • In indirect immunofluorescence, these two views have revealed that desmin is present at the periphery of each Z disc, forming a network of proteinaceous collars within the Z plane. alpha-Actinin is localized within each disc, giving a face-on fluorescence pattern that is complementary to that of desmin [41].
  • Nearly all techniques sensitive to dynamics have given evidence for intramolecular mobility in proteins: NMR, ESR, Raman spectroscopy, fluorescence quenching, Mössbauer spectroscopy, neutron scattering, measurements of elastic constants and hydrogen-deuterium exchange [42].
  • A novel chemical sensor has been developed in which the polymer ethylene-vinyl acetate is used as a controlled-release system to deliver reagents to the sensing region of an optical fiber for a homogeneous competitive immunoassay based on fluorescence energy transfer [43].
  • Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering perfusion pressure from 55 to 10 torr caused a heterogeneous increase in NADH fluorescence, reflecting the heterogeneous oxygen delivery at this low pressure [44].
  • In further phenotypic studies, we analyzed surface molecules of PP and spleen DCs by flow cytometry and found that these cells had similar fluorescence profiles when stained with N418, NLDC-145, and 33D1 DC-reactive antibodies, and antibodies to the costimulatory molecules B7-1 (1G10) and B7-2 (GL1) [45].

References

  1. Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Ha, T., Rasnik, I., Cheng, W., Babcock, H.P., Gauss, G.H., Lohman, T.M., Chu, S. Nature (2002) [Pubmed]
  2. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Matayoshi, E.D., Wang, G.T., Krafft, G.A., Erickson, J. Science (1990) [Pubmed]
  3. Regions of cerebral ischemia located by pyridine nucleotide fluorescence. Welsh, F.A., O'Connor, M.J., Langfitt, T.W. Science (1977) [Pubmed]
  4. Serum protein-bound carbohydrates for following the course of disease in patients with metastatic breast carcinoma. Waalkes, T.P., Mrochek, J.E., Dinsmore, S.R., Tormey, D.C. J. Natl. Cancer Inst. (1978) [Pubmed]
  5. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. Pollack, M.S., Heagney, S.D., Livingston, P.O., Fogh, J. J. Natl. Cancer Inst. (1981) [Pubmed]
  6. Family study of platelet membrane fluidity in Alzheimer's disease. Zubenko, G.S., Wusylko, M., Cohen, B.M., Boller, F., Teply, I. Science (1987) [Pubmed]
  7. The temporal sequence of events in the activation of phospholipase A2 by lipid vesicles. Studies with the monomeric enzyme from Agkistrodon piscivorus piscivorus. Bell, J.D., Biltonen, R.L. J. Biol. Chem. (1989) [Pubmed]
  8. Inhibitor-induced changes in the intrinsic fluorescence of human cyclooxygenase-2. Houtzager, V., Ouellet, M., Falgueyret, J.P., Passmore, L.A., Bayly, C., Percival, M.D. Biochemistry (1996) [Pubmed]
  9. Applicability of the induced-fit model to glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. Study of the binding of oxidized nicotinamide adenine dinucleotide and nicotinamide 8-bromoadenine dinucleotide. Branlant, G., Eiler, B., Biellmann, J.F., Lutz, H.P., Luisi, P.L. Biochemistry (1983) [Pubmed]
  10. A fluorescence decay time study of tryptophan in isolated hemoglobin subunits. Albani, J., Alpert, B., Krajcarski, D.T., Szabo, A.G. FEBS Lett. (1985) [Pubmed]
  11. Control of the assembly of ATP- and ADP-actin by formins and profilin. Kovar, D.R., Harris, E.S., Mahaffy, R., Higgs, H.N., Pollard, T.D. Cell (2006) [Pubmed]
  12. Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA. Mukhopadhyay, J., Kapanidis, A.N., Mekler, V., Kortkhonjia, E., Ebright, Y.W., Ebright, R.H. Cell (2001) [Pubmed]
  13. Oligomeric tubulin in large transporting complex is transported via kinesin in squid giant axons. Terada, S., Kinjo, M., Hirokawa, N. Cell (2000) [Pubmed]
  14. Annexin II and bleeding in acute promyelocytic leukemia. Menell, J.S., Cesarman, G.M., Jacovina, A.T., McLaughlin, M.A., Lev, E.A., Hajjar, K.A. N. Engl. J. Med. (1999) [Pubmed]
  15. Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi. Choy, E., Chiu, V.K., Silletti, J., Feoktistov, M., Morimoto, T., Michaelson, D., Ivanov, I.E., Philips, M.R. Cell (1999) [Pubmed]
  16. Maillard reaction products and their relation to complications in insulin-dependent diabetes mellitus. McCance, D.R., Dyer, D.G., Dunn, J.A., Bailie, K.E., Thorpe, S.R., Baynes, J.W., Lyons, T.J. J. Clin. Invest. (1993) [Pubmed]
  17. Tyrosine phosphorylation at a site highly conserved in the L1 family of cell adhesion molecules abolishes ankyrin binding and increases lateral mobility of neurofascin. Garver, T.D., Ren, Q., Tuvia, S., Bennett, V. J. Cell Biol. (1997) [Pubmed]
  18. Surface expression of viral glycoproteins is polarized in epithelial cells infected with recombinant vaccinia viral vectors. Stephens, E.B., Compans, R.W., Earl, P., Moss, B. EMBO J. (1986) [Pubmed]
  19. The binding of 6-demethylchlortetracycline to 70S, 50S and 30S ribosomal particles: a quantitative study by fluorescence anisotropy. Epe, B., Woolley, P. EMBO J. (1984) [Pubmed]
  20. Periportal and pericentral pyridine nucleotide fluorescence from the surface of the perfused liver: evaluation of the hypothesis that chronic treatment with ethanol produces pericentral hypoxia. Ji, S., Lemasters, J.J., Christenson, V., Thurman, R.G. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  21. Characterization of Giemsa dark- and light-band DNA. Holmquist, G., Gray, M., Porter, T., Jordan, J. Cell (1982) [Pubmed]
  22. Analysis of transferrin recycling in mitotic and interphase HeLa cells by quantitative fluorescence microscopy. Sager, P.R., Brown, P.A., Berlin, R.D. Cell (1984) [Pubmed]
  23. Chromosomal localization of seven PAX genes and cloning of a novel family member, PAX-9. Stapleton, P., Weith, A., Urbánek, P., Kozmik, Z., Busslinger, M. Nat. Genet. (1993) [Pubmed]
  24. Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells. Bostock, C.J., Clark, E.M. Cell (1980) [Pubmed]
  25. Identification of fetal DNA and cells in skin lesions from women with systemic sclerosis. Artlett, C.M., Smith, J.B., Jimenez, S.A. N. Engl. J. Med. (1998) [Pubmed]
  26. One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell. Yamaizumi, M., Mekada, E., Uchida, T., Okada, Y. Cell (1978) [Pubmed]
  27. Stress fiber sarcomeres of fibroblasts are contractile. Kreis, T.E., Birchmeier, W. Cell (1980) [Pubmed]
  28. Merocyanine 540 as a fluorescent probe of membranes: selective staining of leukemic and immature hemopoietic cells. Valinsky, J.E., Easton, T.G., Reich, E. Cell (1978) [Pubmed]
  29. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation. Hamman, B.D., Hendershot, L.M., Johnson, A.E. Cell (1998) [Pubmed]
  30. Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore. Crowley, K.S., Liao, S., Worrell, V.E., Reinhart, G.D., Johnson, A.E. Cell (1994) [Pubmed]
  31. GroEL-mediated protein folding proceeds by multiple rounds of binding and release of nonnative forms. Weissman, J.S., Kashi, Y., Fenton, W.A., Horwich, A.L. Cell (1994) [Pubmed]
  32. Surface functions during Mitosis I: phagocytosis, pinocytosis and mobility of surface-bound Con A. Berlin, R.D., Oliver, J.M., Walter, R.J. Cell (1978) [Pubmed]
  33. Incorporation of exogenous pyrene-labeled histone into Physarum chromatin: a system for studying changes in nucleosomes assembled in vivo. Prior, C.P., Cantor, C.R., Johnson, E.M., Allfrey, V.G. Cell (1980) [Pubmed]
  34. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product. Rohrschneider, L.R. Cell (1979) [Pubmed]
  35. The energized membrane and cellular autolysis in Bacillus subtilis. Jolliffe, L.K., Doyle, R.J., Streips, U.N. Cell (1981) [Pubmed]
  36. Localized Rac activation dynamics visualized in living cells. Kraynov, V.S., Chamberlain, C., Bokoch, G.M., Schwartz, M.A., Slabaugh, S., Hahn, K.M. Science (2000) [Pubmed]
  37. RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Enomoto, S., McCune-Zierath, P.D., Gerami-Nejad, M., Sanders, M.A., Berman, J. Genes Dev. (1997) [Pubmed]
  38. Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae. Briza, P., Breitenbach, M., Ellinger, A., Segall, J. Genes Dev. (1990) [Pubmed]
  39. Coherence and timing of cell cycle start examined at single-cell resolution. Bean, J.M., Siggia, E.D., Cross, F.R. Mol. Cell (2006) [Pubmed]
  40. Cytokines in chronic inflammatory arthritis. IV. Granulocyte/macrophage colony-stimulating factor-mediated induction of class II MHC antigen on human monocytes: a possible role in rheumatoid arthritis. Alvaro-Gracia, J.M., Zvaifler, N.J., Firestein, G.S. J. Exp. Med. (1989) [Pubmed]
  41. The existence of an insoluble Z disc scaffold in chicken skeletal muscle. Granger, B.L., Lazarides, E. Cell (1978) [Pubmed]
  42. Molecular dynamics studied by analysis of the X-ray diffuse scattering from lysozyme crystals. Doucet, J., Benoit, J.P. Nature (1987) [Pubmed]
  43. Chemical sensors based on controlled-release polymer systems. Barnard, S.M., Walt, D.R. Science (1991) [Pubmed]
  44. Ischemic areas in perfused rat hearts: measurement by NADH fluorescence photography. Barlow, C.H., Chance, B. Science (1976) [Pubmed]
  45. Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch. Kelsall, B.L., Strober, W. J. Exp. Med. (1996) [Pubmed]
 
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