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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Hemadsorption


High impact information on Hemadsorption

  • Cells fixed with PFA but not with GA exhibited hemadsorption with green monkey cells [6].
  • Immune adherence, protein A, and C3-mixed hemadsorption assays detected reactivity in a high percentage of patients (80-100%), whereas mixed hemadsorption assays were negative with sera from all but one patient [7].
  • Quantitative absorption experiments in the mixed hemadsorption assay determined that the expression of MAA and beta 2m on treated cells was enhanced at 10(2)-10(5) U IFN/ml, twofold to fivefold for MAA and fivefold to twelvefold for beta 2m [8].
  • After short-term culture in vitro, however, cells from all three types of tissue were only weakly agglutinated by Con A, as measured by both suspension and hemadsorption assays [9].
  • Positive reactions ranged from 12 to 32% in mixed hemadsorption for anitbody detection and from 10 to 34% in mixed hemadsorption for complement component 3 detection [10].

Biological context of Hemadsorption


Anatomical context of Hemadsorption

  • Cells expressing C28a HN did not bind erythrocytes at 4 degrees C unless pretreated with neuraminidase, but no such pretreatment was required for hemadsorption activity (HAD) at 22 or 37 degrees C. HAD was blocked by 4-GU-DANA, attesting to the ability of this compound to inhibit HN's receptor-binding activity [15].
  • The former downregulate CD46, the putative cellular receptor of MV, are positive for hemadsorption, and are fusogenic in HeLa cells, whereas the latter are negative for these phenotypic markers [1].
  • Focal syncytia were formed containing measles antigen and accumulations of nucleocapsid-like structures with no detectable production of transmissible infectious virus or positive hemadsorption [16].
  • The results of an in vitro mixed hemadsorption (MHA) assay predicted the success of in vivo tumor localization using a radioiodinated, monoclonal, IgG1 antibody (A2) with reactivity to the human bladder carcinoma cell line RT4 [17].

Associations of Hemadsorption with chemical compounds


Gene context of Hemadsorption

  • Moreover, in the hemagglutinin gene of a strain showing strongly reduced hemadsorption, 20 clustered A to G mutations, resulting in 16 amino acid changes, were detected [22].
  • Sera from all mice were screened for reactivity to Meth A by complement-dependent cytotoxicity, protein A, and mixed hemadsorption assays [23].
  • SLE sera were screened for the presence of surface neuronal antibodies using a mixed hemadsorption assay [24].
  • The hemadsorption and neuraminidase activities of the chimeric viruses showed significant differences from those of their parental strains, but heterotypic F and HN pairs were equally effective in fusion promotion [25].
  • Otherwise, the presence of the heterologous transmembrane and cytoplasmic domains had little effect on the processing of the HN or G ectodomain, as assayed by the acquisition of N-linked and O-linked carbohydrates, transport to the cell surface and, in the case of HN, folding, oligomerization, and hemadsorption activity [26].

Analytical, diagnostic and therapeutic context of Hemadsorption

  • Paired sera from 763 children and adults admitted to the hospital for acute respiratory disease were tested for significant antibody titer rises in the hemagglutination inhibition (HI) test and for specific IgM antibody with the hemadsorption immunosorbent techniques (HIT) [27].
  • The increase in hemadsorption is accompanied by an alteration in distribution of receptors within the cell population as measured by flow microfluorometry using fluorescin conjugated concanavalin A, and by a decrease in the total number of receptors as measured by binding of radiolabelled concanavalin A [28].
  • Site-directed mutagenesis of the H genes were performed to confirm that the substitution of Ser --> Gly at position 546 and Asn --> Tyr at position 481 in the H protein were responsible for hemadsorption alteration [29].


  1. Identification of two amino acids in the hemagglutinin glycoprotein of measles virus (MV) that govern hemadsorption, HeLa cell fusion, and CD46 downregulation: phenotypic markers that differentiate vaccine and wild-type MV strains. Lecouturier, V., Fayolle, J., Caballero, M., Carabaña, J., Celma, M.L., Fernandez-Muñoz, R., Wild, T.F., Buckland, R. J. Virol. (1996) [Pubmed]
  2. Interaction of mouse hepatitis virus (MHV) spike glycoprotein with receptor glycoprotein MHVR is required for infection with an MHV strain that expresses the hemagglutinin-esterase glycoprotein. Gagneten, S., Gout, O., Dubois-Dalcq, M., Rottier, P., Rossen, J., Holmes, K.V. J. Virol. (1995) [Pubmed]
  3. The Vsa proteins modulate susceptibility of Mycoplasma pulmonis to complement killing, hemadsorption, and adherence to polystyrene. Simmons, W.L., Dybvig, K. Infect. Immun. (2003) [Pubmed]
  4. Meningitis due to parainfluenza virus type 3: report of two cases and review. Arisoy, E.S., Demmler, G.J., Thakar, S., Doerr, C. Clin. Infect. Dis. (1993) [Pubmed]
  5. An African swine fever virus gene with similarity to the T-lymphocyte surface antigen CD2 mediates hemadsorption. Borca, M.V., Kutish, G.F., Afonso, C.L., Irusta, P., Carrillo, C., Brun, A., Sussman, M., Rock, D.L. Virology (1994) [Pubmed]
  6. Polar appearance and nonligand induced spreading of measles virus hemagglutinin at the surface of chronically infected cells. Ehrnst, A., Sundqvist, K.G. Cell (1975) [Pubmed]
  7. Cell surface antigens of human renal cancer defined by autologous typing. Ueda, R., Shiku, H., Pfreundschuh, M., Takahashi, T., Li, L.T., Whitmore, W.F., Oettgen, H.F., Old, L.J. J. Exp. Med. (1979) [Pubmed]
  8. Enhanced expression of melanoma-associated antigens and beta 2-microglobulin on cultured human melanoma cells by interferon. Liao, S.K., Kwong, P.C., Khosravi, M., Dent, P.B. J. Natl. Cancer Inst. (1982) [Pubmed]
  9. Concanavalin A-induced agglutinability of normal, preneoplastic, and neoplastic mouse mammary cells. Asch, B.B., Medina, D. J. Natl. Cancer Inst. (1978) [Pubmed]
  10. Detection of antibody and complement complexed in vivo on membranes of human cancer cells by mixed hemadsorption techniques. Irie, K., Irie, R.F., Morton, D.L. Cancer Res. (1975) [Pubmed]
  11. An orthopoxvirus serpinlike gene controls the ability of infected cells to fuse. Turner, P.C., Moyer, R.W. J. Virol. (1992) [Pubmed]
  12. Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody. van der Logt, J.T., Heessen, F.W., van Loon, A.M., van der Veen, J. J. Clin. Microbiol. (1982) [Pubmed]
  13. Neuraminidase is essential for fowl plague virus hemagglutinin to show hemagglutinating activity. Ohuchi, M., Feldmann, A., Ohuchi, R., Klenk, H.D. Virology (1995) [Pubmed]
  14. Biological and immunogenic properties of rabies virus glycoprotein expressed by canine herpesvirus vector. Xuan, X., Tuchiya, K., Sato, I., Nishikawa, Y., Onoderaz, Y., Takashima, Y., Yamamoto, A., Katsumata, A., Iwata, A., Ueda, S., Mikami, T., Otsuka, H. Vaccine (1998) [Pubmed]
  15. Human parainfluenza virus type 3 HN-receptor interaction: effect of 4-guanidino-Neu5Ac2en on a neuraminidase-deficient variant. Porotto, M., Greengard, O., Poltoratskaia, N., Horga, M.A., Moscona, A. J. Virol. (2001) [Pubmed]
  16. Variable infection of Vero cells and homologous interference after co-cultivation with HeLa cells with persistent defective infection by Edmonston measles virus. Rustigian, R., Winston, S.H., Darlington, R.W. Infect. Immun. (1979) [Pubmed]
  17. Radioimmunodetection of a transplantable human bladder carcinoma in a nude mouse. Gross, M.D., Skinner, R.W., Grossman, H.B. Investigative radiology. (1984) [Pubmed]
  18. The anti-influenza virus agent 4-GU-DANA (zanamivir) inhibits cell fusion mediated by human parainfluenza virus and influenza virus HA. Greengard, O., Poltoratskaia, N., Leikina, E., Zimmerberg, J., Moscona, A. J. Virol. (2000) [Pubmed]
  19. Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line. Menna, J.H., Collins, A.R., Flanagan, T.D. Infect. Immun. (1975) [Pubmed]
  20. Hemadsorption and enzyme-linked immunosorbent assay nitrocellulose replica methods for identification of colonization factor antigen (CFA)-positive Escherichia coli colonies and for isolation of CFA-negative mutants. López-Vidal, Y., Svennerholm, A.M. J. Clin. Microbiol. (1986) [Pubmed]
  21. Conformational changes of Newcastle disease virus envelope glycoproteins triggered by gangliosides. Ferreira, L., Villar, E., Muñoz-Barroso, I. Eur. J. Biochem. (2004) [Pubmed]
  22. Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases. Cattaneo, R., Schmid, A., Spielhofer, P., Kaelin, K., Baczko, K., ter Meulen, V., Pardowitz, J., Flanagan, S., Rima, B.K., Udem, S.A. Virology (1989) [Pubmed]
  23. Comparison of approaches for augmenting the serologic response to the individually specific methylcholanthrene-induced sarcoma-Meth A: pretreatment with cyclophosphamide is most effective. Livingston, P.O., DeLeo, A.B., Jones, M., Oettgen, H.F. J. Immunol. (1983) [Pubmed]
  24. A novel neuronal antigen identified by sera from patients with systemic lupus erythematosus. Hanly, J.G., Rajaraman, S., Behmann, S., Denburg, J.A. Arthritis Rheum. (1988) [Pubmed]
  25. The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence. Huang, Z., Panda, A., Elankumaran, S., Govindarajan, D., Rockemann, D.D., Samal, S.K. J. Virol. (2004) [Pubmed]
  26. O glycosylation of glycoprotein G of human respiratory syncytial virus is specified within the divergent ectodomain. Collins, P.L. J. Virol. (1990) [Pubmed]
  27. Diagnosis of parainfluenza virus infection in children and older patients by detection of specific IgM antibody. van der Logt, J.T., van Loon, A.M., Heessen, F.W., van der Veen, J. J. Med. Virol. (1985) [Pubmed]
  28. Alterations in cell surface properties induced by modified purines. Kerr, S.J. Tumour Biol. (1985) [Pubmed]
  29. A novel amino acid position in hemagglutinin glycoprotein of measles virus is responsible for hemadsorption and CD46 binding. Li, L., Qi, Y. Arch. Virol. (2002) [Pubmed]
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