The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Electrophoresis, Polyacrylamide Gel

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Electrophoresis, Polyacrylamide Gel


Psychiatry related information on Electrophoresis, Polyacrylamide Gel

  • Recombinant tau phosphorylated by GSK-3 aligned on SDS-PAGE with the abnormally phosphorylated tau (PHF-tau) associated with the paired helical filaments in Alzheimer's disease brain [6].

High impact information on Electrophoresis, Polyacrylamide Gel

  • On SDS-PAGE, MCP migrates as two broad forms with Mrs of 59,000-68,000 and 51,000-58,000 [7].
  • Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphorylated protein that migrates at 62 kDa by SDS-PAGE and associates with the p120 ras GTPase-activating protein (GAP) [8].
  • The cross-linked cells were solubilized in Nonidet-P40, immunoprecipitated with anti-Ti (monoclonal antibody T40/25) or anti-T3 (monoclonal antibodies UCHT-1 or OKT3), and analyzed by SDS-PAGE [9].
  • The two-dimensional SDS-PAGE analysis (nonreducing followed by reducing conditions) of the DSS and BSOCOES cross-linked products revealed the specifically cross-linked bands to have Mr 40,000 and Mr 28,000 [9].
  • To initiate a genetic study of the hormonal regulation of genes coding for the three vitellogenins or yolk polypeptide precursors (YPs) in Drosophila, we have isolated from wild flies genetic variants which alter the mobility in SDS-PAGE of each YP independently [10].

Chemical compound and disease context of Electrophoresis, Polyacrylamide Gel


Biological context of Electrophoresis, Polyacrylamide Gel


Anatomical context of Electrophoresis, Polyacrylamide Gel


Associations of Electrophoresis, Polyacrylamide Gel with chemical compounds

  • Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions are consistent with the Ly-3.1 antigen containing a glycoprotein subunit with an apparent mol wt of 35,000 daltons [26].
  • Proteins in extracts of normal human epidermis were separated by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE), then transferred to nitrocellulose or 2-aminophenylthioether paper for immunoperoxidase staining [27].
  • The pattern obtained by subjecting the LPS to SDS-PAGE and staining the gel with silver nitrate suggests that L. pneumophila LPS might be atypical [28].
  • Some of the deposited C3 were complexed with membrane proteins, since 140- and 250-kD bands became significantly accumulated on SDS-PAGE by treatment with the antibodies [29].
  • In accordance with the hypothesis that C4b-associated C4b might also serve as a high-affinity C5-binding site, a small amount of C4b-C4b dimer was detected on EAC14 cells by SDS-PAGE analysis [30].

Gene context of Electrophoresis, Polyacrylamide Gel

  • The purified MCAF showed microheterogeneity yielding two bands on SDS-PAGE analysis [31].
  • It was found that the CD59 antigen is a small protein (approximately 20 kD as judged by SDS-PAGE, 11.5 kD predicted from the isolated cDNA) sometimes associated with larger components (45 and 80 kD) in urine [32].
  • Immunoblot analysis using a polyclonal antiserum against the cytoplasmic domain showed that beta pat-3 migrates in SDS-PAGE with apparent M(r) of 109 k and 120 k under nonreducing and reducing conditions, respectively [33].
  • Anillin migrates at 190 kD on SDS-PAGE [34].
  • NOP3-specific antibodies recognize a 60-kD protein by SDS-PAGE and decorate the nucleolus and the surrounding nucleoplasm [35].

Analytical, diagnostic and therapeutic context of Electrophoresis, Polyacrylamide Gel


  1. Virus envelope protein of HTLV-III represents major target antigen for antibodies in AIDS patients. Barin, F., McLane, M.F., Allan, J.S., Lee, T.H., Groopman, J.E., Essex, M. Science (1985) [Pubmed]
  2. Effects of pyridoxine on serum protein expression in hepatoma-bearing rats. Tryfiates, G.P. J. Natl. Cancer Inst. (1981) [Pubmed]
  3. Filamentous protein of basal cell epithelioma: characteristics in vivo and in vitro. Kubilus, J., Baden, H.P., McGilvray, N. J. Natl. Cancer Inst. (1980) [Pubmed]
  4. A circulating ligand for galectin-3 is a haptoglobin-related glycoprotein elevated in individuals with colon cancer. Bresalier, R.S., Byrd, J.C., Tessler, D., Lebel, J., Koomen, J., Hawke, D., Half, E., Liu, K.F., Mazurek, N. Gastroenterology (2004) [Pubmed]
  5. A novel sterol 14alpha-demethylase/ferredoxin fusion protein (MCCYP51FX) from Methylococcus capsulatus represents a new class of the cytochrome P450 superfamily. Jackson, C.J., Lamb, D.C., Marczylo, T.H., Warrilow, A.G., Manning, N.J., Lowe, D.J., Kelly, D.E., Kelly, S.L. J. Biol. Chem. (2002) [Pubmed]
  6. Glycogen synthase kinase-3 induces Alzheimer's disease-like phosphorylation of tau: generation of paired helical filament epitopes and neuronal localisation of the kinase. Hanger, D.P., Hughes, K., Woodgett, J.R., Brion, J.P., Anderton, B.H. Neurosci. Lett. (1992) [Pubmed]
  7. Membrane cofactor protein (MCP or CD46): newest member of the regulators of complement activation gene cluster. Liszewski, M.K., Post, T.W., Atkinson, J.P. Annu. Rev. Immunol. (1991) [Pubmed]
  8. p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Carpino, N., Wisniewski, D., Strife, A., Marshak, D., Kobayashi, R., Stillman, B., Clarkson, B. Cell (1997) [Pubmed]
  9. Cross-linking of human T cell receptor proteins: association between the T cell idiotype beta subunit and the T3 glycoprotein heavy subunit. Brenner, M.B., Trowbridge, I.S., Strominger, J.L. Cell (1985) [Pubmed]
  10. Genetic analysis of the hormonally regulated yolk polypeptide genes in D. melanogaster. Postlethwait, J.H., Jowett, T. Cell (1980) [Pubmed]
  11. Identification of NdhL and Ssl1690 (NdhO) in NDH-1L and NDH-1M complexes of Synechocystis sp. PCC 6803. Battchikova, N., Zhang, P., Rudd, S., Ogawa, T., Aro, E.M. J. Biol. Chem. (2005) [Pubmed]
  12. Molecular cloning and expression in Escherichia coli K-12 of the O101 rfb region from E. coli B41 (O101:K99/F41) and the genetic relationship to other O101 rfb loci. Heuzenroeder, M.W., Beger, D.W., Thomas, C.J., Manning, P.A. Mol. Microbiol. (1989) [Pubmed]
  13. Two precursors of the heat-stable enterotoxin of Escherichia coli: evidence of extracellular processing. Rasheed, J.K., Guzmán-Verduzco, L.M., Kupersztoch, Y.M. Mol. Microbiol. (1990) [Pubmed]
  14. Demonstration of a sensory rhodopsin in eubacteria. Jung, K.H., Trivedi, V.D., Spudich, J.L. Mol. Microbiol. (2003) [Pubmed]
  15. Production of antibody to individual polypeptides derived from purified hepatitis B surface antigen. Dreesman, G.R., Chairez, R., Suarez, M., Hollinger, F.B., Courtney, R.J., Melnick, J.L. J. Virol. (1975) [Pubmed]
  16. Vascular permeability factor: a tumor-derived polypeptide that induces endothelial cell and monocyte procoagulant activity, and promotes monocyte migration. Clauss, M., Gerlach, M., Gerlach, H., Brett, J., Wang, F., Familletti, P.C., Pan, Y.C., Olander, J.V., Connolly, D.T., Stern, D. J. Exp. Med. (1990) [Pubmed]
  17. Cloning and characterization of the Salmonella typhimurium-specific chemoreceptor Tcp for taxis to citrate and from phenol. Yamamoto, K., Imae, Y. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  18. Biochemical characterization of the neutrophil-specific antigen NB1. Stroncek, D.F., Skubitz, K.M., McCullough, J.J. Blood (1990) [Pubmed]
  19. Synapsin I in PC12 cells. II. Evidence for regulation by NGF of phosphorylation at a novel site. Romano, C., Nichols, R.A., Greengard, P. J. Neurosci. (1987) [Pubmed]
  20. ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions. II. Molecular cloning and nucleotide sequence. Kurihara, T., Ehrlich, M.E., Horiuchi, J., Nasu, T., Greengard, P. J. Neurosci. (1989) [Pubmed]
  21. Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis. Shackelford, D.A., Strominger, J.L. J. Exp. Med. (1980) [Pubmed]
  22. Phosphorylation of chlamydomonas reinhardi chloroplast membrane proteins in vivo and in vitro. Owens, G.C., Ohad, I. J. Cell Biol. (1982) [Pubmed]
  23. The membrane glycoprotein Ia-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen. Staatz, W.D., Rajpara, S.M., Wayner, E.A., Carter, W.G., Santoro, S.A. J. Cell Biol. (1989) [Pubmed]
  24. The mammalian 43-kD acetylcholine receptor-associated protein (RAPsyn) is expressed in some nonmuscle cells. Musil, L.S., Frail, D.E., Merlie, J.P. J. Cell Biol. (1989) [Pubmed]
  25. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. Dustin, M.L., Springer, T.A. J. Cell Biol. (1988) [Pubmed]
  26. The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization. Durda, P.J., Gottlieb, P.D. J. Exp. Med. (1976) [Pubmed]
  27. Human autoantibodies against a desmosomal core protein in pemphigus foliaceus. Koulu, L., Kusumi, A., Steinberg, M.S., Klaus-Kovtun, V., Stanley, J.R. J. Exp. Med. (1984) [Pubmed]
  28. Isolation and characterization of the cytoplasmic and outer membranes of the Legionnaires' disease bacterium (Legionella pneumophila). Gabay, J.E., Horwitz, M.A. J. Exp. Med. (1985) [Pubmed]
  29. Complement-mediated tumor cell damage induced by antibodies against membrane cofactor protein (MCP, CD46). Seya, T., Hara, T., Matsumoto, M., Sugita, Y., Akedo, H. J. Exp. Med. (1990) [Pubmed]
  30. Covalent association of C3b with C4b within C5 convertase of the classical complement pathway. Takata, Y., Kinoshita, T., Kozono, H., Takeda, J., Tanaka, E., Hong, K., Inoue, K. J. Exp. Med. (1987) [Pubmed]
  31. Properties of monocyte chemotactic and activating factor (MCAF) purified from a human fibrosarcoma cell line. Zachariae, C.O., Anderson, A.O., Thompson, H.L., Appella, E., Mantovani, A., Oppenheim, J.J., Matsushima, K. J. Exp. Med. (1990) [Pubmed]
  32. CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. Davies, A., Simmons, D.L., Hale, G., Harrison, R.A., Tighe, H., Lachmann, P.J., Waldmann, H. J. Exp. Med. (1989) [Pubmed]
  33. Characterization of beta pat-3 heterodimers, a family of essential integrin receptors in C. elegans. Gettner, S.N., Kenyon, C., Reichardt, L.F. J. Cell Biol. (1995) [Pubmed]
  34. Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex. Field, C.M., Alberts, B.M. J. Cell Biol. (1995) [Pubmed]
  35. NOP3 is an essential yeast protein which is required for pre-rRNA processing. Russell, I.D., Tollervey, D. J. Cell Biol. (1992) [Pubmed]
  36. Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. Shirai, T., Yamaguchi, H., Ito, H., Todd, C.W., Wallace, R.B. Nature (1985) [Pubmed]
  37. Soluble cytokine receptors are present in normal human urine. Novick, D., Engelmann, H., Wallach, D., Rubinstein, M. J. Exp. Med. (1989) [Pubmed]
  38. Identification of membrane-bound CR1 (CD35) in human urine: evidence for its release by glomerular podocytes. Pascual, M., Steiger, G., Sadallah, S., Paccaud, J.P., Carpentier, J.L., James, R., Schifferli, J.A. J. Exp. Med. (1994) [Pubmed]
  39. Purification and partial biochemical characterization of normal human interleukin 1. Schmidt, J.A. J. Exp. Med. (1984) [Pubmed]
  40. Sub-minimal inhibitory concentrations of cefmetazole enhance serum bactericidal activity in vitro by amplifying poly-C9 deposition. Schweinle, J.E., Nishiyasu, M. J. Clin. Invest. (1992) [Pubmed]
WikiGenes - Universities