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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Mutagenesis, Site-Directed

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Disease relevance of Mutagenesis, Site-Directed


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High impact information on Mutagenesis, Site-Directed

  • That these contacts are involved with DNA binding is supported by recurrent features of a newly compiled set of homology groups of 13 variable regions of heavy chains (VH) and 11 variable regions of light chains (VL), characteristic pattern of somatic mutations, and the results of site-directed mutagenesis [7].
  • Site-directed mutagenesis studies indicate the involvement of specific amino acid residues in the proposed transmembrane segment 6 for disulfonic stilbene blockade and segments 6 and 12 for arylaminobenzoate blockade [8].
  • E75 absorption spectra, resistance to denaturants, and effects of site-directed mutagenesis indicate a single, coordinately bound heme molecule [9].
  • We describe the crystal structure of Neurospora DIM-5, a histone H3 lysine 9 methyltranferase (HKMT), determined at 1.98 A resolution, as well as results of biochemical characterization and site-directed mutagenesis of key residues [10].
  • Residues in the Hh-C17 active site have been identified, and their role in Hedgehog autoprocessing probed by site-directed mutagenesis [11].

Chemical compound and disease context of Mutagenesis, Site-Directed


Biological context of Mutagenesis, Site-Directed


Anatomical context of Mutagenesis, Site-Directed

  • By oligonucleotide site-directed mutagenesis we have engineered a single amino acid change in a mouse IgG2b antibody (Glu 235----Leu) which now enables the antibody to bind to the FcRI (high affinity) receptor on human monocytes with a 100-fold improvement in affinity [22].
  • Using a Ti beta chain-deficient mutant of the cell line Jurkat, site-directed mutagenesis and transfection of Ti beta chain cDNA was performed to assess the importance of the lysine residue at position 290 within the membrane-spanning domain of the Ti beta chain to expression of the TCR complex [23].
  • Here, we show that two neighboring histidine residues on NR2A represent the critical determinant (termed the "short spacer") for high-affinity, voltage-independent Zn2+ inhibition using the Xenopus oocyte expression system and site-directed mutagenesis [24].
  • Since HuGH, but not GH of other species, effectively binds to the HuPRL receptor (HuPRL-R), we used a group of HuGH variants created by site-directed mutagenesis to identify the receptor on human neutrophils responsible for HuGH priming [25].
  • The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes [26].

Associations of Mutagenesis, Site-Directed with chemical compounds


Gene context of Mutagenesis, Site-Directed


Analytical, diagnostic and therapeutic context of Mutagenesis, Site-Directed


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  14. Amantadine selection of a mutant influenza virus containing an acid-stable hemagglutinin glycoprotein: evidence for virus-specific regulation of the pH of glycoprotein transport vesicles. Steinhauer, D.A., Wharton, S.A., Skehel, J.J., Wiley, D.C., Hay, A.J. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
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  16. Site-directed mutagenesis identifies a tyrosine radical involved in the photosynthetic oxygen-evolving system. Debus, R.J., Barry, B.A., Babcock, G.T., McIntosh, L. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  17. Mutagenesis of Fujinami sarcoma virus: evidence that tyrosine phosphorylation of P130gag-fps modulates its biological activity. Weinmaster, G., Zoller, M.J., Smith, M., Hinze, E., Pawson, T. Cell (1984) [Pubmed]
  18. Idealization of the hydrophobic segment of the alkaline phosphatase signal peptide. Kendall, D.A., Bock, S.C., Kaiser, E.T. Nature (1986) [Pubmed]
  19. Casein kinase II inhibits the DNA-binding activity of Max homodimers but not Myc/Max heterodimers. Berberich, S.J., Cole, M.D. Genes Dev. (1992) [Pubmed]
  20. Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequences. Grandien, K., Sommer, R.J. Genes Dev. (2001) [Pubmed]
  21. The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis. Tanner, N.K., Cordin, O., Banroques, J., Doère, M., Linder, P. Mol. Cell (2003) [Pubmed]
  22. Localization of the binding site for the human high-affinity Fc receptor on IgG. Duncan, A.R., Woof, J.M., Partridge, L.J., Burton, D.R., Winter, G. Nature (1988) [Pubmed]
  23. The lysine residue in the membrane-spanning domain of the beta chain is necessary for cell surface expression of the T cell antigen receptor. Morley, B.J., Chin, K.N., Newton, M.E., Weiss, A. J. Exp. Med. (1988) [Pubmed]
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