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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

DNA microarrays and beyond: completing the journey from tissue to cell.

For the cell biologist, identifying changes in gene expression using DNA microarrays is just the start of a long journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.[1]

References

  1. DNA microarrays and beyond: completing the journey from tissue to cell. Mills, J.C., Roth, K.A., Cagan, R.L., Gordon, J.I. Nat. Cell Biol. (2001) [Pubmed]
 
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