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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Human neutrophils isolated from peripheral blood contain Ku protein but not DNA-dependent protein kinase.

Ku protein, a heterodimer of 70kDa (Ku70) and 86kDa ( Ku86) polypeptides, is involved in non-homologous DNA end-joining (NHEJ) of DNA double-strand break repair and V(D)J recombination in combination with the catalytic component of DNA-dependent protein kinase (p470). Although Ku protein is known to be ubiquitously present in eukaryotic cells, it was previously reported to be absent in mature neutrophils. Using a mixture of protease inhibitors in the isolation procedure of neutrophils from human peripheral blood, we were able to detect Ku in the neutrophils by immunoblot and flow-cytometric analyses. Transcripts of Ku70 and Ku86 genes were also detected by the reverse transcriptase-polymerase chain reaction (RT-PCR), and Ku protein was shown to be localized in the nucleus of neutrophils as a heterodimer. Like poly(ADP-ribose) polymerase-1, neither mRNA nor protein of p470 was detected in the neutrophils. These results suggest that Ku is involved independently of p470 in DNA metabolism and signal transduction.[1]

References

  1. Human neutrophils isolated from peripheral blood contain Ku protein but not DNA-dependent protein kinase. Kurosawa, A., Shinohara, K., Watanabe, F., Shimizu-Saito, K., Koiwai, O., Yamamoto, K., Teraoka, H. Int. J. Biochem. Cell Biol. (2003) [Pubmed]
 
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