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Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
Single microtubules from squid axoplasm support bidirectional movement of organelles. We previously purified a microtubule translocator (kinesin) that moves latex beads in only one direction along microtubules. In this study, a polar array of microtubules assembled off of centrosomes in vitro was used to demonstrate that kinesin moves latex beads from the minus to the plus ends of microtubules, a direction that corresponds to anterograde transport in the axon. A crude solubilized fraction from squid axoplasm (S1a), however, generates bidirectional movement of beads along microtubules. Retrograde bead movement (1.4 micron/sec) is inhibited by N-ethylmaleimide and 20 microM vanadate while anterograde movement (0.6 micron/sec) is unaffected by these agents. Furthermore, a monoclonal antibody against kinesin, when coupled to Sepharose, removes the anterograde, but not the retrograde, bead translocator from S1a. These results indicate that there is a retrograde bead translocator which is pharmacologically and immunologically distinct from kinesin.[1]References
- Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro. Vale, R.D., Schnapp, B.J., Mitchison, T., Steuer, E., Reese, T.S., Sheetz, M.P. Cell (1985) [Pubmed]
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