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Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients.

Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis ( MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequence in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.[1]

References

  1. Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients. Reddy, E.P., Sandberg-Wollheim, M., Mettus, R.V., Ray, P.E., DeFreitas, E., Koprowski, H. Science (1989) [Pubmed]
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