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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein.

The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.[1]

References

  1. Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein. Oosterom-Dragon, E.A., Ginsberg, H.S. J. Virol. (1980) [Pubmed]
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