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SIN3 works through two different promoter elements to regulate INO1 gene expression in yeast.

The SIN3 global regulatory factor affects expression of many yeast genes, including the phospholipid biosynthetic gene, INO1. Mutations in the SIN3 gene result in elevated levels of INO1 expression under conditions that normally confer full repression of INO1 transcription, indicating that SIN3 is a negative regulator of INO1. In this study, the INO1 promoter was analyzed for sequences that play a role in responding to SIN3-mediated repression. Two distinct promoter elements, the upstream repression sequence (URS1) and the INO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression.[1]

References

  1. SIN3 works through two different promoter elements to regulate INO1 gene expression in yeast. Slekar, K.H., Henry, S.A. Nucleic Acids Res. (1995) [Pubmed]
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