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Micellar electrokinetic capillary chromatography for determination of indolyl glucosinolates and transformation products thereof.
Micellar electrokinetic capillary chromatography based on dodecyltrimethylammonium bromide as a surfactant has been developed for the qualitative and quantitative determination of indolylic compounds. Indolyl glucosinolates (indol-3-ylmethylglucosinolates), its degradation products, and further transformation products thereof have been included in the studies. The influence of various parameters on the separation has been investigated, resulting in efficient separation, even for structurally closely related compounds, with theoretical plate number per meter of capillary of up to 370,000 for some compounds. Repeatability and linearity of the method have been determined giving relative standard deviations for the migration times of the compounds between 0.5 and 0.9% and linear correlation coefficients between 0.9924 and 0.9996, respectively. The method can be successfully applied to follow the degradation of indolyl glucosinolates in detail.[1]References
- Micellar electrokinetic capillary chromatography for determination of indolyl glucosinolates and transformation products thereof. Feldl, C., Møller, P., Otte, J., Sørensen, H. Anal. Biochem. (1994) [Pubmed]
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