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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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DeLange, F. et al.

Tyrosine structural changes detected during the photoactivation of rhodopsin.

We present the first Fourier transform infrared (FTIR) analysis of an isotope-labeled eukaryotic membrane protein. A combination of isotope labeling and FTIR difference spectroscopy was used to investigate the possible involvement of tyrosines in the photoactivation of rhodopsin ( Rho). Rho --> MII difference spectra were obtained at 10 degrees C for unlabeled recombinant Rho and isotope-labeled L-[ring-2H4]Tyr- Rho expressed in Spodoptera frugiperda cells grown on a stringent culture medium containing enriched L-[ring-2H4]Tyr and isolated using a His6 tag. A comparison of these difference spectra revealed reproducible changes in bands that correspond to tyrosine and tyrosinate vibrational modes. A similar pattern of tyrosine/tyrosinate bands has also been observed in the bR --> M transition in bacteriorhodopsin, although the sign of the bands is reversed. In bacteriorhodopsin, these bands were assigned to Tyr-185, which along with Pro-186 in the F-helix, may form a hinge that facilitates alpha-helix movement.[1]

References

Tyrosine structural changes detected during the photoactivation of rhodopsin. DeLange, F., Klaassen, C.H., Wallace-Williams, S.E., Bovee-Geurts, P.H., Liu, X.M., DeGrip, W.J., Rothschild, K.J. J. Biol. Chem. (1998) [Pubmed]

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