Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

AGN-PC-000XS0 2-[3,4-dihydroxy-2,5- bis(hydroxymethyl)oxo...

Synonyms: CTK1G9656, AR-1J1608, AC1L2SS7, AC1Q70KA, 562-68-5, ...

Van Riet, L. et al., Zuther, E. et al., Kawakami, A. et al.

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Disease relevance of 562-68-5

Potato ( Solanum tuberosum L.) plants expressing the invertase under the control of the Agrobacterium tumefaciens rolC promoter produced the trisaccharide 6-kestose in leaves, which was transported via the phloem and accumulated in tubers of transgenic plants [1].

High impact information on 562-68-5

Functional characterization of the recombinant proteins in Pichia pastoris demonstrated that the recombinant enzyme had FEH activity towards the pure compounds 1-kestose, 6-kestose, 1,1-nystose and 1,1,1-kestopentaose [2].
Functionality tests of the cDNA performed after heterologous expression in the yeast Pichia pastoris showed--as did the native enzyme from wheat--a very high activity of the produced protein against bacterial levan, 6-kestose, and phlein whilst sucrose and inulin were not used as substrates [3].

References

Expression of a yeast-derived invertase in companion cells results in long-distance transport of a trisaccharide in an apoplastic loader and influences sucrose transport. Zuther, E., Kwart, M., Willmitzer, L., Heyer, A.G. Planta (2004) [Pubmed]
Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose. Kawakami, A., Yoshida, M., Van den Ende, W. Gene (2005) [Pubmed]
Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.). Van Riet, L., Nagaraj, V., Van den Ende, W., Clerens, S., Wiemken, A., Van Laere, A. J. Exp. Bot. (2006) [Pubmed]

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