High impact information on methanoic acid

• Chromatographic separation was achieved on a HyPurity Advance column with a mobile phase consisting of ammonium formate buffer (10 mM, pH 8.0) and acetonitrile (70:30, v/v) [1].
• Satisfactory separation of the 12 cytokinin standards was achieved using a 25 mM ammonium formate/formic acid buffer (pH 3.4) and 3% ACN v/v with a separation voltage of 25 kV [2].
• Glyphosate separation was achieved by using a bare fused-silica capillary column, operated in the reversed-polarity mode, using ammonium formate buffer, pH 2 [3].
• For the CE separations ammonium formate buffer solutions (70 or 100 mM, pH 3.0 or 4.0) containing 10% methanol or 20-60% acetonitrile as additives were used [4].
• These acylamides underwent rapid cyclization to 2,4-disubstituted quinazolines (and benzoquinazolines) in the presence of ammonium formate under microwave activation [5].

Biological context of methanoic acid

• Dehalogenation of perophoramidine (1) by ammonium formate catalyzed transfer hydrogenation confirmed the type and number of halogen atoms present in 1 [6].
• The mean cationic concentrations for trace elements in the 22 bacterial cultures grown in the basal seawater medium to constant optical density and washed three times with 0.5 M ammonium formate were: Mn, 2.4 +/- 3.8; Fe, 262 +/- 112; Ni, 2.3 +/- 1.8; Cu, 24 +/- 17; Zn, 146 +/- 72; Cd, 3.8 +/- 2.5; and Pb, 17 +/- 21 ppm (dry weight) [7].
• Power saturation studies indicate that the energy transfer between the spins and the lattice is fast in ammonium formate, which gives the possibility of using high microwave power without saturation to further increase the sensitivity [8].
• Reduction of this mixture by transfer hydrogenation using ammonium formate in methanol and Pd/C as catalyst afforded 1,4-dideoxy-1,4-imino-D-glucitol (4), the structure of which was proved after acetylation by 1H-n.m.r. spectroscopy [9].
• Signal suppression tests showed that electrospray ionization (ESI) is a more effective ionization method than atmospheric pressure chemical ionization (APCI) for selenium species in a matrix containing pentafluoropropionic acid, heptafluorobutyric acid or ammonium formate [10].

Associations of methanoic acid with other chemical compounds

• A mixture of 25 mM ammonium formate at pH 3.0 and acetonitrile (30:70 v/v) was used as the mobile phase [11].
• Normal nucleotides were eluted with 5% methanol, 95% 1 M ammonium formate, pH 3 [12].
• Solvent pH and the ratio of aqueous ammonium formate/acetonitrile had minor effects on the degree of fragmentation of haloperidol in a wide range [13].
• During direct liquid introduction (DLI) liquid chromatography-mass spectrometry (LC-MS), the detectability of acetone is shown to be predictable, based on consideration of the gas-phase acidities and basicities of solvents methanol and acetonitrile and modifiers formic acid and ammonium formate [14].
• Binding of purine nucleotides to ammonium formate during gel filtration on Sephadex G-10 [15].

Gene context of methanoic acid

• Capillary zone electrophoresis (CZE) of five model proteins (lysozyme, myoglobin, ribonuclease A, alpha-lactalbumin, and trypsinogen), using ammonium formate as the electrophoretic buffer and triethylamine (TEA) as a buffer additive at pH 2.5, was used for protein separation [16].
• Chromatographic separation involved an X-TERRA MS C18, 3.5 microm (100 mm x 1 mm i.d.) column together with a 25-min long gradient of acetonitrile in pH 3, 2 mM ammonium formate delivered at a 50 microl/min flow rate [17].
• By Dowex AG1 x 8 chromatography the polar head group could be separated into three radioactive peaks eluting at 100 mM (peak I), 200 mM (peak II) and 500 mM (peak III) ammonium formate, respectively [18].
• L-amino acids were produced with thermostable L-amino acid dehydrogenase and formate dehydrogenase (FDH) from alpha-keto acids and ammonium formate with only an intracellular pool of NAD+ for the regeneration of NADH [19].
• The fatty acid p-bromophenacyl esters were analysed on a 10 RP-18 column with methanol-acetonitrile-0.01 M ammonium formate as mobile phase and also on a 10 RP-8 column with acetonitrile-0.01 M ammonium formate as mobile phase [20].

Analytical, diagnostic and therapeutic context of methanoic acid

• Isolation of HMG-CoA utilizes ion-exchange chromatography in a gradient of ammonium formate, which is subsequently removed by lyophilization [21].
• For clean-up of the crude sample, the resuspended extract was applied to a Bond Elut PAS (primary/secondary amine cartridge), and then SAs were eluted from the cartridge using 5 ml of 20 v/v% acetonitrile-0.05 M ammonium formate before being analysed by HPLC [22].
• After enzymatic digestion of DNA, the 2'-deoxyribonucleoside 3'-monophosphates may be further separated in less than 30 min by high-performance liquid chromatography on a Hypersil octadecylsilylsilica gel column in the ion-suppression mode by using either ammonium formate (0.05 M, pH 6.5) or sodium succinate (0.02 M, pH 6.0) [23].
• The bound fraction eluted with 50 mM ammonium formate buffer and separated using size exclusion chromatography producing the pectins rhamnogaracturonan (RG)-I and RG-II with apparent molecular masses of 15000 and 11000, respectively [24].
• The impurities were separated from the main drug compound by capillary electrophoresis (CE) using an ammonium formate buffer at low pH in an untreated fused silica capillary [25].

References