High impact information on ATP beta S

• The order as a phosphate donor as determined by both methods in the phosphomevalonate kinase reaction is ATP beta S greater than ATP alpha S greater than ATP greater than ATP gamma S. Except for ATP gamma S, the equilibrium is shifted in the direction of formation of ADP alpha S and ADP beta S relative to ADP formation [1].
• About six times more MMTV RNA is initiated with GTP beta S than with ATP beta S [2].
• With Mg(II), pyridoxal kinase exhibits stereoselectivity for the A diastereomer of ATP beta S, Vmax ratio, A/B = 30 [3].
• Stereochemical and kinetic studies on the action of the catalytic subunit of bovine cardiac muscle adenosine 3',5'-monophosphate dependent protein kinase using metal ion complexes of ATP beta S [4].
• To probe the active site structure of protein kinase C stereochemical studies were carried out by using ATP beta S [5].

Biological context of ATP beta S

• Additional studies carried out with exchange inert metal-nucleotide complexes involving rhodium and the Mg2+ and Cd2+ complexes of ATP beta S and ATP alpha S imply that the rate-limiting step in the ATPase reaction pathway occurs subsequent to the P gamma-O-P beta bond cleavage steps, perhaps at the level of Mg(ADP)(Pi) hydrolysis or MgADP release [6].

Anatomical context of ATP beta S

• With ATP beta S and GTP beta S, region-specific initiation of MMTV RNA chains was detected in the cell free system [7].
• When tested against electrically-evoked twitch responses of the vas deferens the order of potencies was reversed with ATP being most active and ATP beta S least active [8].
• Finally, in contrast to soluble F1, F1 bound to F0 in the inner mitochondrial membrane failed to discriminate between the Mg2+ complexes of the ATP beta S isomers [6].

Associations of ATP beta S with other chemical compounds

• Adenosine 5'0-(2-thiotriphosphate) (ATP beta S) was more active than adenosine 5'0(1-thiotriphosphate) (ATP alpha S) and ATP in producing contractile responses on the vas deferens of rat and guinea-pig, and guinea-pig bladder, though the difference of potency was less marked for producing relaxation of the carbachol-contracted taenia coli [8].

Gene context of ATP beta S

• The kinetic parameters, Km and V, for the mammalian hexokinase reaction have been determined for ATP beta S in the presence of the three divalent metal ions [9].
• The diastereomers of adenosine 5'-O-(2-thiotriphosphate) (ATP beta S) in the presence of Mg2+, Co2+ and Cd2+ have been used to determine the stereospecificity of the metal-nucleotide binding site of rat muscle hexokinase type II and rat liver glucokinase by the method developed by Jaffe and Cohn [J. Biol. Chem. 254, 10839-10845 (1979)] [9].

References