High impact information on Gly-gln

• Renal metabolism of glycylglutamine resulted in the release of constituent amino acids that were largely utilized by the liver in the postabsorptive state and by skeletal muscle in starvation [1].
• Immunohistochemical localization of the pro-opiomelanocortin-gene product, glycylglutamine, in the intermediate pituitary [2].
• Functional expression was determined as the rate of uptake of glycylglutamine (Gly-Gln) by brush-border membrane vesicles (BBMVs) prepared from the jejunum of fed and fasted rats [3].
• Uptake and hydrolysis of glycylglutamine at the blood-brain barrier [4].
• When isolated brain capillaries were incubated with glycylglutamine, uptake was only modestly above background activity and appeared to be due to nonspecific binding [4].

Anatomical context of Gly-gln

• Finally, the plasma membrane of brain capillaries lacked hydrolase activity against glycylglutamine [4].
• The existence of an oligopeptide transporter was probed with the uptake of glycylglutamine (Gly-Gln) by membrane vesicles prepared from renal lysosomes [5].
• We used a human intestinal cell monolayer (Caco-2) as the in vitro model of human small intestine and glycylglutamine (Gly-Gln) as the model substrate for Pept-1 [6].

Analytical, diagnostic and therapeutic context of Gly-gln

• Antisera which recognize glycylglutamine were raised in rabbits and used for immunohistochemistry [2].
• The present study was designed to investigate the metabolism of glycylglutamine and its effects on organ balances of amino acids during intravenous infusion of this dipeptide (100 mumol.h-1.kg-1) in postabsorptive and briefly starved (84-86 h) human subjects [7].

References