Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

AG-H-23289 (5S,12R)-5,12-dihydroxyicosa- 6,8,10,14...

Synonyms: CBiol_001897, CTK8F3990, Bio1_000183, Bio1_000672, Bio1_001161, ...

Udén, A.M. et al., McColl, S.R. et al., Schönfeld, W. et al., von Schacky, C. et al., Schlüter, B. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on C05950

Human neutrophils stimulated with PAF in the range of 10(-5) to 10(-7) M for 15 min released small quantities of leukotriene B4 and its omega-oxidation products, 20-OH- and 20-COOH-leukotriene B4 in amounts that were detectable by enzyme immunoassay [1].
In contrast to the findings in vitro, however, levels of LTB4, 20-OH-LTB4, and 20-COOH-LTB4 were unaltered by the dietary intervention [2].
When LTB4 was incubated with the sonicate in the presence of NADPH and NAD+, 20-OH-LTB4 and 20-COOH-LTB4 appeared concomitantly with the disappearance of LTB4 [3].
During the first week after injury a significantly reduced capacity to generate LTB4 and an increased metabolism of LTB4 into omega-oxidated products (20-OH-LTB4 and 20-COOH-LTB4) were observed after stimulation of the granulocytes with Ca ionophore [4].
The incubation of the cells (1 X 10(8) with exogenously added LTB4 resulted in the formation of omega-oxidated products (20-OH and 20-COOH-LTB4) and a novel unpolar metabolite, as identified by thin layer chromatography [5].

Anatomical context of C05950

Since it has been suggested that a lipoxygenase product might be specific chemotactic factor for eosinophils, we assessed the stimulating effects of a variety of lipoxygenase products (LTB4, 20-OH-LTB4, 20-COOH-LTB4 and 5(S)12(S)-DHETE) on both neutrophil and eosinophil migration under agarose [6].

Analytical, diagnostic and therapeutic context of C05950

Levels of LTB4, 20-OH-LTB4, 20-COOH-LTB4 as well as LTB5, 20-OH-LTB5 and 20-COOH-LTB5 were measured by high-pressure liquid chromatography (HPLC) after stimulation and extraction of a platelet-free granulocyte preparation (92% neutrophils) [7].

References

Granulocyte-macrophage colony-stimulating factor increases the synthesis of leukotriene B4 by human neutrophils in response to platelet-activating factor. Enhancement of both arachidonic acid availability and 5-lipoxygenase activation. McColl, S.R., Krump, E., Naccache, P.H., Poubelle, P.E., Braquet, P., Braquet, M., Borgeat, P. J. Immunol. (1991) [Pubmed]
Catabolism of leukotriene B5 in humans. von Schacky, C., Fahrer, C., Fischer, S. J. Lipid Res. (1990) [Pubmed]
NAD+-dependent conversion of 20-OH-LTB4 to 20-COOH-LTB4 by a cell-free system of human polymorphonuclear leukocytes. Sumimoto, H., Takeshige, K., Minakami, S. Biochem. Biophys. Res. Commun. (1985) [Pubmed]
Leukotriene generation in patients with multiple injuries. Schönfeld, W., Knöller, J., Joka, T., Obertacke, U., König, W. The Journal of trauma. (1992) [Pubmed]
Generation and metabolism of leukotrienes and release of histamine from human dispersed tonsillar cells. Schlüter, B., Schönfeld, W., König, W. Scand. J. Immunol. (1988) [Pubmed]
Effects of novel lipoxygenase products on migration of eosinophils and neutrophils in vitro. Udén, A.M., Palmblad, J., Lindgren, J.A., Malmsten, C. Int. Arch. Allergy Appl. Immunol. (1983) [Pubmed]
Dietary n-3 fatty acids accelerate catabolism of leukotriene B4 in human granulocytes. von Schacky, C., Kiefl, R., Marcus, A.J., Broekman, M.J., Kaminski, W.E. Biochim. Biophys. Acta (1993) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.