Disease relevance of NSC 8183

• In this report we demonstrate that NEM and N-phenylmaleimide (NPM) are rapidly detoxified by E. coli [1].
• These compounds undergo in situ cyclodimerization of the [4 + 2]-type upon dehydration of the precursor tertiary alcohols and form endo-adducts in the Diels-Alder reaction with N-phenylmaleimide [2].

High impact information on NSC 8183

• In addition, the role of Cys(33) and Cys(301) in that process was investigated by site-directed mutagenesis combined with chemical modification of GlcAT-I by N-phenylmaleimide [3].
• In the present study, muscle fibers are reacted with N-phenylmaleimide such that ATP hydrolysis is inhibited, i.e., the cross-bridge population under relaxing conditions is distributed only between the two states of M*ATP and A*M*ATP [4].
• Behavior of N-phenylmaleimide-reacted muscle fibers in magnesium-free rigor solution [5].
• Graphical evaluation of alkylation of myosin's SH1 and SH2: the N-phenylmaleimide reaction [6].
• The inhibitory effect increased with the increase in hydrophobicity of the NEM analogs: N-methylmaleimide less than N-ethylmaleimide less than N-phenylmaleimide [7].

Biological context of NSC 8183

• 4. A cysteine important for the activity of murine CEH appears not to be in the active site as judged by N-phenylmaleimide inhibition in the presence and absence of either (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)glycidol, a competitive inhibitor, or trans-stilbene oxide, a substrate [8].

Anatomical context of NSC 8183

• The modeling was based on the x-ray diffraction patterns from the relaxed skinned rabbit psoas muscle fibers where ATP hydrolysis was inhibited by N-phenylmaleimide treatment (S. Xu, J. Gu, G. Melvin, L. C. Yu. 2002. Biophys. J. 82:2111-2122) [9].
• The nonpolar maleimides N-ethylmaleimide (NEM) and N-phenylmaleimide (NPM) inhibited IL-2 production by 85-99%, but only when added to JURKAT cells prior to the mitogen [10].
• Interactions of sodium-proton exchange mechanism in dog red blood cells with N-phenylmaleimide [11].

Associations of NSC 8183 with other chemical compounds

• In addition, the role of the cysteine residues was investigated by site-directed mutagenesis combined with chemical modifications of XT-I by N-phenylmaleimide [12].
• Xanthine oxidase (XO), purified from buttermilk was subjected to modification with N-phenylmaleimide, p-toluene-sulfonyl chloride, and 2-mercaptobenzimidazole [13].

Gene context of NSC 8183

• Both NEM and NPM suppressed IL-2 production at doses that did not affect proliferation [10].

Analytical, diagnostic and therapeutic context of NSC 8183

• We also found that crossbridges are locked in a weakly-binding state after treatment with N-phenylmaleimide (NPM) [14].
• A combination of infrared, Raman and inelastic neutron scattering (INS) spectroscopies with density functional theory (DFT) calculations is used to provide a complete assignment of the vibrational spectra of N-phenylmaleimide and N-(perdeuterophenyl)maleimide [15].

References