Disease relevance of Neoagarobiose

• The degradation of neoagarotetraose and neoagarobiose by Cytophaga flevensis was investigated [1].
• High-level expression of a neoagarobiose-producing beta-agarase gene from Agarivorans sp. JAMB-A11 in Bacillus subtilis and enzymic properties of the recombinant enzyme [2].
• Isolation of neoagarobiose and neoagarotetraose from agarose digested by Pseudomonas elongata [3].
• Beside, neoagarobiose whitened B16 murine melanoma cells, and showed low cytotoxicity [4].

High impact information on Neoagarobiose

• Neoagarotetra-ase is highly specific for oligosaccharides containing neoagarobiose units; the rate of hydrolysis is greatest with neoagarotetraose [1].
• At the opposite extreme, under very mild methanolysis conditions such as methanolic 0.01 M HCl at 100 degrees C for 1 h, agars and gelling carrageenans were well differentiated by the respective determination of agarobiose- and carrabiose-dimethyl acetal which are well-separated on octadecyl reversed phase HPLC columns with water as eluent [5].
• The structure of the galactan sulphate of P. lanosa has been established by a combination of methylation, treatment with alkali, and partial methanolysis of the alkali-treated polysaccharide to give derivatives of agarobiose [6].

Associations of Neoagarobiose with other chemical compounds

• Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl alpha-galactoside hydrolase [7].

Analytical, diagnostic and therapeutic context of Neoagarobiose

• The principal depolymerase, a beta-agarase I, hydrolysed agar to both neoagarotetrose and neoagarobiose, as identified by thin layer chromatography [8].

References