Disease relevance of Fungitetraose

• Anaerobic fermentation of nystose by a suspension of mixed dental plaque microorganisms and by S. mutans NCTC 10449 was about half as fast as with sucrose [1].
• Glycosyltransferase from Streptococcus mutans #620 did not utilize nystose for the formation of a glucan-type polysaccharide [1].

High impact information on Fungitetraose

• This tetrasaccharide was subjected to an array of in vitro tests designed for the assessment of potential sugar substitutes before animal or human studies. beta-Fructosidase from yeast cleaved nystose at about 5% of the initial rate observed with sucrose [1].
• Carbohydrases from human jejunal mucosa did not attack nystose [1].
• The digestion of Neosugar, a mixture of 1F-(1-beta-fructofuranosyl)n-1 sucrose [n = 2, 1-kestose (GF2); n = 3, nystose (GF3); n = 4, 1F-beta-fructofuranosyl nystose (GF4)] was investigated in vitro and in vivo by using the rat [2].
• The HPLC isooligosaccharide product analysis of these transferase enzymes reveal that the four forms of enzymes are distinct and produce oligosaccharides like panose, kestose and nystose or act as hydrolytic enzymes, depending on reaction conditions [3].
• 4. In the cellular adherence experiment using nystose, the amount of serotype d cells that adhered to glass surface was almost zero [4].

References