High impact information on phosphenic acid

• The method is based on the direct analysis of the metaphosphoric acid erythrocyte extract containing diethylenetriaminepentaacetic acid [1].
• Five percent metaphosphoric acid extracts of mononuclear leukocytes (MN), polymorphonuclear leukocytes (PMN), platelets, or plasma were mixed with the IA internal standard and diluted with an EDTA-cysteine solution [2].
• FSH binding inhibitor was concentrated from large volumes of testis supernatant by precipitation of inert material with metaphosphoric acid, concentration/desalting of the resulting supernatant by ultrafiltration (Amicon UM-05 membranes) and lyophilization [3].
• The reaction was terminated by the addition of metaphosphoric acid and liberated hippuric acid in the supernatant was quantitated directly by reversed-phase high-performance liquid chromatography [4].
• The assay can be applied directly to serum specimens (direct method) and also to sera with a prior deproteinization step with metaphosphoric acid [5].

Anatomical context of phosphenic acid

• Methods. The epithelium, cortex and nucleus of the lens were separated and also the whole lens was homogenized in 3% metaphosphoric acid [6].
• Chromatography of cytosol or metaphosphoric acid extract on Sephadex G-75 resulted in separation into three protein fractions designated as G-75-I, II and III in order of their elution [7].
• Gel filtration on Sephadex G-75 of the metaphosphoric acid extracts of liver, kidney, testis and ovary revealed an identical elution pattern for ovarian and testicular inhibin [7].

Associations of phosphenic acid with other chemical compounds

• Purification was achieved by homogeneizing chicken pituitaries in a protease inhibitor solution (0.5 mM PMSF and aprotinin, 50 KIU/ml); the supernatant of the alkaline extract (pH 9.5) was precipitated with 0.15 M ammonium sulfate and metaphosphoric acid, pH 4 [8].
• For hormone estimation, the tissue was homogenised/powdered and suspended in phosphate buffer (for OT and progesterone), 0.1 M trichloracetic acid (TCA; for catecholamines) or in ice-cold metaphosphoric acid (for ascorbic acid) [9].

Gene context of phosphenic acid

• APM extracted with 5% metaphosphoric acid was separated by a LiChrospher 100 RP18 column within 20 min [10].
• Prior to LC analysis, BSA solutions or rat plasma samples were treated with metaphosphoric acid to precipitate protein [11].
• Blood, liver, lungs, heart, kidneys, brain, testes and lenses were excised rapidly and were extracted with cold metaphosphoric acid [12].

Analytical, diagnostic and therapeutic context of phosphenic acid

• The plasma samples are stabilized with 5 mM metaphosphoric acid, centrifugated at 4 degrees C before HPLC analysis [13].
• Isolation of these factors has been achieved by using three different procedures: cytosol preparation, metaphosphoric acid extraction and ultrafiltration [7].

References