Disease relevance of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• The present study demonstrates that the tabun toxicity in mice is more effectively reduced when PANPAL prophylactically is administered than in case of treatment with methoxime and cholinergic drug alone [1].

High impact information on 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• The peaks obtained by the latter method were derivatized to the methoxime methyl ester trimethyl silyl ether and analyzed by gas-liquid chromatography-mass spectrometry [2].
• Alprenolone methoxime is stable in isotonic phosphate vehicle but undergoes enzymatic hydrolysis to the corresponding ketone in the eye [3].
• The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime- and ethoxime-trimethylsilyl (TMS) derivatives of C(4) to C(7) sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled (13)C analogs [4].
• The analytes were isolated from acidified human plasma by solid-phase extraction by means of Bond Elut C18 cartridges and derivatized to the pentafluorobenzyl (PFB) ester methoxime [5].
• Their methoxime and 2,4-dinitrophenylsemicarbazone derivatives were resolved on OV-17 and OV-1 stationary phases, respectively [6].

Chemical compound and disease context of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• Pretreatment with PANPAL significantly decreased tabun toxicity (nearly 4 times in methoxime and benactyzine combination and more than 4 times in atropine and methoxime mixture) [1].

Biological context of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• Based on our determinations the new K series of reactivators is far superior to pralidoxime, methoxime and BI-6, K-27 being the outstanding compound with a tg alpha value of 3.7 (nm IC50 increase per microm reactivator) which is approximately 13 times the reactivator ability of PRX [7].
• In vitro, methoxime seems to be the most efficacious reactivator of GF agent-inhibited acetylcholinesterase because the phosphorylation of acetylcholinesterase by GF agent markedly increases its affinity for the enzyme [8].

Anatomical context of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• Five oximes (K-27, K-33, K-48, BI-6 and methoxime) in comparison with pralidoxime: in vitro reactivation of red blood cell acetylcholinesterase inhibited by paraoxon [7].
• Prostaglandins PGE1 and PGE2 extracted from bovine semen, purified via silicic column chromatography were quantified by gas chromatography as their methoxime methyl ester trimethylsilyl ether derivatives [9].

Associations of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine with other chemical compounds

• The purpose of the study was to quantify in vitro the extent of oxime (pralidoxime, K-27, K-33, K-48, methoxime and BI-6) conferred protection, using paraoxon as an inhibitor [7].
• Based on our previous in vitro work the protection conferred by the various new oximes against inhibition by paraoxon as quantified by the IC(50) shift (nM increase in the IC(50) of the inhibitor per microM oxime present) is: 0.3 (PRX), 0.4 (methoxime; MMC-4), 1 (K-33), 1.2 (BI-6), 1.5 (K-48) and 3.7 (K-27) [10].
• Natrium thiosulphate did not significantly increase neither decrease the antidotal treatment efficacy in comparison with methoxime and atropine alone [1].
• After purification and high-performance liquid chromatography (HPLC) samples are derivatized to give an open-chain derivative of thromboxane B2, the methoxime pentafluorobenzyl ester tris(trimethylsilyl) ether (TXB2-MO-PFB-TMS3), most suitable for negative ion chemical ionization mass spectrometry [11].
• Steroids, mostly in the form of glucuronide and sulphate conjugates, are extracted using solid-phase cartridges, followed by enzymatic hydrolysis, re-extraction of freed steroids, formation of methoxime trimethylsilyl derivatives and analysis by sas chromatography and gas chromatography-mass spectrometry [12].

Gene context of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• AChE activity in the pontomedullar area was increased on increasing the dose of methoxime (p less than 0.005) [13].

Analytical, diagnostic and therapeutic context of 4-(nitrosomethylene)-1-[[4-(nitrosomethylene)-1-pyridyl]methyl]pyridine

• Following conversion to the methoxime, tris-trimethylsilyl, pentafluorobenzyl derivative, samples were analysed using combined capillary column gas chromatography negative ion chemical ionisation mass spectrometry [14].
• Methoxime analogs of selected beta-blockers were synthesized and their chemical stability established at different pH's. Subsequently, their in vivo sequential enzymatic conversion was confirmed using HPLC [15].

References