Disease relevance of Vinylite VGHH

• Foreign-body reactions were induced in coisogenic CBA/H and CBA/H-T6 mice by sc implantation of 15 times 22 times 0.2-mm unplasticized vinyl chloride vinyl acetate copolymer films [1].
• Foreign-body tumorigenesis by vinyl chloride vinyl acetate copolymer: no evidence for chemical cocarcinogenesis [2].
• Sarcomas were induced in 107 male and female isogeneic CBA/H or CBA/H-T6 mice by subcutaneous implantation of double films of unplasticized vinylchloride-acetate copolymer, 15 x 22 x 0.2 mm in size [3].
• A leukocidin was isolated and purified from autolysates of Pseudomonas aeruginosa strain 158 by a combination of procedures such as column chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-100, and zone electrophoresis on pevikon [4].

High impact information on Vinylite VGHH

• Guinea pig lymphotoxin, which was produced by stimulating immune lymph node cells with the specific antigen, ovalbumin, was partially purified by the sequential use of Pevikon block electrophoresis, ion exchange chromatography on DEAE-cellulose, and polyacrylamide disc gel electrophoresis [5].
• The liver cytosol containing 131I-T4 or T3, together with or without added stable hormones, was fractionated by Pevikon thin-layer electrophoresis at pH 8.6, 8.0, and7 [6].
• Plasma cholesterol levels were determined periodically and lipoproteins were separated terminally by density gradient ultracentrifugation, Pevikon block electrophoresis and immunoelectrophoresis [7].
• Using a correction assay a factor essential for normal deposition of C3 fragments on zymosan was identified in fractions of serum obtained by Sephacryl S-300 gel filtration (eluting between IgG and albumin), preparative Pevikon block electrophoresis (eluting in the beta-region) and DEAE-ion-exchange chromatography (eluting immediately post IgG) [8].
• An extract of Dermatophagoides pteronyssinus culture has been fractionated by chromatography on Sephadex G-100 and Pevikon block electrophoresis to obtain a partially purified allergen (F4P1) [9].

Anatomical context of Vinylite VGHH

• Several antigenic fractions could be purified from the mixture using gel filtration on Sephadex G-200 and block electrophoresis on Pevikon C870; two fractions were found to raise antibodies in a primary reaction and these antibodies effectively sensitized erythrocytes to lysis by complement [10].

Associations of Vinylite VGHH with other chemical compounds

• It behaves as a heat-stable pseudoglobulin, soluble in 20 per cent Na2SO4, and having alpha1 mobility on Pevikon block electrophoresis [11].

Gene context of Vinylite VGHH

• From the conditioned medium, D-factor was partially purified by preparative electrophoresis on a Pevikon block and the specific activity was increased 50 approximately 60-fold [12].
• Epidermal transglutaminase was purified by chromatography upon DEAE-Sephadex A-50, zone electrophoresis in Pevikon, and Sephadex G-200 gel permeation chromatography [13].

Analytical, diagnostic and therapeutic context of Vinylite VGHH

• Purification of melanoma-associated antigens was achieved by this step, followed by ion-exchange chromatography and preparative isoelectric focusing on Pevikon C870 [14].
• Plasma samples also were studied by zonal ultracentrifugation, gel permeation column chromatography, and Pevikon electrophoresis [15].
• The substrate was purified by DEAE-Sepharose CL-6B ion exchange chromatography, Pevikon block electrophoresis, and ACA-54 gel filtration chromatography [16].
• ZGM purification included preparative Pevikon electrophoresis, Sepharose 4B molecular exclusion chromatography and Con A-Sepharose affinity chromatography [17].

References