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Chemical Compound Review:

CHEMBL177629 [[[5-(4-amino-2-oxo- pyrimidin-1-yl)-3...

Synonyms: AR-1G0499, AC1L19NG, AC1Q6S3R, A24806

Roberts, D. et al., Taub, J.W. et al., Kawata, M. et al., Peng, L. et al., Szekeres, T. et al., et al.

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Disease relevance of deoxycytidine-triphosphate

DNA damage is measured by following the Escherichia coli DNA polymerase I-catalyzed incorporation of radiolabeled deoxycytidine triphosphate (dCTP) into the DNA of lysolecithin-permeabilized cells [1].

High impact information on deoxycytidine-triphosphate

Further study of the mechanisms (ie, alterations in dCTP pools and DNA methylation) involved may lead to improvements in the treatment of all AML patients [2].
Elevated ara-CTP levels in the DS cells were accompanied by slightly lower levels of endogenous deoxycytidine triphosphate (dCTP) pools, slightly greater extent of ara-C incorporation into DNA, and increased relative numbers of double strand DNA strand breaks [2].
Incubation of HL-60 cells with 50 mumol/L trimidox for 24 hours decreased deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) pools to 24% and 39% of control values, respectively [3].
When ara-C and MTX were administered together, levels of dTTP, dATP, and dGTP did not change significantly, and the increase in dCTP was only 25% of the increase after treatment with ara-C alone [4].
We suggest that MTX, by attenuating the ara-C-induced increase in dCTP, caused a change in the allosteric regulation of either deoxycytidine kinase or deoxycytidylate deaminase (or both), thereby potentiating the activity of ara-C [4].

Biological context of deoxycytidine-triphosphate

The addition of dTTP, dGTP, and dCTP reversed the inhibition of DNA repair by 2'-deoxycoformycin plus deoxyadenosine, although the level of dATP was not affected [5].

Anatomical context of deoxycytidine-triphosphate

The probe was 3'-end labeled with [3H] deoxycytidine triphosphate (dCTP) by using terminal deoxynucleotidyl transferase, and hybridization of the labeled probe to the mRNA in the rat hypothalamus was visualized autoradiographically [6].
Chinese hamster ovary (CHO) cells were subjected to electroporation in the presence of 5-methyl deoxycytidine-triphosphate [7].

Gene context of deoxycytidine-triphosphate

The method uses the enzyme terminal deoxynucleotidyl transferase (TDT) to incorporate labeled deoxycytidine triphosphate (dCTP) in the presence of dideoxy-CTP (ddCTP) which is added to ensure that the reaction goes to completion [8].

Analytical, diagnostic and therapeutic context of deoxycytidine-triphosphate

The quantitative determination of 2'-deoxycytidine-5'-triphosphate in cell extracts by radioimmunoassay [9].

References

Nick translation--a new assay for monitoring DNA damage and repair in cultured human fibroblasts. Snyder, R.D., Matheson, D.W. Environmental mutagenesis. (1985) [Pubmed]
Enhanced metabolism of 1-beta-D-arabinofuranosylcytosine in Down syndrome cells: a contributing factor to the superior event free survival of Down syndrome children with acute myeloid leukemia. Taub, J.W., Matherly, L.H., Stout, M.L., Buck, S.A., Gurney, J.G., Ravindranath, Y. Blood (1996) [Pubmed]
Synergistic growth inhibitory and differentiating effects of trimidox and tiazofurin in human promyelocytic leukemia HL-60 cells. Szekeres, T., Fritzer, M., Strobl, H., Gharehbaghi, K., Findenig, G., Elford, H.L., Lhotka, C., Schoen, H.J., Jayaram, H.N. Blood (1994) [Pubmed]
Effect of methotrexate and 1-beta-D-arabinofuranosylcytosine on pools of deoxyribonucleoside triphosphates in L1210 ascites cells. Roberts, D., Peck, C. Cancer Res. (1981) [Pubmed]
Factors influencing the inhibition of repair of irradiation-induced DNA damage by 2'-deoxycoformycin and deoxyadenosine. Begleiter, A., Verburg, L., Israels, L.G., Johnston, J.B. Cancer Chemother. Pharmacol. (1992) [Pubmed]
In situ hybridization histochemistry with oxytocin synthetic oligonucleotide: strategy for making the probe and its application. Kawata, M., McCabe, J.T., Pfaff, D.W. Brain Res. Bull. (1988) [Pubmed]
Gene silencing in mammalian cells by uptake of 5-methyl deoxycytidine-5'-triphosphate. Holliday, R., Ho, T. Somat. Cell Mol. Genet. (1991) [Pubmed]
A method for assessing strand breaks in DNA. Peng, L., Brisco, M.J., Morley, A.A. Anal. Biochem. (1998) [Pubmed]
The quantitative determination of 2'-deoxycytidine-5'-triphosphate in cell extracts by radioimmunoassay. Piall, E.M., Aherne, G.W., Marks, V. Anal. Biochem. (1986) [Pubmed]

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