Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

SBB006516 (3E)-3-[(3-carboxy-4-hydroxy- 5-methyl...

Synonyms:

This record was replaced with 3001009.

Pelludat, C. et al., Allard, K.A. et al., Laine, M.H. et al., Heesemann, J. et al., Feng, P. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of NSC623549

By immunoblotting using rabbit antibodies against Irp65 and chrome azurol S-agar, we were able to demonstrate that all tested mouse-lethal Y. enterocolitica and Yersinia pseudotuberculosis strains of different serotypes express siderophores and Irp65 [1].
Under iron stress, Legionella pneumophila secretes legiobactin, a nonclassical siderophore that is reactive in the chrome azurol S (CAS) assay [2].
Despite equal siderophore activities with various Pseudomonas spp. as measured by the chrome azurol S assay, the study shows how siderophore activity does not correlate with the antibacterial activity against food pathogens or with the antimould activity against pathogenic moulds [3].

High impact information on NSC623549

Thus, the core genes of Yen-HPI are sufficient to confer a positive CAS phenotype and mouse virulence to Y. enterocolitica MRS40, BG 2, but are insufficient to confer this phenotype to Y. enterocolitica NF-O, BG 1A [4].
Transfer of the resulting plasmid pCP1 into the siderophore-deficient strain Y. enterocolitica NF-O (BG 1A) led to no halo on siderophore indicator chrome azurol S (CAS) agar [4].
Complementation of the lbt mutants restored both siderophore expression, as measured by the CAS assay and bioassays, and bacterial growth in deferrated, citrate-containing media [2].
Relative to their wild-type parents, phcA mutants overproduced iron-scavenging activity detected with chrome azurol S siderophore detection medium [5].
The contents of HSA in blood serum samples were determined with the recovery of 97.1-102.3% and RSD of 0.6-2.9%, which are identical to those obtained according to the spectrofluorimetric method using chrome azurol S [6].

Biological context of NSC623549

To overcome the problem of instability, pCP1 was integrated into the chromosome of MRS40, leading to the formation of a CAS halo comparable to that seen with WA-C and correspondingly to increased mouse virulence [4].
Amonabactin biosynthetic mutants (generated by chemical mutagenesis) that either produced no amonabactin or overproduced the siderophore were isolated and identified on chrome azurol S siderophore detection agar [7].

Gene context of NSC623549

However, fyuA-dependent control of Ybt biosynthesis could be bypassed in a fyuA mutant by ingredients of chrome azurol S (CAS) siderophore indicator agar [8].
All strains of root nodule bacteria tested gave a positive CAS (chrome azurol S) assay for siderophore production [9].

Analytical, diagnostic and therapeutic context of NSC623549

Detection and differentiation of microbial siderophores by isoelectric focusing and chrome azurol S overlay [10].

References

Virulence of Yersinia enterocolitica is closely associated with siderophore production, expression of an iron-repressible outer membrane polypeptide of 65,000 Da and pesticin sensitivity. Heesemann, J., Hantke, K., Vocke, T., Saken, E., Rakin, A., Stojiljkovic, I., Berner, R. Mol. Microbiol. (1993) [Pubmed]
lbtA and lbtB are required for production of the Legionella pneumophila siderophore legiobactin. Allard, K.A., Viswanathan, V.K., Cianciotto, N.P. J. Bacteriol. (2006) [Pubmed]
Antimicrobial activity of Pseudomonas spp. against food poisoning bacteria and moulds. Laine, M.H., Karwoski, M.T., Raaska, L.B., Mattila-Sandholm, T.M. Lett. Appl. Microbiol. (1996) [Pubmed]
Transfer of the core region genes of the Yersinia enterocolitica WA-C serotype O:8 high-pathogenicity island to Y. enterocolitica MRS40, a strain with low levels of pathogenicity, confers a yersiniabactin biosynthesis phenotype and enhanced mouse virulence. Pelludat, C., Hogardt, M., Heesemann, J. Infect. Immun. (2002) [Pubmed]
Ralstonia solanacearum iron scavenging by the siderophore staphyloferrin B is controlled by PhcA, the global virulence regulator. Bhatt, G., Denny, T.P. J. Bacteriol. (2004) [Pubmed]
Direct quantification of human serum albumin in human blood serum without separation of gamma-globulin by the total internal reflected resonance light scattering of thorium-sodium dodecylbenzene sulfonate at water/tetrachloromethane interface. Feng, P., Huang, C.Z., Li, Y.F. Anal. Biochem. (2002) [Pubmed]
Physiological control of amonabactin biosynthesis in Aeromonas hydrophila. Barghouthi, S., Young, R., Arceneaux, J.E., Byers, B.R. Biology of metals. (1989) [Pubmed]
Functional analysis of yersiniabactin transport genes of Yersinia enterocolitica. Brem, D., Pelludat, C., Rakin, A., Jacobi, C.A., Heesemann, J. Microbiology (Reading, Engl.) (2001) [Pubmed]
Siderophore and organic acid production in root nodule bacteria. Carson, K.C., Holliday, S., Glenn, A.R., Dilworth, M.J. Arch. Microbiol. (1992) [Pubmed]
Detection and differentiation of microbial siderophores by isoelectric focusing and chrome azurol S overlay. Koedam, N., Wittouck, E., Gaballa, A., Gillis, A., Höfte, M., Cornelis, P. Biometals (1994) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.