Disease relevance of Dehydroergosterol

• We studied the transport of the fluorescent cholesterol analog dehydroergosterol (DHE) in polarized HepG2 human hepatoma cells [1].

High impact information on Dehydroergosterol

• Human NPC2 binds the cholesterol analog dehydroergosterol with submicromolar affinity at both acidic and neutral pH [2].
• By fluorescence imaging of the natural sterol dehydroergosterol (DHE), the lateral sterol distribution has been visualized in living cells [3]. The DHE transbilayer distribution in the plasma membrane and in endosomal recycling compartments (ERC) has been measured in living CHO cells by fluorescence imaging [4].
• However, addition of sterol carrier protein-2 in vitro significantly reduced the proportion of crystalline dehydroergosterol in the isolated lysosomes [5].
• The major proteins were identified as vitellogenins. rme-2 mutants, which lack the vitellogenin receptor, fail to accumulate dehydroergosterol in oocytes and embryos and instead accumulate dehydroergosterol in the body cavity along with vitellogenin [6].
• Dehydroergosterol accumulates primarily in the pharynx, nerve ring, excretory gland cell, and gut of L1-L3 larvae [6].

Biological context of Dehydroergosterol

• This signifies a reduction in energy transfer from dehydroergosterol to dansylated lecithin and indicates an increased separation between the two fluorophores [7].
• These kinetics were confirmed both by dehydroergosterol exchange measured with fluorescence intensity and by [3H]cholesterol exchange [8].
• The results show that cryptogein has one binding site with strong affinity for dehydroergosterol [9].

Anatomical context of Dehydroergosterol

• Fluorescent sterols, dehydroergosterol and NBD-cholesterol, were used to examine high density lipoprotein-mediated cholesterol uptake and intracellular targeting in L-cell fibroblasts [10].
• Later, the bulk of dehydroergosterol accumulates in oocytes and spermatozoa [6].
• A nonspecific phospholipid exchange protein (PLEP) preparation was used to transfer dansyl [3H]phosphatidylethanolamine (DNS-PE), dansyl[3H]phosphatidylserine (DNS-PS), and dehydroergosterol (DHE) from sonicated lipid vesicles to electroplax plasma membrane fragments enriched in Na+,K+-ATPase with retention of 80-90% of Na+,K+-ATPase activity [11].
• Incorporation of danyslated phospholipids and dehydroergosterol into membranes using a phospholipid exchange protein [11].
• Erythrocyte membrane lateral sterol domains: a dehydroergosterol fluorescence polarization study [12].

Associations of Dehydroergosterol with other chemical compounds

• Also, the fluorescent sterol dehydroergosterol binds to Bet v 1 as demonstrated by direct titrations [13].
• Dehydroergosterol binding produced sensitized emission of bound dehydroergosterol with longer lifetime.5) L-FABP bound two cis-parinaric acid molecules/molecule of protein [14].
• The transfer efficiency was 36%, and R, the apparent distance between the tyrosine energy donor and the dehydroergosterol energy acceptor, was 19 A. The significance of these data obtained in vitro for dehydroergosterol interaction with SCP was also tested in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)[15]
• Exposure to phospholipase C causes a temporary decrease in the dehydroergosterol to dansyl fluorescence ratio followed by an increase to and above the initial value [7].
• Third, the number of dehydroergosterol fluorescence lifetime components as well as the distribution in the cosonicated SUV was similar to that of dehydroergosterol in SUV composed of DMPC [16].

Gene context of Dehydroergosterol

• Dehydroergosterol bound to L-FABP with Kd = 0.37 microM and a stoichiometry of 0.83 mol/mol [14].
• The binding of dehydroergosterol to SCP-2 was evidenced by light scatter and by fluorescence polarization, lifetime, limiting anisotropy, and rotational relaxation time of dehydroergosterol [17].
• Binding analysis indicated SCP-2 bound [3H]cholesterol and dehydroergosterol with a Kd of 0.3 and 1.7 microM, respectively, and suggested the presence of a single binding site [18].
• The close molecular interaction of dehydroergosterol with SCP was also demonstrated by energy-transfer experiments [15].
• In contrast to component C1, the center of lifetime distribution, fractional intensity, and peak width of dehydroergosterol lifetime component C2 was dependent on the polarity of the medium and vesicle curvature [8].

Analytical, diagnostic and therapeutic context of Dehydroergosterol

• Human plasma LDL was labeled with dehydroergosterol and dansylated lecithin by incubation with donor multilamellar liposomes and isolated by centrifugation [19].
• The fluorescent sterol analogue delta 5,7,9(11),22-ergostatetraen-3 beta-ol (dehydroergosterol) was synthesized and purified by reverse-phase high-performance liquid chromatography [15].
• Thermal and dynamic properties of dehydroergosterol (DHE) in 1-stearoyl-2-capryl-sn-glycero-3-phosphocholine [C(18):C(10)PC] have been studied by differential scanning calorimetry (DSC) and multifrequency phase-modulation fluorometry [20].

References