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Chemical Compound Review:

AC1O5OJ6 (1S,5Z)-5-[(2E)-2- [(1R,3aR,7aR)-1-[(E,2S...

Synonyms: 1,25-(OH)2D2, 55248-15-2, 1,25-Dihydroxyvitamin D2, 1,25-Dihydroxyergocalciferol

Reinhardt, T.A. et al., Sato, K. et al., Braun, J. et al., Roux, S. et al., Sato, K. et al., et al.

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Disease relevance of 1,25-Dihydroxyergocalciferol

Chemical features of vitamin D deficiency were corrected in the presence of high circulating concentrations of 1,25-dihydroxyvitamin D2, produced endogenously, or of 1,25-dihydroxyvitamin D3, administered by mouth [1].
Scatchard analyses of 1,25-dihydroxyvitamin D receptors (VDR) from chick and rat intestine, bovine thymus, pig kidney cells (LLC-PK1), and human breast cancer cells (T-47D) demonstrated that 1,25-dihydroxyvitamin D3 (1,25-D3) and 1,25-dihydroxyvitamin D2 (1,25-D2) had equal affinities for VDR [2].
Progressively increased serum 1,25-dihydroxyvitamin D2 concentration in a hypoparathyroid patient with protracted hypercalcemia due to vitamin D2 intoxication [3].
Possible factors responsible for a protracted increase in serum 1,25-(OH)2D2 are body weight loss, hypoproteinemia, and phosphate depletion [3].

High impact information on 1,25-Dihydroxyergocalciferol

These data suggest that C-24 hydroxylation of 1,25-dihydroxyergocalciferol represents a significant in vivo deactivation step, whereas equivalent deactivation of 1,25-dihydroxycholecalciferol seems to involve metabolic steps subsequent to C-24 hydroxylation (C-24 ketonization) [4].
1,24-Dihydroxyvitamin D2 was also approximately 2-fold less competitive than either 1,25-dihydroxyvitamin D2 or 1,25-dihydroxyvitamin D3 for binding sites on the bovine thymus 1,25-dihydroxyvitamin D receptor [5].
The stability of the IgG fraction, the relatively short incubation interval, and the ability to measure 1,25-dihydroxycholecalciferol without interference from 1,25-dihydroxyergocalciferol are unique aspects of this radioimmunoassay [6].
This technique was successfully applied to evaluate differences in glucocorticoid receptor expression in U937 cells before and after the addition of potent differentiation inducers: 12-O-tetradecanoylphorbol 13-acetate (TPA) and a combination of all-trans retinoic acid (RA) and 1,25-dihydroxyvitamin D2 (VD) [7].
Comparison of receptor binding, biological activity, and in vivo tracer kinetics for 1,25-dihydroxyvitamin D3, 1,25-dihydroxyvitamin D2, and its 24 epimer [2].

Biological context of 1,25-Dihydroxyergocalciferol

It is concluded that senescence leads to reduced production of 1,25-dihydroxyvitamin D2 [1,25(OH)2D3] in untreated birds of both sexes and under both dietary conditions [8].

Anatomical context of 1,25-Dihydroxyergocalciferol

This paper reports that monocyte differentiation of HL-60 cells induced by 1,25 dihydroxyvitamin D2 (vitamin D2) results in a marked increase in expression of u-PA and u-PA-R [9].

Associations of 1,25-Dihydroxyergocalciferol with other chemical compounds

A monoclonal antibody has been used in a sensitive radioimmunoassay that measures 1,25-dihydroxyvitamin D2 (ercalcitriol) and 1,25-dihydroxyvitamin D3 (calcitriol) with equal potency [10].
AM were incubated with different concentrations of 1,25-(OH)2D2 (10(-11) to 10(-6) M) with and without priming with interferon-gamma (IFN-gamma) and with and without preincubation with 10(-8) M dexamethasone [11].

Gene context of 1,25-Dihydroxyergocalciferol

1,25-(OH)2D2 reduced TNF-alpha secretion; however, this difference was not significant [11].
Whether 1,25-(OH)2D2 alters the ICAM-1 expression of AM in humans has not been studied [11].
In addition, some bisphosphonates would certainly promote PTH-independent production of 1,25-(OH)2D2 [3].

Analytical, diagnostic and therapeutic context of 1,25-Dihydroxyergocalciferol

The concentration of dilute solutions of 1,25-dihydroxyvitamin D2 was determined by high-pressure liquid chromatography and deflection of the 254-nm column monitor [12].
When normocalcemia had been attained, intravenous hyperalimentation was started, in which 1,000 U vitamin D2 was inadvertently supplemented on days 5-18, On days 15-18, hypercalcemia rapidly recurred, accompanied by markedly increased serum levels of 25-OHD2 (9.1 ng/dl) and 1,25-(OH)2D2 (161 pg/ml) [13].

References

A familial syndrome of decrease in sensitivity to 1,25-dihydroxyvitamin D. Marx, S.J., Spiegel, A.M., Brown, E.M., Gardner, D.G., Downs, R.W., Attie, M., Hamstra, A.J., DeLuca, H.F. J. Clin. Endocrinol. Metab. (1978) [Pubmed]
Comparison of receptor binding, biological activity, and in vivo tracer kinetics for 1,25-dihydroxyvitamin D3, 1,25-dihydroxyvitamin D2, and its 24 epimer. Reinhardt, T.A., Ramberg, C.F., Horst, R.L. Arch. Biochem. Biophys. (1989) [Pubmed]
Progressively increased serum 1,25-dihydroxyvitamin D2 concentration in a hypoparathyroid patient with protracted hypercalcemia due to vitamin D2 intoxication. Sato, K., Emoto, N., Toraya, S., Tsushima, T., Demura, H., Tsuji, N., Inaba, S., Takeuchi, A., Kobayashi, T. Endocr. J. (1994) [Pubmed]
24-Hydroxylation of 1,25-dihydroxyergocalciferol. An unambiguous deactivation process. Horst, R.L., Reinhardt, T.A., Ramberg, C.F., Koszewski, N.J., Napoli, J.L. J. Biol. Chem. (1986) [Pubmed]
1 alpha-hydroxylation of 24-hydroxyvitamin D2 represents a minor physiological pathway for the activation of vitamin D2 in mammals. Horst, R.L., Koszewski, N.J., Reinhardt, T.A. Biochemistry (1990) [Pubmed]
A modified radioimmunoassay for 1,25-dihydroxycholecalciferol. Gray, T.K., McAdoo, T., Pool, D., Lester, G.E., Williams, M.E., Jones, G. Clin. Chem. (1981) [Pubmed]
Nonradioactive quantification of glucocorticoid receptor expression during differentiation of human monocytic cells (U937). Roux, S., Térouanne, B., Defacque, H., Vachier, I., Loubatière, J., Nicolas, J.C. Anal. Biochem. (1995) [Pubmed]
Vitamin D metabolism in aged Japanese quail: dietary calcium and estrogen effects. Baksi, S.N., Kenny, A.D. Am. J. Physiol. (1981) [Pubmed]
Urokinase inhibits HL-60 cell proliferation in vitro. Howell, A.L., Hunt, J.A., James, T.W., Mazar, A., Henkin, J., Zacharski, L.R. Blood Coagul. Fibrinolysis (1994) [Pubmed]
A sensitive radioimmunoassay using a monoclonal antibody that is equipotent for ercalcitriol and calcitriol (1,25-dihydroxy vitamin D2 and D3). Mawer, E.B., Berry, J.L., Cundall, J.P., Still, P.E., White, A. Clin. Chim. Acta (1990) [Pubmed]
1,25-dihydroxycholecalciferol stimulates ICAM-1 expression of human alveolar macrophages in healthy controls and patients with sarcoidosis. Braun, J., Dinkelacker, C., Bohnet, S., Kreft, B., Dalhoff, K. Lung (1999) [Pubmed]
Biological activity of 1,25-dihydroxyvitamin D2 in the chick. Jones, G., Baxter, L.A., DeLuca, H.F., Schnoes, H.K. Biochemistry (1976) [Pubmed]
Increased 1,25-(OH)2D2 concentration in a patient with malignancy-associated hypercalcemia receiving intravenous hyperalimentation inadvertently supplemented with vitamin D2. Sato, K., Imaki, T., Toraya, S., Demura, H., Tanaka, M., Kasajima, T., Takeuchi, A., Kobayashi, T. Intern. Med. (1993) [Pubmed]

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