Disease relevance of Oxonol-V

• Muscarinic depolarization of SH-SY5Y human neuroblastoma cells as determined using oxonol V [1].
• In some cases, as the animals were recovering from anoxia induced by nitrogen inhalation, oscillations similar to those due to spreading depression were observed in both the oxonol V and pyridine nucleotide signals [2].
• The plasma membrane potential of lymphocytes prepared from ataxia telangiectasia (AT) patients and normal subjects was assessed using the optical indicator bis-(3-phenyl-5-oxoisoxazol-4-yl) pentamethineoxonol (oxonol-V) [3].
• Generation of membrane potential (inside-positive) and delta pH (inside-acidic) at two kinds of NADH:quinone oxidoreductase segments, the Na(+)-motive segment and another segment, of Vibrio alginolyticus was examined by monitoring the quenching of fluorescence of oxonol V and that of quinacrine, respectively, with inside-out membrane vesicles [4].

High impact information on Oxonol-V

• Stimulation by ATP is not caused by the generation of a membrane potential, based on responses of the indicator dye oxonol V [5].
• Experiments in which fluorescent oxonol V was used as an indicator of a vesicle-interior positive membrane potential provided evidence for the electrogenicity of K+/H+ antiport and suggested that more than one H+ is exchanged for one K+ during a reaction cycle [6].
• Measurements with acridine orange and oxonol V indicate that the reconstituted ATPase retains its transport activity, generating both delta pH and delta psi during the hydrolysis of MgATP [7].
• Absorbance changes in the anionic dye bis[3-phenyl-5-oxoisoxazol-4-yl]pentamethineoxonol (oxonol V) can be used to monitor the membrane potential of liposomes and cytochrome c containing cytochrome oxidase proteoliposomes (c-loaded COV) [8].
• Respiration on internal cytochrome c generated a membrane potential of 53 mV (positive inside) and a pH gradient of 0.2 (acid inside) as monitored by the optical probes oxonol V and pyranine, respectively [9].

Biological context of Oxonol-V

• Measurements at the oxonol V fluorescence excitation wavelength indicated that only small changes in the reflectance signal occurred during seizure activity suggesting minimal blood volume change contributions to the extrinsic probe signal [2].
• Energization resulted in an increase in the number of oxonol-V binding sites, the new binding sites having the same dissociation constant [10].

Anatomical context of Oxonol-V

• In isolated plasma membrane vesicles, greater than 85% of which are inverted, ATP hydrolysis is accompanied by the formation of an inside acid pH gradient (delta pH) which can be detected by acridine orange fluorescence quenching and an inside positive membrane potential (delta psi) which can be detected by oxonol V fluorescence quenching [7].
• Synthesis, structure determination, spectral properties, and energy-linked spectral responses of the extrinsic probe oxonol V in membranes [11].
• The drug-induced oxonol V signal alterations have been provisionally interpreted as due at least in part to the reduction of the mitochondrial membrane potential that accompanies a state 4 to state 3 transition [2].
• Using surface fluorescence and reflectance measurements from the exposed cerebral cortex, several potential-sensitive molecular probes, primarily oxonol V, have been evaluated as indicators of electrical activity changes developing during seizure activity in the mongolian gerbil [2].
• Quantitative analysis of oxonol V fluorescence in submitochondrial particles [12].

Associations of Oxonol-V with other chemical compounds

• ATP-dependent vesicle acidification and membrane potential (psi) were measured using the fluorescent dyes acridine orange and Oxonol V (bis(3-phenyl-5-oxoisoxasol-4-yl)pentamethine oxonol), respectively [13].
• Protein I altered the surface membrane potential (Oxonol V): protein I evoked a transient membrane hyperpolarization which was not inhibited by furosemide [14].
• Lead entry generated an internally positive transmembrane potential, which could be detected by oxonol V fluorescence quenching, and dissipated a transmembrane pH gradient by alkalinization of the intravesicular space, as measured with pyranine [15].
• When succinate was added to inside-out vesicles, a membrane potential (inside positive) was generated, as indicated by fluorescence quenching of the anionic lipophilic dye Oxonol V [16].
• Measurements of membrane potential changes were made using the lipophilic ions, 3,3'-dipentyloxacarbocyanine (DiOC5(3] and bis(3-phenyl-5-oxoisoxazol-4-yl)pentamethineoxonol (oxonol V) [17].

Gene context of Oxonol-V

• The membrane potential changes were monitored by fluorescence microscopy using oxonol V; fluorescent probes were also used for F-actin and cadherin [18].

References