The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Chemical Compound Review

sORHEjpDIIm[P@     guanidine; thiocyanic acid

Synonyms: BIMB1010, NSC-2119, G9277_SIGMA, NSC2119, G6639_SIAL, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of NSC 2119

  • For preparation of virus RNA, the acid guanidinium-thiocyanate method was used [1].
  • A commercially available viral RNA extraction kit was used to isolate nucleic acids from captive gorilla fecal samples seeded with rotavirus and stored in ethanol, formalin, a commercial RNA preservation solution, guanidine thiocyanate buffer (GT), and samples dried in tubes containing silica gel [2].
 

High impact information on NSC 2119

  • Using competitive beta-globin PCR assays, one of the GTC-based, assays, provisionally named High Pure PCR Template Preparation (HPPTP) assay, yielded by far the highest quantity of amplifiable DNA [3].
  • Two of the methods were based on proteinase K treatment and two based on treatment with guanidinium thiocyanate (GTC) [3].
  • Most chorion cystatin of oocytes and ovulated eggs is solubilized by GTC, while a large amount of cystatin remains in the fertilization envelope of cortical reacted eggs after extraction by GTC [4].
  • Total RNA in the axotomized and control facial nuclear groups, dissected from the brain stem, was isolated by guanidinium-thiocyanate extraction and analyzed by northern hybridization and scanning densitometry [5].
  • IL-2, -10 and IFN transcripts were amplified from RNA isolated with guanidinium-thiocyanate from leukocytes stimulated with concanavalin A [6].
 

Anatomical context of NSC 2119

  • These findings demonstrated that isolation of total RNA with guanidinium-thiocyanate followed by DNase-treatment gave reliable and consistent results for detection of cytokine transcripts by RT-PCR in a canine macrophage cell line and canine peripheral blood leukocytes [6].
 

Associations of NSC 2119 with other chemical compounds

 

Gene context of NSC 2119

  • Total cellular RNA was isolated from gerbil brains by guanidine-thiocyanate extraction and characterized by northern blot analysis for c-fos, c-jun, and ODC mRNA over reperfusion times. c-fos and c-jun expression rose rapidly with peak level reached at 60 min of reperfusion (70 x control, p less than or equal to 0.01) [8].
 

Analytical, diagnostic and therapeutic context of NSC 2119

  • The quality of the DNA as measured by PCR assays amplifying different sizes of the beta-globin gene appeared to be superior for the GTC-based assays [3].
  • We conclude that, of these four methods, the GTC/silica method is the most appropriate for the extraction of viral RNA from faecal samples prior to RT-PCR for detecting SRSVs [9].

References

  1. Newcastle disease outbreaks in western China were caused by the genotypes VIIa and VIII. Liang, R., Cao, D.J., Li, J.Q., Chen, J., Guo, X., Zhuang, F.F., Duan, M.X. Vet. Microbiol. (2002) [Pubmed]
  2. Comparison of storage methods for reverse-transcriptase PCR amplification of rotavirus RNA from gorilla (Gorilla g. gorilla) fecal samples. Whittier, C.A., Horne, W., Slenning, B., Loomis, M., Stoskopf, M.K. J. Virol. Methods (2004) [Pubmed]
  3. A simplified and reliable HPV testing of archival Papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears. Jacobs, M.V., Zielinski, D., Meijer, C.J., Pol, R.P., Voorhorst, F.J., de Schipper, F.A., Runsink, A.P., Snijders, P.J., Walboomers, J.M. Br. J. Cancer (2000) [Pubmed]
  4. Identification of cystatin as a component of carp chorion. Chang, Y.S., Weng, J.W., Li, C.C., Huang, F.L. Mol. Reprod. Dev. (1998) [Pubmed]
  5. Induction of heat shock protein 70 mRNA in adult hamster facial nuclear groups following axotomy of the facial nerve. New, G.A., Hendrickson, B.R., Jones, K.J. Metabolic brain disease. (1989) [Pubmed]
  6. RT-PCR amplification of various canine cytokines and so-called house-keeping genes in a species-specific macrophage cell line (DH82) and canine peripheral blood leukocytes. Gröne, A., Fonfara, S., Markus, S., Baumgärtner, W. Zentralblatt Veterinarmedizin Reihe B (1999) [Pubmed]
  7. An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR. Finke, J., Fritzen, R., Ternes, P., Lange, W., Dölken, G. BioTechniques (1993) [Pubmed]
  8. Inducible ornithine decarboxylase expression in brain subject to vasogenic oedema after transient ischaemia: relationship to C-fos gene expression. Dempsey, R.J., Carney, J.M., Kindy, M.S. Acta neurochirurgica. Supplementum. (1990) [Pubmed]
  9. Comparison of four RNA extraction methods for the detection of small round structured viruses in faecal specimens. Hale, A.D., Green, J., Brown, D.W. J. Virol. Methods (1996) [Pubmed]
 
WikiGenes - Universities