Disease relevance of C00916

• The gene encoding a cephalosporin-C deacetylase (CAH) from Bacillus subtilis SHS 0133 was cloned and sequenced [1].
• Bacillus subtilis SHS0133 cephalosporin-C deacetylase (CAH) overexpressed in Escherichia coli was immobilized on an anion-exchange resin, KA-890, using glutaraldehyde [2].

High impact information on C00916

• Eliminating cystathionine-gamma-lyase prevents the enhancing precursor effect of methionine on cephalosporin-C production, and cystathionine-gamma-lyase overproduction in moderate doses increases cephalosporin-C formation [3].
• Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis [1].
• New conditional cell cycle mutants were isolated by selecting cephalosporin-C-resistant mutants of the beta-lactamase strain SC1107 at 30 degrees C that are also defective in cell division at 37 degrees C. They fall into two classes, represented by strains PC8002 and PC8003 [4].
• Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus [5].
• A spectrophotometric procedure for the assay of cephalosporin-C amidohydrolase activity, based on the determination of the 7-aminocephalosporanic acid (7-ACA) produced in the hydrolysis of cephalosporin-C by the enzyme, is described [6].

Biological context of C00916

• The whole cell immobilization technique is a potentially important process for Cephalosporin-C biosynthesis, where increase cell densities were maintained and broth-handling problems were reduced [7].
• To maximize the expression of the cephalosporin-C deacetylase (CAH) gene isolated from Bacillus subtilis SHS 0133 in Escherichia coli, a series of expression plasmids was constructed with various spacings between the Shine-Dalgarno sequence and the ATG initiation codon [8].

Analytical, diagnostic and therapeutic context of C00916

• Continuous production of cephalosporin-C by immobilized microbial cells using symbiotic mode in a packed bed bioreactor [7].

References