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Chemical Compound Review

IAA-94     2-[[(2S)-6,7-dichloro-2- cyclopentyl-2...

Synonyms: AC1LCVGT, AG-F-87436, CHEMBL1406302, BSPBio_001355, KBioGR_000075, ...
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Disease relevance of IAA-94

  • We confirmed that IAA-94 blocked IPC-, APNEA- and PMA-induced protection against infarction in the isolated heart model [1].
  • RESULTS: Indanyloxyacetic acid 94, a selective Cl(-) channel inhibitor that produced substantial inhibition of the regulatory volume decrease (RVD) when given at 10 microM concentration in cultured cardiomyocytes, was administered before ischemia to block RVD through Cl(-) channel inhibition [1].
  • CCPA, APNEA and PMA significantly (P<0.01) reduced the % of dead cardiomyocytes (by trypan blue staining) after 45 min SI/60 min SR, as compared to controls, while IAA-94 abolished this protection but did not affect PKCepsilon translocation by IPC [1].

High impact information on IAA-94

  • K+ channel blockers, Ba2+, and quinine strongly suppressed both the response in cell membrane potentials (Vb) and in (Cl-)i to the bath K+ step, while Cl- channel blockers, A9C (1 mM) and IAA-94 (0.3 mM) inhibited only the latter response by 49 and 74%, respectively [2].
  • Previously, using the ligand indanyloxyacetic acid (IAA), we purified four major proteins from bovine kidney cortex membrane vesicles [3].
  • In addition, these same antibodies were able to deplete IAA-94 inhibitable chloride channel activity from solubilized kidney membranes [3].
  • Specifically bound protein was eluted with 100 microM IAA-94 and either analyzed by SDS-gel electrophoresis or reconstituted into phospholipid vesicles [4].
  • It was inhibited by nickel and zinc ions in the micromolar range, but was unaffected by cobalt and had a low sensitivity to inhibition by the chloride channel blockers niflumic acid, DIDS, and IAA-94 [5].

Biological context of IAA-94


Anatomical context of IAA-94


Associations of IAA-94 with other chemical compounds


Gene context of IAA-94

  • Neither 4,4'dinitrostilbene-2,2'-disulfonic acid (100 microM) nor indanyloxyacetic acid 94 (50 microM) caused any change in either of the guinea pig ventricular currents [18].
  • Baseline diameter of afferent arterioles was not altered by 30 mumol/L IAA-94; however, AngII responsiveness was markedly attenuated [14].
  • A Cl(-)-channel inhibitor, IAA-94, blunted the depolarization and sustained elevation of [Ca2+]i in response to ET-1 [15].
  • Lowering of the extracellular Na+ concentration, but not IAA-94, a Cl- channel inhibitor, reduced both the PAR-1-mediated contraction and relaxation by about 50% [19].

Analytical, diagnostic and therapeutic context of IAA-94


  1. Pharmacological preconditioning in rabbit myocardium is blocked by chloride channel inhibition. Batthish, M., Diaz, R.J., Zeng, H.P., Backx, P.H., Wilson, G.J. Cardiovasc. Res. (2002) [Pubmed]
  2. Evidence for conductive Cl- pathway in the basolateral membrane of rabbit renal proximal tubule S3 segment. Seki, G., Taniguchi, S., Uwatoko, S., Suzuki, K., Kurokawa, K. J. Clin. Invest. (1993) [Pubmed]
  3. A ubiquitous 64-kDa protein is a component of a chloride channel of plasma and intracellular membranes. Redhead, C.R., Edelman, A.E., Brown, D., Landry, D.W., al-Awqati, Q. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  4. Indanyloxyacetic acid-sensitive chloride channels from outer membranes of skeletal muscle. Weber-Schürholz, S., Wischmeyer, E., Laurien, M., Jockusch, H., Schürholz, T., Landry, D.W., al-Awqati, Q. J. Biol. Chem. (1993) [Pubmed]
  5. A cyclic GMP-dependent calcium-activated chloride current in smooth-muscle cells from rat mesenteric resistance arteries. Matchkov, V.V., Aalkjaer, C., Nilsson, H. J. Gen. Physiol. (2004) [Pubmed]
  6. Epithelial chloride channel. Development of inhibitory ligands. Landry, D.W., Reitman, M., Cragoe, E.J., Al-Awqati, Q. J. Gen. Physiol. (1987) [Pubmed]
  7. Chloride channel blockers inhibit myogenic tone in rat cerebral arteries. Nelson, M.T., Conway, M.A., Knot, H.J., Brayden, J.E. J. Physiol. (Lond.) (1997) [Pubmed]
  8. Pharmacologic properties of the swelling-induced chloride current of dog atrial myocytes. Sorota, S. J. Cardiovasc. Electrophysiol. (1994) [Pubmed]
  9. Chloride channel blockade attenuates the effect of angiotensin II on tubuloglomerular feedback in WKY but not spontaneously hypertensive rats. Hashimoto, S., Kawata, T., Schnermann, J., Koike, T. Kidney Blood Press. Res. (2004) [Pubmed]
  10. Involvement of the intracellular ion channel CLIC1 in microglia-mediated beta-amyloid-induced neurotoxicity. Novarino, G., Fabrizi, C., Tonini, R., Denti, M.A., Malchiodi-Albedi, F., Lauro, G.M., Sacchetti, B., Paradisi, S., Ferroni, A., Curmi, P.M., Breit, S.N., Mazzanti, M. J. Neurosci. (2004) [Pubmed]
  11. Chloride-channel block inhibits T lymphocyte activation and signalling. Phipps, D.J., Branch, D.R., Schlichter, L.C. Cell. Signal. (1996) [Pubmed]
  12. Effect of frusemide, ethacrynic acid and indanyloxyacetic acid on spontaneous Ca-activated currents in rabbit portal vein smooth muscle cells. Greenwood, I.A., Hogg, R.C., Large, W.A. Br. J. Pharmacol. (1995) [Pubmed]
  13. Properties of K+ and Cl- channels and their involvement in proliferation of rat microglial cells. Schlichter, L.C., Sakellaropoulos, G., Ballyk, B., Pennefather, P.S., Phipps, D.J. Glia (1996) [Pubmed]
  14. Segment-specific effect of chloride channel blockade on rat renal arteriolar contractile responses to angiotensin II. Carmines, P.K. Am. J. Hypertens. (1995) [Pubmed]
  15. Intracellular signaling pathway of endothelin-1. Iijima, K., Lin, L., Nasjletti, A., Goligorsky, M.S. J. Cardiovasc. Pharmacol. (1991) [Pubmed]
  16. ATP-regulated chloride conductance in endoplasmic reticulum (ER)-enriched pig pancreas microsomes. Bégault, B., Anagnostopoulos, T., Edelman, A. Biochim. Biophys. Acta (1993) [Pubmed]
  17. Membrane currents elicited by the organic calcium channel blocker verapamil in native and rat brain RNA-injected oocytes of Xenopus laevis. Hübschle, T., Madeja, M., Musshoff, U., Speckmann, E.J. Arzneimittel-Forschung. (1997) [Pubmed]
  18. Effect of chloride channel blockers on the cardiac CFTR chloride and L-type calcium currents. Walsh, K.B., Wang, C. Cardiovasc. Res. (1996) [Pubmed]
  19. Characterization of the protease-activated receptor-1-mediated contraction and relaxation in the rat duodenal smooth muscle. Kawabata, A., Kuroda, R., Kuroki, N., Nishikawa, H., Kawai, K., Araki, H. Life Sci. (2000) [Pubmed]
  20. Identification and modulation of a voltage-dependent anion channel in the plasma membrane of guard cells by high-affinity ligands. Marten, I., Zeilinger, C., Redhead, C., Landry, D.W., al-Awqati, Q., Hedrich, R. EMBO J. (1992) [Pubmed]
  21. Clearance and micropuncture studies of the effects of a new indanyloxyacetic acid diuretic on segmental nephron function in rats. Kauker, M.L. J. Pharmacol. Exp. Ther. (1977) [Pubmed]
  22. Whole-cell chloride conductances in cultured brushed human nasal epithelial cells. Grygorczyk, R., Bridges, M.A. Can. J. Physiol. Pharmacol. (1992) [Pubmed]
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