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Chemical Compound Review

sodium ethanolate     sodium ethanolate

Synonyms:
 
 
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High impact information on ethanol

  • Biochemical experiments in which myelin or MAG was treated with sodium ethoxide and hydrogen peroxide, under conditions similar to those used for etching Epon sections prior to immune staining, showed that the immune reactivity of MAG was stable to these treatments [1].
  • Consistent specific staining for GFA protein in ultrathin sections mounted on nickel grids required partial removal of the plastic with 1% sodium ethoxide and further treatment with 2% sodium dodecyl sulfate (SDS) [2].
  • For thyroglobulin, the intensity was approximately 30% less in tissues that were exposed to sodium ethoxide [3].
  • The procedure using a Microprobe system in Eponembedded bone tissue with a sodium ethoxide concentration of 50% for two intervals of 20 min provided the best staining quality and tissue preservation [4].
  • Epon-araldite embedded tissues, the site of storage of somatostatin was localized with the peroxidaseantiperoxidase complexes technique, after removal of the resin by means of sodium ethoxide [5].
 

Anatomical context of ethanol

 

Associations of ethanol with other chemical compounds

 

Analytical, diagnostic and therapeutic context of ethanol

  • Antigen unmasking for immunoelectron microscopy: labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium [9].
  • Thin sections were immunogold-labeled for IgG after treatment with sodium ethoxide or sodium metaperiodate [9].
  • The use of sodium-ethoxide to remove plastic from tissue sections prior to immunohistochemistry resulted in the production of sufficient ssDNA to make a separate DNA denaturation step unnecessary, thus sparing sections from potentially destructive treatment and shortening the immunohistochemical procedure [10].

References

  1. Immunocytochemical localization of the myelin-associated glycoprotein. Fact or artifact? Trapp, B.D., Quarles, R.H. J. Neuroimmunol. (1984) [Pubmed]
  2. Post-embedding immunoperoxidase staining of glial fibrillary acidic protein for light and electron microscopy. DeArmond, S.J., Siegel, M.W., Dixon, R.G., Eng, L.F. J. Neuroimmunol. (1981) [Pubmed]
  3. How to examine the antigen-damaging effect of sodium ethoxide on deplasticized epoxy sections. Brorson, S.H. J. Histochem. Cytochem. (1997) [Pubmed]
  4. Pre-osteoblastic proliferation assessed with BrdU in undecalcified, Epon-embedded adult rat trabecular bone. Barou, O., Laroche, N., Palle, S., Alexandre, C., Lafage-Proust, M.H. J. Histochem. Cytochem. (1997) [Pubmed]
  5. Immuno-electron-cytochemical localization of the somatostatin cells in the human antral mucosa. Canese, M.G., Bussolati, G. J. Histochem. Cytochem. (1977) [Pubmed]
  6. On-grid immunogold labeling of glial intermediate filaments in epoxy-embedded tissue. Johnson, A.B., Bettica, A. Am. J. Anat. (1989) [Pubmed]
  7. Post-surface conditioning improves interfacial adhesion in post/core restorations. Monticelli, F., Toledano, M., Tay, F.R., Cury, A.H., Goracci, C., Ferrari, M. Dental materials : official publication of the Academy of Dental Materials. (2006) [Pubmed]
  8. Immunocytochemical localization of delta 3, delta 2-enoyl-CoA isomerase and NADPH-dependent-2,4-dienoyl-CoA reductase in rat kidney. Yokota, S., Tomioka, Y., Suzuki, H., Mizugaki, M. Histochemistry (1993) [Pubmed]
  9. Antigen unmasking for immunoelectron microscopy: labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium. Stirling, J.W., Graff, P.S. J. Histochem. Cytochem. (1995) [Pubmed]
  10. Bromodeoxyuridine (BrdUrd) immunohistochemistry in undecalcified plastic-embedded tissue. Elimination of the DNA denaturation step. Apte, S.S., Puddle, B. Histochemistry (1990) [Pubmed]
 
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