Disease relevance of GUANOSINE DIPHOSPHATE

• Three experiments have examined the interaction of adrenalectomy and fenfluramine on food intake, body weight and the binding of guanosine-5'-diphosphate (GDP) to interscapular brown adipose tissue (IBAT) [1].
• 5'-GDP and 5'-GMP were both equally effective in potentiating the antitumor activity of FUra without increasing toxicity [2].

High impact information on GUANOSINE DIPHOSPHATE

• Regulation of DNA synthesis by guanosine-5'-diphosphate, cyclic guanosine-3',5'-monophosphate, and cyclic adenosine-3', 5'-monophosphate in mouse lymphoid cells [3].
• Fucosyltransferases (FucTs), present in both eukaryotic and prokaryotic organisms, are the enzymes responsible for the catalysis of fucose transfer from donor guanosine-diphosphate fucose to various acceptor molecules including oligosaccharides, glycoproteins, and glycolipids [4].
• Our data showed that label added as [3H]-GTP, [3H]-GDP, or [3H]-GMP was readily taken up and concentrated in the matrix of isolated mitochondria [5].
• The method involves adding [3H]-GTP to microtubules at steady state, and analysing for non-exchangeable [3H]-GDP in the presence of a GTP regenerating system [6].
• To evaluate these possibilities, the oxygen consumption of female Sprague-Dawley rats aged 5 and 26 months was recorded at rest and during 6 h of exposure to 6 degrees C. The thermogenic capacity of brown fat was estimated from the binding of guanosine-5'-diphosphate (GDP) to isolated brown fat mitochondria [7].

Biological context of GUANOSINE DIPHOSPHATE

• To clarify the effect of caffeine on brown adipose tissue (BAT) thermogenesis, we measured guanosine-5'-diphosphate (GDP) binding, a thermogenic indicator of BAT, and oxygen consumption in BAT mitochondria as well as BAT temperature and resting metabolic rate (RMR) in mice [8].

Anatomical context of GUANOSINE DIPHOSPHATE

• Sympathetic nervous system activity was assessed by measuring the binding of the purine nucleotide guanosine-5'-diphosphate (GDP) to mitochondria isolated from IBAT [9].
• It is concluded that for agonists which act on receptors which function through G-proteins these pharmacological parameters may depend on the concentration of the relevant G-protein in the cell membranes and on the intracellular concentrations of guanosine-5'-triphosphate and guanosine-5'-diphosphate [10].
• BRL 26830A administration significantly increased guanosine-5'-diphosphate (GDP)-binding in brown adipose tissue (BAT) and the resting metabolic rate (RMR), and significantly reduced retroperitoneal white adipose tissue (WAT) pads in both groups [11].
• After density gradient separation of erythrocyte populations into cell age-dependent fractions, the concentrations of adenosine-5'-triphosphate, guanosine-5'-triphosphate and guanosine-5'-diphosphate were be found to be elevated by 25-100% in all cell fractions from haemodialysis patients, compared with a healthy control group [12].

Associations of GUANOSINE DIPHOSPHATE with other chemical compounds

• When benidipine hydrochloride was incorporated in the diet (1.0 mg per gram of food) for 4 weeks, binding of guanosine-5'-diphosphate in brown adipose tissue mitochondria was significantly increased [13].
• BAT function was assessed by measuring sympathetic nervous system (SNS) activity in BAT (estimated by the rate of norepinephrine (NE) turnover), BAT mitochondrial content (estimated by cytochrome c oxidase activity), and BAT mitochondrial proton conductance (estimated by guanosine-5'-diphosphate (GDP) binding to isolated BAT mitochondria) [14].

Gene context of GUANOSINE DIPHOSPHATE

• The hydrodynamics of the bacterial elongation factor EF-Tu have been studied in the presence of its ligand guanosine-5'-diphosphate (GDP) by sedimentation in the ultracentrifuge and quasielastic light scattering [15].
• All of the agents indicated above induced changes in the expression of moesin, rho guanosine-5'-diphosphate-dissociation inhibitor, and actin, confirmed by immunoblotting and confocal laser microscopy [16].

Analytical, diagnostic and therapeutic context of GUANOSINE DIPHOSPHATE

• Surgical denervation of the five sympathetic nerves supplying one lobe of the interscapular brown fat of control rats caused small reductions in mass, protein content and the activity of the thermogenic mitochondrial proton conductance pathway (assessed from guanosine-5'-diphosphate (GDP) binding) when compared to the intact lobe [17].

References