Disease relevance of Desoxycholic acid

• Because the composition of faeces modulates colorectal carcinogenesis, promotional effects of the secondary bile salt sodium deoxycholate (SDC) were compared with those of dilute homogenised faeces (12.5% w/v) or saline alone in rat colon isolated from the faecal stream as a Thiry-Vella fistula (TVF) [1].
• Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis [2].
• The extraction of Treponema pallidum antigen with sodium desoxycholate, based on a previously described procedure (J. Portnoy and H.J. Magnuson, J. Immuno. 75:348-355, 1955), was used in an enzyme-linked immunosorbent assay (ELISA) test for syphilis [2].
• A series of experiments was designed to test the ability of sodium deoxycholate (SDC) to promote intestinal hyperplasia and neoplasia in rats (n = 265) [3].
• Myoelectrical activity was recorded in rats after induction of severe acute pancreatitis (SAP) using sodium deoxycholate (SDOC) [4].

High impact information on Desoxycholic acid

• A method was developed based on the measurement of constitutive beta-glucosidase (esculinase) with the repression of this enzyme by bile equivalent (sodium desoxycholate) that required only 30 min. p-Nitrophenyl-beta-D-glucopyranoside was the esculinase substrate, and sodium desoxycholate was substituted for bile salts [5].
• METHODS AND RESULTS: This medium, designed to detect strains belonging to the clone of VTEC O157:H7/H-, contains carbohydrates and two chromogenic substrates to detect beta-d-galactosidase and beta-d-glucuronidase and sodium desoxycholate to increase selectivity for Gram-negative rods [6].
• While sodium desoxycholate was found to down-regulate C. jejuni flaA promoter activity, L-fucose was found to up-regulate its activity [7].
• CONCLUSIONS: Among the enhancers tested, 10 mM sodium desoxycholate and HP-beta-CD appear to be viable candidates for further development of an oral formulation of cosalane and its congeners [8].
• We found that phosphatidylethanolamine, rather than phosphatidylcholine, was the preferred substrate, the Ca2+ ion was essential for the expression of PLA2 activity, the enzyme was active over a broad pH range, with the optimum at pH 9.0, and sodium-deoxycholate inhibited the enzyme activity strongly in a concentration-dependent manner [9].

Chemical compound and disease context of Desoxycholic acid

• Metronidazole reduced this tumour promotion without affecting SDC-induced hyperplasia [3].

Anatomical context of Desoxycholic acid

• We conclude that the sodium-deoxycholate-induced intestinal secretion is partly caused by the activation of local nervous reflexes [10].
• The study evaluated the effect of 2% sodium desoxycholate combined with whole plasma from a single donor on gingival fibroblast attachment to diseased root surfaces [11].
• However, these compounds failed to permeate the small intestine unless sodium desoxycholate (5-20 mM) was used as an intestinal permeation enhancer [12].
• Rough Brucella strains B. abortus (45/20), B. ovis (1182), wild type B. canis (M+) and a less mucoid (M-) variant possessed cell wall antigens that were extracted by both sodium desoxycholate (SDC) and hot phosphate buffered saline (PBS) [13].
• Daily samples were lysed with sodium desoxycholate, and inoculated onto 7H11 agar plates for the subsequent colony-forming units (CFU)/ml count [14].

Associations of Desoxycholic acid with other chemical compounds

• 0. Restriction of colonies was most effective with 0.7% sorbose, 0.1% sodium-desoxycholate, and 0.006% griseofulvin in the medium adjusted to pH 6 [15].
• The contractility of jejunum segments was evaluated in the presence of SDOC, lipopolysaccharide (LPS) and trypsin.QYT accelerated the transit in MAP mice in a concentration dependent manner [4].
• The agent was found to be resistant to chloroform, ether, trypsin, sodium desoxycholate, oxytetracycline and a pH range of three to nine [16].
• The dissolution media tested were 0.02% polysorbate 80 in water (pH = 6--7), sodium desoxycholate (10 mmol/1) in water (pH = 7.2) and 0.02% polysorbate 80 in HCl (0.1 mol/l, pH = 2) [17].

Gene context of Desoxycholic acid

• The sodium deoxycholate (SDO) solubilized proteinic material, fractionated on Concanavalin A [Con A] and injected to CBA mice simultaneously with A/J spleen cells caused a suppression of the hemagglutinating titer and C-mediated cytotoxicity (IgG2) [18].
• The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b. braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease [19].
• For the bile salt sodium desoxycholate in ammonia: sodium bicarbonate, pH 8.2, the Qm with cholestyramine was 4641 +/- 2669 and with Super Char was 2814 +/- 667 (p = 0.11) [20].

Analytical, diagnostic and therapeutic context of Desoxycholic acid

• The MEKC method involved the use of 70 mM sodium deoxycholate (SDC) in 40 mM borate (pH 9.2) buffer and UV detection at 300 nm [21].
• A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented [22].

References