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Gene Review

DNAJA2  -  DnaJ (Hsp40) homolog, subfamily A, member 2

Homo sapiens

Synonyms: CPR3, Cell cycle progression restoration gene 3 protein, DJ3, DJA2, DNAJ, ...
 
 
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Disease relevance of DNAJA2

  • We have identified two estrogen regulated gene products in the E(2) growth inhibited human breast cancer xenograft, T61; one showing 100% homology to the human BAC clone RP11-112E16, the other 100% homology to the human CPR3/DNJ3 gene [1].
 

High impact information on DNAJA2

  • Addition of the radical scavenger DMSO increased levels of CYP2D6 holoenzyme in DUKX/2D6/CPR-3 cells but not in DUKX/2D6 cells [2].
  • Subsequently, DUKX/2D6 cells were transfected with human CPR cDNA to generate the cell line DUKX/2D6/CPR-3 [2].
  • In agreement with this, the activity of haem oxygenase, a rate-limiting enzyme in haem metabolism, was compared with that in DUKX/DHFR control cells (expressing dihydrofolate reductase), and was 3-fold higher in DUKX/2D6/CPR-3 but similar in DUKX/2D6 cells [2].
  • SV40 17KT antigen complements dnaj mutations in large T antigen to restore transformation of primary human fibroblasts [3].
  • OBJECTIVE: Methylation of a CpG island within the Methylation controlled DNAJ (MCJ) gene results in loss of expression in normal and neoplastic cells [4].
 

Biological context of DNAJA2

 

Anatomical context of DNAJA2

  • Heat shock enhances the amount of prenylated Dnaj protein at membranes of glyoxysomes [6].
  • Cell fractionation of etiolated cotyledons showed that Dnaj proteins were detectable mainly in the postnuclear cell fraction after sedimentation at 10,000 x g, and in the microsomes [6].
 

Associations of DNAJA2 with chemical compounds

  • Administration of radiolabelled mevalonic acid to cotyledons and isolation of glyoxysomes yielded labelled Dnaj protein which remained membrane bound during the purification of glyoxysomal membranes by floatation in a density gradient [6].
  • Treatment of T61 tumors with tamoxifen, leading to static tumor growth, also increased the expression of the BAC clone RP11-112E16 and the CPR3/DNJ3 mRNAs [1].
 

Analytical, diagnostic and therapeutic context of DNAJA2

  • Plant dnaj homologue: molecular cloning, bacterial expression, and expression analysis in tissues of cucumber seedlings [7].
  • When glyoxysomes were treated with protease and subsequently isolated by gradient centrifugation, virtually all immunologically detectable Dnaj protein was removed [6].

References

  1. Identification of two estrogen regulated genes associated with growth regulation of human breast cancer. Ohlsson, H., Brünner, N., Engelholm, L.H., Lundholt, B.K., Weidle, U., Briand, P., Lykkesfeldt, A.E. Mol. Cell. Endocrinol. (2001) [Pubmed]
  2. Human NADPH-P450 oxidoreductase modulates the level of cytochrome P450 CYP2D6 holoprotein via haem oxygenase-dependent and -independent pathways. Ding, S., Yao, D., Deeni, Y.Y., Burchell, B., Wolf, C.R., Friedberg, T. Biochem. J. (2001) [Pubmed]
  3. SV40 17KT antigen complements dnaj mutations in large T antigen to restore transformation of primary human fibroblasts. Boyapati, A., Wilson, M., Yu, J., Rundell, K. Virology (2003) [Pubmed]
  4. Demethylation of the MCJ gene in stage III/IV epithelial ovarian cancer and response to chemotherapy. Strathdee, G., Vass, J.K., Oien, K.A., Siddiqui, N., Curto-Garcia, J., Brown, R. Gynecol. Oncol. (2005) [Pubmed]
  5. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Ohtsuka, K., Hata, M. Cell Stress Chaperones (2000) [Pubmed]
  6. Heat shock enhances the amount of prenylated Dnaj protein at membranes of glyoxysomes. Preisig-Müller, R., Muster, G., Kindl, H. Eur. J. Biochem. (1994) [Pubmed]
  7. Plant dnaj homologue: molecular cloning, bacterial expression, and expression analysis in tissues of cucumber seedlings. Preisig-Müller, R., Kindl, H. Arch. Biochem. Biophys. (1993) [Pubmed]
 
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