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Gene Review

pheU  -  tRNA

Escherichia coli CFT073

 
 
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Disease relevance of pheU

 

High impact information on pheU

  • Using proteomic and microarray analysis, we identified for the first time that AggR activates the expression of chromosomal genes, including 25 contiguous genes (aaiA-Y), which are localized to a 117 kb pathogenicity island (PAI) inserted at pheU [3].
  • Three plasmids were shown to carry the structural gene for tRNAPhe which we call pheU [4].
  • Recombinant plasmids harboring a single direct repeat cloned either with or without the LEE O26 integrase gene were found to insert within the chromosomal pheU locus of E. coli K-12 strains with equal efficiency, suggesting that an endogenous P4-like integrase can substitute for this activity [5].
  • Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA [6].
  • PCR also showed that the LEE (locus of enterocyte effacement) was inserted in selC in four isolates, likely in pheU in seven isolates, and in undetermined sites in twelve isolates [7].
 

Biological context of pheU

  • The pheU gene for tRNA(Phe) under control of the native tRNA promoter was on a multicopy plasmid and gave up to 40-fold overproduction of tRNA(Phe)U39 [8].
  • Both the pheU gene and the pheST genes encoding the alpha and beta subunits of phenylalanyl-tRNA synthetase were located on the physical map of the T. thermophilus chromosome, where pheU was present on a hitherto unrecognized HpaI DNA fragment [2].
  • Our results indicate that pheU/pheV-located genomic islands played an important role in the evolution of several PAls in E. coli and related pathogens [9].
 

Anatomical context of pheU

  • Sixty five (72.2%) of the eae+ isolates had insertion of the locus of enterocyte effacement locus in the pheU locus, 11 (12.2%) in the selC and 14 (15.6%) did not insert in either of these loci [10].
 

Associations of pheU with chemical compounds

 

Analytical, diagnostic and therapeutic context of pheU

  • By restriction enzyme analysis, DNA blotting and DNA:tRNA hybridization, pheU was localised to a 280 bp fragment within a 5.6 kb PstI restriction fragment of E.coli DNA [4].

References

  1. Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. Schwartz, I., Klotsky, R.A., Elseviers, D., Gallagher, P.J., Krauskopf, M., Siddiqui, M.A., Wong, J.F., Roe, B.A. Nucleic Acids Res. (1983) [Pubmed]
  2. Cloning and sequencing of the pheU gene for tRNA(Phe) of Thermus thermophilus HB8, and genomic mapping of the pheU and pheST genes. Keller, B., Hennecke, H. FEMS Microbiol. Lett. (1994) [Pubmed]
  3. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Dudley, E.G., Thomson, N.R., Parkhill, J., Morin, N.P., Nataro, J.P. Mol. Microbiol. (2006) [Pubmed]
  4. Identification of clones carrying an E. coli tRNAPhe gene by suppression of phenylalanyl-tRNA synthetase thermosensitive mutants. Caillet, J., Plumbridge, J.A., Springer, M., Vacher, J., Delamarche, C., Buckingham, R.H., Grunberg-Manago, M. Nucleic Acids Res. (1983) [Pubmed]
  5. Active genetic elements present in the locus of enterocyte effacement in Escherichia coli O26 and their role in mobility. Muniesa, M., Schembri, M.A., Hauf, N., Chakraborty, T. Infect. Immun. (2006) [Pubmed]
  6. Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA. Gallagher, P.J., Schwartz, I., Elseviers, D. J. Bacteriol. (1984) [Pubmed]
  7. Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E. coli (EPEC). Nakazato, G., Gyles, C., Ziebell, K., Keller, R., Trabulsi, L.R., Gomes, T.A., Irino, K., Da Silveira, W.D., Pestana De Castro, A.F. Vet. Microbiol. (2004) [Pubmed]
  8. 1H-15N NMR studies of Escherichia coli tRNA(Phe) from hisT mutants: a structural role for pseudouridine. Davis, D.R., Poulter, C.D. Biochemistry (1991) [Pubmed]
  9. Dissemination of pheU- and pheV-located genomic islands among enteropathogenic (EPEC) and enterohemorrhagic (EHEC) E. coli and their possible role in the horizontal transfer of the locus of enterocyte effacement (LEE). Rumer, L., Jores, J., Kirsch, P., Cavignac, Y., Zehmke, K., Wieler, L.H. Int. J. Med. Microbiol. (2003) [Pubmed]
  10. Serobiotypes and virulence genes of Escherichia coli strains isolated from diarrheic and healthy rabbits in Brazil. Penteado, A.S., Ugrinovich, L.A., Blanco, J., Blanco, M., Blanco, J.E., Mora, A., Andrade, J.R., Corrêa, S.S., Pestana de Castro, A.F. Vet. Microbiol. (2002) [Pubmed]
 
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