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Gene Review:

rpoB - DNA-directed RNA polymerase, beta subunit

Bacillus anthracis str. Ames

Qi, Y. et al., Ellerbrok, H. et al., Wahab, T. et al., Zasada, A.A. et al.

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Disease relevance of rpoB

We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis [1].
Some Bacillus thuringiensis strains share rpoB nucleotide polymorphisms also present in Bacillus anthracis [2].

High impact information on rpoB

We have developed a real-time PCR-based assay for the specific detection of B. anthracis by taking advantage of the unique nucleotide sequence of the B. anthracis rpoB gene [3].
A TaqMan real-time PCR for identification of B. anthracis was developed, based on the two plasmids, pX01 and pX02, both of which are necessary for pathogenicity, as well as on the chromosomally encoded rpoB gene [4].

Analytical, diagnostic and therapeutic context of rpoB

Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis [3].

References

Pyrosequencing Bacillus anthracis. Wahab, T., Hjalmarsson, S., Wollin, R., Engstrand, L. Emerging Infect. Dis. (2005) [Pubmed]
Some Bacillus thuringiensis strains share rpoB nucleotide polymorphisms also present in Bacillus anthracis. Zasada, A.A., Gierczynski, R., Raddadi, N., Daffonchio, D., Jagielski, M. J. Clin. Microbiol. (2006) [Pubmed]
Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis. Qi, Y., Patra, G., Liang, X., Williams, L.E., Rose, S., Redkar, R.J., DelVecchio, V.G. Appl. Environ. Microbiol. (2001) [Pubmed]
Rapid and sensitive identification of pathogenic and apathogenic Bacillus anthracis by real-time PCR. Ellerbrok, H., Nattermann, H., Ozel, M., Beutin, L., Appel, B., Pauli, G. FEMS Microbiol. Lett. (2002) [Pubmed]

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