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Gene Review

SF3A3  -  splicing factor 3a, subunit 3, 60kDa

Homo sapiens

Synonyms: PRP9, PRPF9, SAP 61, SAP61, SF3a60, ...
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High impact information on SF3A3

  • Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 [1].
  • We report the isolation of a cDNA encoding SAP 61 and find that it is 30% identical in amino acid sequence to PRP9 [1].
  • We have analyzed the function of individual subunits of human SF3a (SF3a60, SF3a66, and SF3a120) by testing recombinant proteins, expressed in insect cells, in various in vitro assays [2].
  • By contrast, SF3a60 and SF3a66 lacking the zinc finger domains accumulate in Cajal bodies and are diffusely distributed in the cytoplasm, suggesting a function for Cajal bodies in the final maturation of the U2 snRNP [3].
  • SURP2 exhibits a fold of the alpha1-alpha2-3(10)-alpha3 topology, and the SF3a60 fragment forms an amphipathic alpha helix intimately contacting alpha1 of SURP2 [4].

Biological context of SF3A3

  • Regions in SF3a60 and SF3a66 that mediate the binding to SF3a120 are necessary for nuclear import of the proteins, suggesting that the SF3a heterotrimer forms in the cytoplasm [3].

Analytical, diagnostic and therapeutic context of SF3A3


  1. Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. Chiara, M.D., Champion-Arnaud, P., Buvoli, M., Nadal-Ginard, B., Reed, R. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  2. Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. Nesic, D., Krämer, A. Mol. Cell. Biol. (2001) [Pubmed]
  3. A role for Cajal bodies in the final steps of U2 snRNP biogenesis. Nesic, D., Tanackovic, G., Krämer, A. J. Cell. Sci. (2004) [Pubmed]
  4. Solution Structures of the SURP Domains and the Subunit-Assembly Mechanism within the Splicing Factor SF3a Complex in 17S U2 snRNP. Kuwasako, K., He, F., Inoue, M., Tanaka, A., Sugano, S., G??ntert, P., Muto, Y., Yokoyama, S. Structure (2006) [Pubmed]
  5. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. Wiest, D.K., O'Day, C.L., Abelson, J. J. Biol. Chem. (1996) [Pubmed]
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