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Gene Review:

dnaK - molecular chaperone DnaK

Streptomyces coelicolor A3(2)

Synonyms: SCH35.53, SCH44.11c

Bucca, G. et al., Bucca, G. et al., Duchêne, A.M. et al., Bucca, G. et al., Brans, A. et al.

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Disease relevance of dnaK

Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2) [1].

High impact information on dnaK

Here we report in vivo DnaK depletion experiments which demonstrate that DnaK is a negative regulator of the dnaK operon [2].
Significantly, analysis of the entire genome sequence revealed that the promoter regions of dnaK, clpB and lon are the only sequences that contain the HspR consensus binding sequence 5'-TTGAGY-N7-ACTCAA [2].
Previous in vitro studies demonstrated that DnaK forms a specific complex with HspR bound to its operator sequences in dnaKp, and a model was proposed in which DnaK functions as a corepressor of the dnaK operon (Bucca, G., Brassington, A., Schonfeld, H.J., and Smith, C.P. (2000) Mol Microbiol 38: 1093-1103) [2].
The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationally coupled [1].
The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively [1].

Biological context of dnaK

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70) [3].
The dnaK operon of Streptomyces coelicolor encodes the DnaK chaperone machine and HspR, the transcriptional repressor of the operon; HspR confers repression by binding to several inverted repeat sequences in the promoter region, dnaKp [4].
The dnaK operon of Streptomyces coelicolor encodes the DnaK chaperone machine and the negative autoregulator HspR, which confers repression of the operon by binding to several inverted repeat sequences in the promoter region, dnaKp [2].

Analytical, diagnostic and therapeutic context of dnaK

Instead, regulation of these genes from vegetative-type promoters may be effected by a DNA-binding protein observed in gel retardation assays, which recognizes a motif found in the groE and dnaK promoter regions of many prokaryotic genes [5].

References

Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2). Bucca, G., Smith, C.P., Alberti, M., Seidita, G., Passantino, R., Puglia, A.M. Gene (1993) [Pubmed]
Negative feedback regulation of dnaK, clpB and lon expression by the DnaK chaperone machine in Streptomyces coelicolor, identified by transcriptome and in vivo DnaK-depletion analysis. Bucca, G., Brassington, A.M., Hotchkiss, G., Mersinias, V., Smith, C.P. Mol. Microbiol. (2003) [Pubmed]
Cloning and sequencing of the dnaK locus in Streptomyces coelicolor A3(2). Brans, A., Loriaux, A., Joris, B., Dusart, J. DNA Seq. (1996) [Pubmed]
The HspR regulon of Streptomyces coelicolor: a role for the DnaK chaperone as a transcriptional co-repressordagger. Bucca, G., Brassington, A.M., Schönfeld, H.J., Smith, C.P. Mol. Microbiol. (2000) [Pubmed]
Transcriptional analysis of groEL genes in Streptomyces coelicolor A3(2). Duchêne, A.M., Thompson, C.J., Mazodier, P. Mol. Gen. Genet. (1994) [Pubmed]

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