Gene Review:
ndhK - NADH dehydrogenase subunit B
Nostoc sp. PCC 7120
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Disease relevance of ndhK
- This indicates that under the selection conditions used, ndhK is an essential gene in Anabaena PCC 7120 [1].
- This is markedly different from the case in Synechocystis PCC 6803 where a 71-bp non-coding, intergenic spacer region lies between ndhC and ndhK [1].
High impact information on ndhK
- The coding regions of ndhC and ndhK overlap by 7 bp, as found in the chloroplasts of liverwort, maize, and rice [1].
- The nucleotide sequence of ndhK encodes a protein of 245 amino acids with a predicted molecular mass of 27.5 kDa [1].
- Cloning, analysis and inactivation of the ndhK gene encoding a subunit of NADH quinone oxidoreductase from Anabaena PCC 7120 [1].
- The ndhK clone was interrupted by the insertion of a kanamycin-resistance gene and used to transform Anabaena PCC 7120.20 unsegregated transformants were produced, all of which died during attempts to segregate them [1].
References
- Cloning, analysis and inactivation of the ndhK gene encoding a subunit of NADH quinone oxidoreductase from Anabaena PCC 7120. Howitt, C.A., Whelan, J., Price, G.D., Day, D.A. Eur. J. Biochem. (1996) [Pubmed]
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