Disease relevance of dnaJ

• Cloning, nucleotide sequence, and regulatory analysis of the Lactococcus lactis dnaJ gene [1].
• Cloning, nucleotide sequence and structural analysis of the Clostridium acetobutylicum dnaJ gene [2].

High impact information on dnaJ

• The transcription initiation site of the dnaJ gene was determined and appeared to be preceded by a typical gram-positive vegetative promoter sequence (TTGCCA-17 bp-TAAAAT) [1].
• The dnaJ gene of Lactococcus lactis was isolated from a genomic library of L. lactis NIZO R5 and cloned into pUC19 [1].
• Deletion mutagenesis showed that the inverted repeat is involved in heat shock regulation of the dnaJ gene [1].
• A transcriptional fusion between the dnaJ expression signals and a usp45-amyS secretion cassette caused a significant increase in alpha-amylase activity after heat shock induction [1].
• Nucleotide sequencing of a positively reacting 2.2-kb HincII fragment, contained in the recombinant plasmid pKG4, revealed that the reading frame of the dnaJ gene of C. acetobutylicum consists of 1125 bp, encoding a protein of 374 amino acids with a calculated M(r) of 40376 and an isoelectric point of 9.54 [2].

Biological context of dnaJ

• The complete dnaJ gene of Clostridium acetobutylicum was isolated by chromosome walking using the previously cloned 5' end of the gene as a probe [2].

References