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Gene Review:

Atu0696 - phosphatase

Agrobacterium fabrum str. C58

Kalogeraki, V.S. et al., Brencic, A. et al., Tiburtius, A. et al., Melchers, L.S. et al., Thorstenson, Y.R. et al., et al.

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High impact information on Atu0696

First, we show that VirA has a transmembrane topology by analysis of the alkaline phosphatase activities, determined by several virA-phoA gene fusions [1].
DIG3 encodes an enzymatically active type 2C serine/threonine protein phosphatase [2].
The fact that wild-type VirA can actively block vir gene expression in the absence of phenolic signals suggests that it might have a phospho-VirG phosphatase activity [3].
In addition to facilitating measurements of gene expression, some plasmids create translational fusions to beta-galactosidase or alkaline phosphatase and are therefore useful in studying the membrane topology of a target protein [4].
The observation that a virB8::TnphoA fusion restores export of alkaline phosphatase to the periplasm suggests that VirB8 spans the inner membrane [5].

Regulatory relationships of Atu0696

TnphoA insertions into exoY which expressed alkaline phosphatase activity were isolated and mapped, confirming the membrane location of the exoY gene product [6].

References

Membrane topology and functional analysis of the sensory protein VirA of Agrobacterium tumefaciens. Melchers, L.S., Regensburg-Tuïnk, T.J., Bourret, R.B., Sedee, N.J., Schilperoort, R.A., Hooykaas, P.J. EMBO J. (1989) [Pubmed]
Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2. Tao, Y., Rao, P.K., Bhattacharjee, S., Gelvin, S.B. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
VirA of Agrobacterium tumefaciens is an intradimer transphosphorylase and can actively block vir gene expression in the absence of phenolic signals. Brencic, A., Xia, Q., Winans, S.C. Mol. Microbiol. (2004) [Pubmed]
Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria. Kalogeraki, V.S., Winans, S.C. Gene (1997) [Pubmed]
The essential virulence protein VirB8 localizes to the inner membrane of Agrobacterium tumefaciens. Thorstenson, Y.R., Zambryski, P.C. J. Bacteriol. (1994) [Pubmed]
Expression of the exoY gene, required for exopolysaccharide synthesis in Agrobacterium, is activated by the regulatory ros gene. Tiburtius, A., de Luca, N.G., Hussain, H., Johnston, A.W. Microbiology (Reading, Engl.) (1996) [Pubmed]

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