Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

Atu5042 - recombinase

Agrobacterium fabrum str. C58

Gleave, A.P. et al., Vergunst, A.C. et al., Dale, E.C. et al., Yamamoto, A. et al., Vergunst, A.C. et al., et al.

Vergunst, van Lier, den Dulk-Ras, Hooykaas, Vergunst, Jansen, Hooykaas,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Atu5042

Flanked by recombination sites from the bacteriophage P1 Cre/lox recombination system, the hpt gene was subsequently excised from the plant genome by the Cre recombinase [1].
Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase [2].

High impact information on Atu5042

Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells [3].
We employed the earlier developed CRAFT (Cre recombinase Reporter Assay For Translocation) assay to detect transfer of Cre::Vir fusion proteins from A. tumefaciens into plants, monitored by stable reconstitution of a kanamycin resistance marker, and into yeast, screened by loss of the URA3 gene [4].
Retransformation of these plants with pCre1 (containing 35S transcribed cre recombinase and hygromycin (hpt) resistance genes) resulted in excision of the loxP-flanked genes from the genome [5].
Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene [5].
We propose that product(s) of this gene have recombinase or endonuclease activity which specifically recognizes the 25 bp terminal repeats of T-DNA [6].

References

Gene transfer with subsequent removal of the selection gene from the host genome. Dale, E.C., Ow, D.W. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase. Vergunst, A.C., Jansen, L.E., Hooykaas, P.J. Nucleic Acids Res. (1998) [Pubmed]
VirB/D4-dependent protein translocation from Agrobacterium into plant cells. Vergunst, A.C., Schrammeijer, B., den Dulk-Ras, A., de Vlaam, C.M., Regensburg-Tuïnk, T.J., Hooykaas, P.J. Science (2000) [Pubmed]
Recognition of the Agrobacterium tumefaciens VirE2 translocation signal by the VirB/D4 transport system does not require VirE1. Vergunst, A.C., van Lier, M.C., den Dulk-Ras, A., Hooykaas, P.J. Plant Physiol. (2003) [Pubmed]
Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Gleave, A.P., Mitra, D.S., Mudge, S.R., Morris, B.A. Plant Mol. Biol. (1999) [Pubmed]
The promoter proximal region in the virD locus of Agrobacterium tumefaciens is necessary for the plant-inducible circularization of T-DNA. Yamamoto, A., Iwahashi, M., Yanofsky, M.F., Nester, E.W., Takebe, I., Machida, Y. Mol. Gen. Genet. (1987) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.