Disease relevance of Tpt1

• Glutathione ester prevents buthionine sulfoximine-induced cataracts and lens epithelial cell damage [1].

High impact information on Tpt1

• When cultured at low density, however, they died by apoptosis, suggesting that they depend on other lens epithelial cells for their survival [2].
• Conditioned medium from high density cultures promoted the survival of cells cultured at low density, suggesting that lens epithelial cells support one another's survival by secreting survival factors [2].
• MIP was not detectable in the basal or lateral plasma membrane of the lens epithelial cell, including the interepithelial cell gap junctions; nor was MIP detectable in the plasma membrane or gap junctions of the hepatocyte [3].
• AR was localized in the following structures in the eye: lens epithelial lining and cortical lenticular fibers; corneal endothelium; the inner, nonpigmented layer of ciliary body epithelium and its extension as the posterior surface of the iris; and neuroglial (Müller) cells in the retina [4].
• The demethylation of the promoter region was shown to occur during the differentiation from the lens epithelial to the lens fiber cell [5].

Chemical compound and disease context of Tpt1

• Evaluation of lens epithelial cell differentiation by quantitation of MP26 mRNA relative to gamma-crystallin mRNA in initiation of galactose cataracts in the rat [6].

Biological context of Tpt1

• Identification of the calcium binding sites in translationally controlled tumor protein [7].
• We conclude that the strong expression of TCTP in spermatogonia makes it highly likely that the protein plays a significant role in spermatogenesis [8].
• Western and Northern blot analyses confirmed the presence of TCTP at all ages studied, with higher levels of RNA expression at 9 and 20 d postpartum, when spermatogonia and primary spermatocytes represent the highest proportion of germ cells: it was also confirmed that TCTP is present in all populations of isolated testicular cells [8].
• METHODS: bFGF-induced cell proliferation was monitored in rat lens epithelial explants by bromodeoxyuridine (BrdU) incorporation [9].
• This suggested that up-regulation of Egr-1 mRNA may be involved in lens epithelial cell death [10].

Anatomical context of Tpt1

• Cellular distribution of translationally controlled tumor protein in rat and human testes [8].
• In addition, different levels of TCTP expression were also observed in the testicular somatic cells, with strong expression in Leydig cells and peritubular cells, and weak expression in Sertoli cells [8].
• The evidence presented here suggests that the composition of the fiber cell junction and plasma membrane is also immunocytochemically distinct from that of its progenitor, the lens epithelial cell [3].
• METHODS: The effects of lovastatin on lens transparency and on lens epithelial cell proliferation and structure have been investigated using organ-cultured rat lenses and cultured epithelial cells from human and rabbit lenses, respectively [11].
• The molecular form of uptake of GSH in lens epithelial mRNA-injected oocytes was examined by high-performance liquid chromatography [12].

Associations of Tpt1 with chemical compounds

• Translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor, is a growth-related tumor protein [7].
• Here, we reported that the Ca2+ binding region of TCTP, which was mapped by using a combination of deletion constructs of rat TCTP and 45Ca2+ -overlay assay, was confined to amino acid residues 81-112 [7].
• GTP-binding proteins accumulated in the soluble fractions of lovastatin-treated lens epithelial cells [11].
• Supplementation of the medium with DL-mevalonic acid (a precursor of isoprenoids whose synthesis is inhibited by lovastatin) prevented the lovastatin-induced changes in whole lenses or in lens epithelial cell cultures, whereas supplementation with cholesterol had no such effect [11].
• Rat lens epithelial explants were cultured with sodium selenite [10].

Regulatory relationships of Tpt1

• Structural analysis of lens epithelial explants induced to differentiate into fibres by fibroblast growth factor (FGF) [13].

Other interactions of Tpt1

• Using an immunohistochemistry technique, an intense TCTP signal was detected in gonocytes (the prespermatogonia) in the fetal rat testis and in spermatogonia within adult human and neonatal and adult rat testes [8].
• Age-related changes in fibre differentiation of rat lens epithelial explants exposed to fibroblast growth factor [14].
• The diminished ability of lens epithelial explants from older rats to undergo fibre specific changes in response to bFGF is also demonstrated in the reduced degree of morphological changes characteristic of fibre differentiation [14].
• METHODS: PDGF-AA, EGF, NGF, M-CSF, BMP-2, BMP-4, dexamethasone, and estrogen were tested for their ability to alter the spectrum of crystallins in explanted newborn rat lens epithelial cells or in vitro differentiating newborn rat lens fiber cells [15].
• Therefore, the pathogenesis of MNU-induced cataract was associated with DNA adduct formation in the lens epithelial cell nuclei leading to apoptosis by upregulation of Bax protein, downmodulation of Bcl-2 protein, and activation of caspase-3 [16].

Analytical, diagnostic and therapeutic context of Tpt1

• In organ culture of rat eye anterior segments, anti-PDGF-D strongly inhibits lens epithelial cell proliferation [17].
• Light and electron microscopy were used to assess rat lens epithelial explants cultured with bovine aqueous or vitreous [18].
• In the peripheral in vitro GPI bioassay, TCTP, TCTOP, and TCTAP were highly effective antagonists of the potent mu opioid receptor agonist PL017, with pA2 = 8.69 for TCTAP, 8.10 for TCTP, and 7.38 for TCTOP [19].
• METHODS: An mRNA differential display method was used to identify genes with altered expression during differentiation of lens epithelial cells [20].
• Cultures with or without DEX (100 nM), and appropriate controls, were maintained for up to 30 days and assessed by light and scanning electron microscopy or immunolocalization of PCO markers (alpha-smooth muscle actin or fibronectin) or a marker for lens epithelial cell phenotype (Pax-6) [21].

References